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Measurement method for non-fat organic acids in tobacco and tobacco product

A method of determination and technology of tobacco products, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low detection sensitivity, analysis and research that have not been reported in the literature, and the inability to separate organic acids, etc., to achieve the effect of high sensitivity

Active Publication Date: 2019-01-15
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, the analysis of 3-hydroxypropionic acid, m-toluic acid, 4-oxononanoic acid, 2-hydroxybenzoic acid and cinnamic acid in tobacco and tobacco products has not been reported in the literature
At the same time, due to the limitation of the sensitivity of the instrument, some non-fatty organic acids with low content, such as cinnamic acid, were not detected by the existing GC-MS method.
Therefore, when using the prior art to analyze non-fatty organic acids in tobacco and tobacco products, it is usually impossible to effectively separate the organic acids whose content is disclosed, and the detection sensitivity is low

Method used

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  • Measurement method for non-fat organic acids in tobacco and tobacco product
  • Measurement method for non-fat organic acids in tobacco and tobacco product
  • Measurement method for non-fat organic acids in tobacco and tobacco product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041]The assay method of the non-fatty organic acid in tobacco and tobacco products of the present embodiment is simultaneously to lactic acid, levulinic acid, 2-furan formic acid, 3-hydroxy propionic acid, benzoic acid, phenylacetic acid, m-methylbenzene Formic acid, 4-oxononanoic acid, 2-hydroxybenzoic acid, cinnamic acid and other 10 kinds of non-fatty organic acids are determined, including the following steps:

[0042] 1) Grind the tobacco leaf sample into powder, pass through a 40-mesh sieve, and obtain the smoke powder under the sieve; weigh 0.5 g of the tobacco powder as the sample to be tested and place it in a sample bottle, then add 3 mL of dichloromethane extractant, and then add 50 μL of 20 μg / mL trans-2-hexenoic acid internal standard solution, ultrasonic extraction for 30min to obtain the extract; filter the extract through the organic phase membrane;

[0043] 2) Take 0.5 mL of the extract from the organic phase filter, then add 200 μL of bis(trimethylsilyl) t...

Embodiment 2

[0051] The assay method of non-fatty organic acids in the tobacco and tobacco products of the present embodiment comprises the following steps:

[0052] Select one C3F sample of flue-cured tobacco, burley tobacco, oriental tobacco, and sun-cured red tobacco in four different types of tobacco leaves, and use the determination method in Example 1 to measure lactic acid, levulinic acid, 2-furan formic acid, and 3-hydroxypropanoic acid in the samples to be tested. Determination of the content of 10 non-fatty organic acids such as acid, benzoic acid, phenylacetic acid, m-toluic acid, 4-oxononanoic acid, 2-hydroxybenzoic acid, and cinnamic acid.

[0053] Ten non-fatty organic acids (lactic acid, levulinic acid, 2-furancarboxylic acid, 3-hydroxypropionic acid, benzoic acid, phenylacetic acid, m-toluic acid, 4-oxononanoic acid, 2- Hydroxybenzoic acid, cinnamic acid) content results are shown in Table 2.

[0054] Table 2 Contents of 10 non-fatty organic acids in different types of tob...

Embodiment 3

[0057] The assay method of non-fatty organic acids in tobacco and tobacco products of the present embodiment is also simultaneously lactic acid, levulinic acid, 2-furan formic acid, 3-hydroxypropionic acid, benzoic acid, phenylacetic acid, m-toluene in tobacco leaf The content of 10 kinds of non-fat organic acids such as formic acid, 4-oxononanoic acid, 2-hydroxybenzoic acid, cinnamic acid is measured, and the specific measuring method is except that dichloromethane in step 1) is replaced by normal hexane, and In step 2), the heating program is replaced by the following heating program, and all the other are completely the same as in Example 1;

[0058] Heating program: keep at 40°C for 3 minutes, then rise to 160°C at a rate of 10°C / min, then rise to 190°C at a rate of 10°C / min, and finally rise to 280°C at a rate of 25°C / min, and hold for 25 minutes.

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Abstract

The invention relates to a measurement method for non-fat organic acids in tobacco and a tobacco product and belongs to the technical field of analysis of acidic components in the tobacco and the tobacco product. The measurement method provided by the invention comprises the steps of extracting a to-be-measured sample, and taking extract liquid for derivative reaction to obtain to-be-measured liquid; taking the to-be-liquid, and carrying out gas chromatography-tandem mass spectrometry, wherein a gas chromatography condition comprises the following heating program of keeping 40-60 degrees centigrade for 1-3 min, heating to 140-160 degrees according to 3-10 degrees centigrade / min, heating to 190-210 degrees centigrade according to 5-10 degrees centigrade / min, heating to 270-290 degrees centigrade according to 20-30 degrees centigrade / min, and keeping warm for 15-25 min. According to the measurement method provided by the invention, various non-fat organic acids can be effectively separated, sensitivity is high, and the measurement method can be used for measuring content of the organic acids such as 3-hydroxypropionic acid, m-toluic acid, 4-oxononanoic acid, 2-hydroxybenzoic acid andcinnamic acid.

Description

technical field [0001] The invention relates to a method for determining non-fatty organic acids in tobacco and tobacco products, and belongs to the technical field of analysis of acid components in tobacco and tobacco products. Background technique [0002] Organic acids widely exist in tobacco, with a content of 10% to 16%, and play an important role in the aroma and taste of cigarettes. The most studied organic acids in tobacco leaves are fatty acids, such as volatile fatty acids (formic acid, acetic acid, propionic acid, etc.) and higher fatty acids (undecanoic acid, oleic acid, linolenic acid, etc.). For non-fatty organic acids, such as lactic acid, levulinic acid, 2-furancarboxylic acid, 3-hydroxypropionic acid, benzoic acid, phenylacetic acid, m-toluic acid, 4-oxononanoic acid, 2-hydroxybenzoic acid, There are few studies on cinnamic acid and so on. However, these non-fatty organic acids have characteristic aroma and taste, which play a very important role in the fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/7206G01N2030/025G01N2030/062G01N2030/067
Inventor 余晶晶王冰薛聪张晓兵蔡君兰赵晓东刘克建秦亚琼刘绍锋刘惠民
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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