Method for hybrid breeding of white-petal rapeseed through brassica napus recessive GMS line and radish

Abstract

The invention belongs to the technical field of rapeseed breeding and discloses a method for hybrid breeding of white-petal rapeseed through brassica napus recessive GMS line and radish. The method comprises the following steps: selection of an original parent: selecting stable recessive GMS line two-line materials of the brassica napus; selecting radish varieties with white flowers; distant hybridization: carrying out distant hybridization in the early flowering period of recessive GMS line by adopting a two-time pollination method; harvesting hybrid seeds and carrying out bagging selfing onplants with fertile white flowers in hybrid F1; carrying out microspore culture on F2 to obtain a white flower strain material. The method disclosed by the invention has the advantages that bud-stripping time of a female parent is effectively shortened, the injury to stigma of the female parent is reduced and the influence of female parent pollen on hybrid offspring is eliminated, so that the success probability of the hybrid is improved; the white flower characters of radish are introduced into the brassica napus, and thereby a novel brassica napus material with white flowers is obtained; meanwhile, the varieties of ornamental rapeseed are enriched and the ornamental value is improved.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a method for breeding white-flowered rapeseed by hybridizing recessive male sterile lines of cabbage and rapeseed with radish. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Brassica napus (AACC, 2n=38) is an allotetraploid, derived from diploid cabbage. The natural doubling of Brassicarapa (AA, 2n=20) and diploid Brassica oleracea (CC, 2n=18) has a short evolutionary history and narrow genetic basis. To improve the yield, quality and resistance potential of Brassica napus varieties, it is urgent to expand the genetic basis of Brassica napus. Breeding Brassica napus varieties suitable for agricultural production and industrial use is the goal pursued by rapeseed breeders. Rapeseed breeders have always attached great importance to the material innovation and selection of Brassica na...

AI Technical Summary

Problems solved by technology

[0007] (1) The hybridization efficiency in the prior art is low, it is easy to produce false hybrids, and it is difficult to receive hybrid seeds;

[0008] (2) The amount of seeds received in the prior art is small, and the selection and breeding of hybrid offspring is difficult;

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