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A spectrophotometric multi-wavelength detection device and detection method thereof

A spectrophotometric and detection device technology, applied in the field of spectral analysis, can solve the problems of restricting the application and promotion of an array system, high manufacturing difficulty and cost, and inability to match absorption cells, so as to reduce the manufacturing difficulty and cost, and reduce the surface roughness. Requirements and the effect of ensuring measurement reliability

Active Publication Date: 2020-05-19
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, multiple absorption cells of this type of array chip are integrated on the chip, and the absorption cells cannot be paired
This leads to a system with a complex structure, especially a system with a complex structure, which is extremely difficult and costly to manufacture, and the more channels, the higher the difficulty and cost
This seriously restricts the application and promotion of this type of array system

Method used

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  • A spectrophotometric multi-wavelength detection device and detection method thereof
  • A spectrophotometric multi-wavelength detection device and detection method thereof
  • A spectrophotometric multi-wavelength detection device and detection method thereof

Examples

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Embodiment 1

[0070] reference Figure 4 to Figure 5 As shown, the embodiment of the present invention provides a spectrophotometric multi-wavelength detection device 100 and a detection method.

[0071] The spectrophotometric multi-wavelength detection method provided in this embodiment includes the following steps:

[0072] S1, express the influence of the surface roughness of the absorption cell in the form of absorbance. According to the additivity of absorbance, combine with the absorption of multiple components to construct a multi-wavelength correction model that includes the influence of surface scattering; specifically, it can be:

[0073] Considering the influence of the non-ideal rough surface of the absorption cell, the surface is regarded as a component of the multi-component, and the multi-material absorption is combined to obtain the multi-component multi-wavelength calibration model:

[0074]

[0075] Using vectors to represent different columns in the matrix, the above matrix can be...

Embodiment 2

[0094] In order to specifically illustrate the detection process and effects of the present invention, the concentration detection of different concentrations of orange G solution in 11 PMMA cuvettes with different surface roughness is taken as an example. The spectrum and single-point absorbance measurement in the whole example are in Lambda 850 (PerkinElmer).

[0095] The 13 cuvettes used in the example are figure 1 Shown as figure 2 The cuvette shown in has a surface 1 and a surface 2 with different roughness. The incident light is emitted from the surface 2 through the surface 1 and the sample (different concentrations of orange G solution) to form transmitted light. The two surfaces of No. 1-10 cuvettes have been polished to different degrees and have a large difference in surface roughness, which is used to illustrate the elimination effect of the method of the present invention on the difference in surface roughness. Table 1 shows the transmittance of deionized water in t...

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Abstract

The invention discloses a spectrophotometric multi-wavelength detection device and a detection method. The spectrophotometric multi-wavelength detection device includes: a multi-wavelength correction model building module, which constructs a multi-wavelength correction model including the influence of surface scattering; a wavelength selection module, which selects within the wavelength range where one or more absorption peaks of the target substance to be measured are located. wavelength; an absorption spectrum measurement module, which measures the absorption spectrum relative to air; a calculation module, which obtains the concentration measurement result of the target substance to be measured through calculation according to the multi-wavelength correction model. The spectrophotometric multi-wavelength detection device and detection method provided by the invention can effectively eliminate the influence of the difference in the surface roughness of the absorption cell on the spectrophotometric analysis, and can greatly reduce the surface roughness requirement of the absorption cell.

Description

Technical field [0001] The present invention relates to the technical field of spectral analysis, in particular to a spectrophotometric multi-wavelength detection device and a detection method thereof. Background technique [0002] When spectrophotometry is used in the quantitative analysis of the target in the solution, in order to ensure that the result is not affected by the absorption cell, use the same absorption cell to hold the sample and the reference solution separately or use two absorption cells with the same transmittance after paired blanks Hold the sample and reference solution. [0003] For some array-type multi-channel micro-absorption cells, such as centrifugal multi-channel microfluidic chips, the absorption cell can only be injected once, and the sample and reference solution can only be stored in two different absorption cells. At the same time, multiple absorption cells of this type of array chip are integrated on the chip, and the absorption cells cannot be m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/01G01N21/31
CPCG01N21/01G01N21/274G01N21/314G01N2021/0112G01N2201/127
Inventor 吴一辉徐阳迟明波孙传盛
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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