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Aspergillus echinosporus strain and application thereof

A technology of Aspergillus aculeatus and spores, which is applied to Aspergillus aculeatus strains and its application fields, can solve problems such as environmental safety, and achieve the effects of convenient use, high control efficiency, and simple use method

Active Publication Date: 2019-01-11
WUHAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects in the prior art, in order to solve the environmental safety problem in the chemical prevention and treatment of vegetable soil-borne diseases, to provide a kind of isolated Aspergillus aculeatus strain and its application in the prevention and control of soil-borne diseases

Method used

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  • Aspergillus echinosporus strain and application thereof
  • Aspergillus echinosporus strain and application thereof
  • Aspergillus echinosporus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The isolation and identification of embodiment 1 candidate bacterial strain

[0035] A. Collection of soil samples

[0036] In July 2017, in the plots where serious soil-borne diseases occurred in the Wuhu Base of Wuhan Academy of Agricultural Sciences in Hubei Province, China, the 5-point sampling method was used to take soil samples at 15-20cm below the ground to collect the rhizosphere of vegetable crops in facilities Soil, after natural air drying, save for later use;

[0037] B. Isolation and purification of candidate strains

[0038] Weigh 5g of soil sample and pour it into a conical flask, add sterile water to 50mL, shake at 28°C for 30min and let it stand still, which is 10 -1 Soil suspension; then take 1mL from the above solution, add 9mL sterile water and shake fully to make 10 -2 Soil dilution solution, draw 100μL dilution solution and apply on Martin's medium (formula: glucose 10g, peptone 5g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.5g, Bengal Red 0.03g, agar...

Embodiment 2

[0051] Example 2 Application of Aspergillus aculeatus Aa19 in the prevention and treatment of facility vegetable soil-borne disease cowpea wilt

[0052] The implementation steps are as follows:

[0053] A, Stability determination of Aspergillus aculeatus Aa19 strain

[0054] The screened Aspergillus aculeatus Aa19 was subcultured continuously for 10 generations on the PDA medium plate, and each generation was subcultured with cowpea wilt fungus JWS-1 (JWS-1 was sent to China. Wuhan. Wuhan on July 3, 2014. Preserved by the China Typical Culture Collection Center of the University, the preservation number is CCTCC NO: M 2014314, the strain has been authorized by the Chinese invention patent, the patent number is ZL 2014103340109, and the authorization announcement date is 2016.02.17) as an indicator bacterium, determined by the plate confrontation method Stability of antagonistic activity of Aspergillus aculeatus Aa19;

[0055] The results showed that the continuous 10-generat...

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Abstract

The invention belongs to the technical field of plant protection, in particular to an Aspergillus echinosporus strain and application thereof, and relates to biological control of soil-borne diseasesof vegetables. Aspergillus aculeatus (Aspergillus aculeatus) Aa19 is isolated from rhizosphere soil collected from vegetable plots where soil-borne diseases are serious. The strain is deposited in China Center for Type Culture Collection under the accession number CCTCC NO: M 2018648. Aspergillus aculeatus (Aspergillus aculeatus) Aa19 is isolated from the rhizosphere soil of vegetable plots wheresoil-borne diseases occurred. The stability of the strain of Aspergillus aculeatus, the activity of the spore suspension and the activity of the fermentation filtrate were tested by the reference number CWSC NO:M 2014314 for the Fusarium oxysporum JWS-1. The biological function verification proves that the isolate of the invention has good control effect on soil-borne diseases of vegetables. Aspergillus acanthosporus Aa 19 is safe to vegetables, has good stability and high bacteriostatic activity, and can be used for biological control of soil-borne diseases-cowpea wilt.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to an Aspergillus aculeatus strain and an application thereof. The invention relates to the field of biological control of vegetable soil-borne diseases. Background technique [0002] my country's facility vegetable industry is developing rapidly. In 2014, the national facility vegetable area exceeded 3.8 million hm 2 (57 million mu), the vegetable production methods have been further enriched, which is of great significance for ensuring the annual supply of vegetables and adjusting the structure of vegetable varieties. Compared with open field cultivation, the humidity in protected cultivation sheds is high, and it is difficult to rotate crops and stubble, which provides favorable conditions for the occurrence and prevalence of diseases and insect pests. The secondary salinization and acidification of the soil in the facilities, the imbalance of soil nutrients...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/66
CPCA01N63/30C12N1/145C12R2001/66
Inventor 吴仁锋申君杨绍丽叶安华陈峰
Owner WUHAN ACADEMY OF AGRI SCI
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