Hydrogel compound, preparation method and application

A composite and hydrogel technology, which is applied in the field of biomedical engineering, can solve the problems of poor matrix integration, poorer performance than hyaline cartilage, and short cell life, and achieve the effects of rapid gelation, increased in vivo stability, and less trauma

Inactive Publication Date: 2019-01-08
JIANGSU DISSUETECH MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the cartilage regenerated by bone marrow stimulation is fibrocartilage, which is not as good as the original hyaline cartilage
And it will degenerate over time, and the transplantation of cartilage regenerated by bone marrow stimulation will have problems such as poor matrix integration, short cell life, and potential risk of disease metastasis

Method used

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  • Hydrogel compound, preparation method and application
  • Hydrogel compound, preparation method and application
  • Hydrogel compound, preparation method and application

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preparation example Construction

[0067] Preparation of aldehyde-modified hyaluronic acid

[0068] The aldehyde-modified hyaluronic acid of the present invention can be prepared by the following two methods:

[0069] The first method: oxidizing hyaluronic acid to open the ring to obtain aldehyde groups, and then obtain aldehyde group-modified hyaluronic acid (I). According to the molar ratio of HA to oxidizing agent (1:1)~(1:10), add oxidizing agent into hyaluronic acid solution (HA), and put the system in dark place to react for 0.5~6 hours. Ethylene glycol was added to remove excess oxidant, and after stirring was continued for 1 hour, the product was collected, purified and dried to obtain aldehyde-modified hyaluronic acid (I). The oxidizing agent may be one of periodate, permanganate, chlorate and the like.

[0070] The second method:

[0071] According to the above-mentioned first method to aldehyde-modified hyaluronic acid (I), on the basis of aldehyde-modified hyaluronic acid (I), carry out modif...

Embodiment 1

[0083] Sodium hyaluronate with a molecular weight of 100,000 to 200,000 Daltons was dissolved in water at room temperature to form a 0.01 g / mL hyaluronic acid solution. Add cation exchange resin and stir overnight, and filter to obtain hyaluronic acid solution (HA). Add 5mL of 0.5M sodium periodate solution to 100mL of 0.01g / mL hyaluronic acid solution for oxidation, and place the system in the dark for 3 hours to react. Add 0.5mL ethylene glycol to remove excess sodium periodate, continue stirring for 1 hour, collect the product, dialyze with a dialysis bag with a molecular weight cut-off of 3500 Daltons for three days, and freeze-dry to obtain aldehyde-modified hyaluronic acid (HA- A). The degree of substitution of the aldehyde group is 37% (such as figure 2 shown).

Embodiment 2

[0085] Sodium hyaluronate with a molecular weight of 100,000-200,000 Daltons is used to dissolve in water at room temperature to form a 0.03 g / mL hyaluronic acid solution. Add cation exchange resin and stir overnight, and filter to obtain hyaluronic acid solution (HA). Add 15 mL of 5M sodium periodate solution to 100 mL of 0.03 g / mL hyaluronic acid solution for oxidation, and place the system in the dark for 0.5 hours to react. Add 10 mL of ethylene glycol to remove excess sodium periodate, continue stirring for 1 hour, collect the product, dialyze for three days with a dialysis bag with a molecular weight cut-off of 3500 Daltons, and freeze-dry to obtain aldehyde-modified hyaluronic acid (HA-A ).

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Abstract

The invention provides a hydrogel compound, a preparation method and the application. The preparation method comprises the following steps: (1), preparing a hyaluronic acid solution; (2), preparing aldehyde-based modified hyaluronic acid; (3), preparing hydrazide derivatization hyaluronic acid; (4), mixing the aldehyde-based modified hyaluronic acid and the hydrazide derivatization hyaluronic acidinto hydrogel. The method ensures that a final product can be matched with a clinical arthroscopy on the basis of guaranteeing excellent biocompatibility through modification on hyaluronic acid, ensures that hydrogel is rapidly formed in any defective parts through one-time minimally invasive injection, can be further tightly adhered to the defective parts, so as to reduce burden of patients as far as possible, and achieve the effect of promotion of cartilage repair.

Description

technical field [0001] The invention relates to the field of biomedical engineering, and more specifically, to a hydrogel composite, a preparation method and an application. Background technique [0002] Hyaluronic acid is a non-sulfated glycosaminoglycan widely distributed in tissues. In recent years, hyaluronic acid has been used in many fields, such as in cosmetology, rheumatology, ophthalmology, pharmacy and surgery. In, especially for cartilage repair, osteoarthritis treatment, etc. [0003] Hyaluronic acid acts as a lubricant in the joint cavity and is also used to relieve the pain caused by arthritis. Due to its good biocompatibility, it is widely used in tissue repair. However, hyaluronic acid in its natural unmodified state is very easy to be quickly cleared in the body, so it is necessary to modify hyaluronic acid to prolong its half-life under the premise of ensuring biocompatibility. In the prior art, there are some materials (such as chitosan and sodium algin...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L5/08C08B37/08A61K9/06A61K47/36A61L27/26A61L27/52
CPCA61K9/06A61K47/36A61L27/26A61L27/52A61L2400/06C08B37/0072C08J3/075C08J2305/08C08J2405/08C08L5/08
Inventor 葛子钢阳佳蓓陈佳青朱健万文倩
Owner JIANGSU DISSUETECH MEDICAL TECH CO LTD
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