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Application of lead and cadmium mixing exposure detection in assistant diagnosis of azoospermia

A technology for azoospermia and auxiliary diagnosis, applied in the fields of analytical chemistry and clinical medicine, to achieve the effect of improving sensitivity and specificity, easy detection, delaying and preventing disease progression

Inactive Publication Date: 2019-01-15
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, especially in the field of auxiliary diagnosis of azoospermia, the technology of using ICP-MS to analyze the mixed exposure level of lead and cadmium in male whole blood has not been developed

Method used

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  • Application of lead and cadmium mixing exposure detection in assistant diagnosis of azoospermia
  • Application of lead and cadmium mixing exposure detection in assistant diagnosis of azoospermia
  • Application of lead and cadmium mixing exposure detection in assistant diagnosis of azoospermia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1 Research object selection and grouping basis

[0083] The inventor collected whole blood samples of qualified azoospermia cases and normal control men from Nanjing Maternity and Child Health Hospital affiliated to Nanjing Medical University. Semen examination and normal fertility were used as controls, and semen examination without sperm was used as cases. 120 cases were randomly included, and 120 control males were matched according to age, BMI, and abstinence time. A total of 240 people were used as experimental subjects for the assessment of mixed exposure to lead and cadmium in azoospermia. The specific sample classification standards are as follows:

[0084] The first stage of association exploration stage

[0085] A total of 120 men were included in the case, and 120 controls were matched according to age, BMI, and abstinence time, totaling 240.

[0086] Group A: healthy control group (120 people, normal semen examination and fertility):

[0087] 1. Between 22 ...

Embodiment 2I

[0100] Example 2 ICP-MS detection of lead and cadmium mixed exposure and analysis of azoospermia

[0101] 1. Sample preparation

[0102] Take 100μl of whole blood, add 4.9ml reagent A (that is, diluted 50 times), and mix well.

[0103] 2. Instrument testing

[0104] Analysis instrument: ICAP RQ ICP-MS (Thermo)

[0105] a. After the ICP-MS is ignited, it is stable for about half an hour; pump 1ppb tuning solution (provided by the instrument manufacturer, Ba, Bi, Ce, Co, Ln, Li, U, each concentration is 1μg / L, the matrix is ​​2.5 %HNO 3 , 0.5% HCl), according to the sensitivity of Li, In and U, oxide (CeO) and double charge (Ba 2+ ), tune the entire mass range (4~290amu) and achieve the best state; the tuning requirements meet the following standards: standard mode sensitivity 7Li≥4000cps / ppb, 115In≥15000cps / ppb; 238U≥20000cps / ppb; standard mode Double charge Ba 2+ / Ba <3.0%; standard mode oxide 156CeO / 140Ce <2.0%; The optimized operating parameters are shown in Table 1.1;

[0106] Table...

Embodiment 3

[0119] Example 3 Preparation of a kit for detecting mixed exposure of lead and cadmium in male whole blood to assist in the diagnosis of azoospermia

[0120] First, the ICP-MS method was used to determine the higher levels of lead and cadmium in the whole blood of the case group, and then the chi-square test found that the composition ratio of individuals with different exposure levels in the healthy control group and the case group was significantly different. There were more people with high exposure than the control group, and high exposure to lead and cadmium significantly increased the incidence of azoospermia. Detection of mixed exposure of lead and cadmium can be used for auxiliary diagnosis of azoospermia. This kit includes a batch of reagents and consumables for the detection of lead and cadmium in whole blood. The diluent is used for sample dilution, and it contains a stable and detectable internal standard for experimental quality control. The concentration gradient of...

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Abstract

The invention belongs to the field of analytical chemistry and clinical medicine, and discloses an application of lead and cadmium mixing exposure detection in an assistant diagnosis of azoospermia. An exposed biomarker associated with the azoospermia is the mixing exposure of the lead and the cadmium. The concentration of the lead and the cadmium in whole blood can be detected by ICP-MS (Inductively Coupled Plasma Mass Spectrometry), which can be used for the assistant diagnosis of the azoospermia. The application of the lead and the cadmium mixing exposure detection in the assistant diagnosis of the azoospermia has the advantages of less blood volume, simple operation, fast, accurate, and good clinical promotion value.

Description

Invention field [0001] The present invention belongs to the field of analytical chemistry and clinical medicine, and relates to an exposure biomarker related to azoospermia and a detection method and application thereof. The exposure biomarker is lead-cadmium mixed exposure, and its detection method is based on ICP-MS Detection method. Background technique [0002] Globally, the proportion of couples of childbearing age who suffer from infertility is about 8% to 15%. At present, the incidence of infertility in my country is as high as 15% to 20%, and male factors account for 30%. The research of the WHO infertility control panel shows that: reproductive endocrine dysfunction, especially poor semen quality, is an important cause of male infertility. Among male infertility patients, azoospermia caused by gene mutations accounts for more than 30%. Spermatogenesis is a complex physiological process characterized by the orderly proliferation and differentiation of cell types, that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N33/50
CPCG01N27/626G01N33/50
Inventor 夏彦恺张明智卢婷徐俏俏董天宇陈敏健王心如
Owner NANJING MEDICAL UNIV
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