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A liquid submerged fermentation process for high-yielding Armillaria armillaria

A technology of submerged liquid fermentation and armillaria, applied in the direction of fungi, methods based on microorganisms, methods using microorganisms, etc., to achieve the effect of industrialized production and optimization of fermentation processes

Active Publication Date: 2021-08-31
CHANGCHUN SHENGJINNUO BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This research comprehensively utilizes the above main technologies to study the screening of high-yield strains of Armillaria armillaria and large-scale fermentation production process, and this excellent strain has a variety of biological activities, which has not been reported at home and abroad.

Method used

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  • A liquid submerged fermentation process for high-yielding Armillaria armillaria
  • A liquid submerged fermentation process for high-yielding Armillaria armillaria
  • A liquid submerged fermentation process for high-yielding Armillaria armillaria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 High-yield Armillaria domestication selection and identification

[0061] 1.1 Preparation of medium

[0062] PDA medium: 200g of potatoes, 20g of glucose, 10.1g of vitamin b, 2% agar powder, add deionized water and boil for 30min, filter, and dilute to 1000ml. After aliquoting, sterilize at 115°C for 30 minutes.

[0063] Seed medium (unit: g / L): sucrose 20, glucose 10, yeast extract powder 10, peptone 10, potassium dihydrogen phosphate 1.5, magnesium sulfate 0.75, vitamin B10.01, dilute to 1000ml with deionized water, pH nature. After aliquoting, sterilize at 115°C for 30 minutes.

[0064] Basic fermentation medium (unit: g / L): Edible glucose 20, soybean meal powder 20, potassium dihydrogen phosphate 1, magnesium sulfate 1, sodium chloride 1, vitamin b 10.01, dilute to 1000ml with deionized water, pH nature. After aliquoting, sterilize at 115°C for 30 minutes.

[0065] 1.2 Culture method

[0066] Activation of strains: Fill 100mL shake flask with 40mL o...

Embodiment 2

[0071] Identification of embodiment 2 high-yield Armillaria mellea strains

[0072] The selected strains of Armillaria mellifera were entrusted to the Microbial Testing Center of China Food and Fermentation Industry Research Institute for identification, and the identification was carried out by observing the morphological characteristics, DNA sequence analysis of ITSrDNA and β-tubulin gene segments, and proved that the strains obtained through domestication and breeding were Armillaria genus Armillaria mellea.

Embodiment 3

[0073] The drawing of embodiment 3 high-yield armillaria growth curves

[0074] Shake flasks were used to cultivate the high-yield Armillaria mellifera strain, and 16 Erlenmeyer flasks with a specification of 500ml were taken, numbered 1, 2, 3, 4, 5, 6...16. Use a measuring cylinder to accurately measure 200ml of deionized water into a weighed conical flask, stir evenly, and after heating, sterilize in a high-pressure steam boiler at 115°C. The culture conditions are temperature of 26°C, inoculum size of 5%, rotation speed of 150 rpm, and fermentation time of 120 h. The initial exploration of the shake flask growth curve in this experiment started at 0h, and the mycelium dry weight, intracellular polysaccharide and exopolysaccharide content were measured every 24h. The growth curve medium formula is shown in Table 1.

[0075] Table 1 Preparation of Armillaria growth curve medium

[0076]

[0077] It can be seen from Table 2 that before 72 hours, with the increase of cultu...

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Abstract

The invention provides a liquid submerged fermentation process for high-yield Armillaria armillaria, comprising the following steps: step 1: domestication and breeding of high-yield Armillaria armillaria; step 2: identification of high-yield Armillaria armillaria; step 3: establishment of high-yield Armillaria armillaria seed fermentation process Step 4: The fermentation process of the high-yield Armillaria fermenter is established; Step 5: Based on the above steps one to four, the high-yield Armillaria 200L fermenter is scaled up and verified, and the liquid submerged fermentation process of a high-yield Armillaria can Realize the use of soybean meal as a nitrogen source to optimize the fermentation process of Armillaria armillaria, and realize the industrial production of Armillaria armillaria.

Description

technical field [0001] The invention mainly relates to a high-yield armillaria liquid submerged fermentation process. Background technique [0002] Armillaria armillaria is a kind of fungi that are both edible and medicinal. As a fungus, its fruiting body is delicious and rich in nutrients. It is a low-fat, high-protein, rich in cellulose, vitamins, inorganic salts and Polysaccharide health food. Sun Xiaowei et al found that there are 17 kinds of amino acids that can be detected in Armillaria cordula, among which leucine has the highest content, and other essential amino acids account for about 35% of the total amino acids. Among non-essential amino acids, glutamic acid and Aspartic acid has the largest share, and these two amino acids are beneficial to the brain. In addition, Armillaria armillae cords contain K, Ca, P, Mg and other constant elements needed by the human body, and other elements such as Cu, Zn, Fe, Mn, and Ge also have relatively high contents. [0003] Ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/36C12R1/645
CPCC12N1/14C12N1/36
Inventor 张军徐婷杜研李少华楚志洋刘加岐关羽虹张婷王聪
Owner CHANGCHUN SHENGJINNUO BIOLOGICAL PHARMA
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