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Targeted magnetic nanoparticles with heating, chemotherapy and imaging functions, and preparation method and use thereof

A technology of nano-magnetism and magnetic nanoparticles, applied in the fields of biomedical engineering and nano-medicine, can solve the problems of poor curative effect and large toxic and side effects

Inactive Publication Date: 2019-01-11
JIANGSU CANCER HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, acute myelocytic leukemia (AML), whose incidence rate is increasing year by year, is a common hematological malignancy that seriously threatens the lives of young and middle-aged people in my country. , 100mg / bottle, produced by Pfizer Pharmaceutical Co., Ltd.) as the main drug in the chemotherapy regimen has the disadvantages of poor curative effect and large toxic and side effects

Method used

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  • Targeted magnetic nanoparticles with heating, chemotherapy and imaging functions, and preparation method and use thereof
  • Targeted magnetic nanoparticles with heating, chemotherapy and imaging functions, and preparation method and use thereof
  • Targeted magnetic nanoparticles with heating, chemotherapy and imaging functions, and preparation method and use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Use analytically pure FeCl 3 6H2O and FeCl 2 4H 2 O was dissolved in deionized water and prepared as 0.1mol / L FeCl 3 solution and FeCl 2 solution, by Fe 3+ :Fe2+ =5:3 (molar ratio) add 100ml of 0.1mol / L FeCl respectively 3 and 60ml of 0.1mol / L FeCl 2 solution in a flask under N 2 Add dropwise 1.5mol / L of NH under protection and magnetic stirring 4 OH, precipitates appear rapidly in the solution, continue to drop NH 4 OH until the pH of the reaction solution was 9, the precipitate gradually changed from reddish brown to black, and 1 g of oleic acid (oleic acid, OA) was added to continue stirring for 30 minutes, then stopped, and then matured in a water bath at 80°C for 60 minutes, and then held the bottom of the flask with a strong magnet. The waste liquid in the upper layer was poured off, the product was washed repeatedly with deionized water until the pH of the solution was 7, and the product was vacuum-dried for later use. Weigh the above 450mg oleic aci...

Embodiment 2

[0039] 1) Fe 3 o 4 Biocompatibility of @Ara-C nano drug delivery system

[0040] In vitro cytotoxicity is listed as grade 1; it does not cause hemolysis and has good blood compatibility; it has no genotoxicity to mouse bone marrow cells; the LD50 of Fe3O4@MTX nano drug delivery system after intraperitoneal injection of mice is determined to be 5.39g / kg , and its 95% CI is 3.58-7.72g / kg, which has a wide range of safe doses.

[0041] 2) In vitro evaluation of Fe by MRI 3 o 4 Marking effect of @Ara-C composite MNPs on AML cells

[0042] In vitro MRI showed that Fe 3 o 4 @Ara-C composite MNPs co-incubated with KG-1 cells at T 2 WI and T 2 *The signal intensity on WI was significantly reduced, indicating that Fe 3 o 4 @Ara-C Composite MNPs can enter KG-1 cells well, causing T 2 WI and T 2 *significantly decreased WI signal (see figure 2 ).

[0043] 3) In vitro comparison of Fe 3 o 4 @Ara-C Composite MNPs mediates the difference in curative effect between thermoche...

Embodiment 3

[0064] 1. Fe 3 o 4 @Ara-C composite nano-magnetic particles characterization method:

[0065] (1) Electron microscope morphology detection and component analysis: take a small amount of self-made Fe 3 o 4 @Ara-C composite nano-magnetic particles, add absolute ethanol and ultrasonically disperse for 15 minutes, drip with a film copper mesh, prepare electron microscope samples, and observe under H-600 TEM. Using any few fields of view under the transmission electron microscope, the components were identified by energy spectrometer analysis.

[0066] (2) Detection of average particle size and Zeta potential: take 1mg / mL Fe 3 o 4@Ara-C Composite nano-magnetic particle aqueous solution is placed in a cuvette, and the particle size and potential are measured with a ZETASIZER3000 laser particle size analyzer. The data is processed with dynamic light scattering software, and the average particle size and surface potential are recorded.

[0067] (3) X-Ray diffraction analysis (X-...

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Abstract

The invention discloses targeted magnetic nanoparticles with heating, chemotherapy and imaging functions, and a preparation method and use thereof. The targeted magnetic nanoparticles are drug-loadedmagnetic nanoparticles obtained by reacting an OA-Pluronic-stabilized Fe3O4 sol with an Ara-C solution; wherein the volume ratio of the OA-Pluronic-stabilized Fe3O4 sol to the Ara-C solution is 5- 10ml: 250-5000[mu]L. The Fe3O4 at Ara-C nanoparticles are excellent magnetic induction agents, chemotherapeutic agents and MRI contrast agents, which provide conditions for magnetic hyperthermia, chemotherapy, magnetic targeting and imaging drug tracing in vivo.

Description

technical field [0001] The invention belongs to the technical fields of biomedical engineering and nanomedicine, and specifically relates to a targeted nano-magnetic particle with functions of heating, chemotherapy and imaging, a preparation method and application thereof. Background technique [0002] Acute myeloid leukemia (AML) is a highly heterogeneous group of diseases, which can be transformed from malignant transformation of hematopoietic progenitor cells at different stages during the differentiation and development of normal myeloid cells, and originate from the transformation of progenitor cells at different stages. AML has distinct biological features. The latest risk analysis research report shows that since 1990, the incidence of AML has been increasing year by year, but the specific pathogenesis of AML is still not very clear. In the past forty years, the standard treatment for AML has not changed significantly. Except for acute promyelocytic leukemia, it is s...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/60A61K47/54A61K31/7068A61K41/00A61P35/02A61K49/18B82Y5/00B82Y15/00
CPCA61K9/0009A61K31/7068A61K41/0052A61K47/543A61K47/60A61K47/6929A61K49/1839A61P35/02B82Y5/00B82Y15/00A61K2300/00
Inventor 仲悦娇李云涛郭志睿孟庆奇彭钢张鹏季国忠戴昕妤葛瑶琪张艳沈富万田雪姚菁青
Owner JIANGSU CANCER HOSPITAL
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