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Antibody purifying method

An antibody purification and antigen technology, applied in chemical instruments and methods, peptide preparation methods, organic chemistry, etc., can solve problems such as human injury, and achieve the effects of low production cost, high antigen titer, and easy operation.

Inactive Publication Date: 2019-01-04
ELABSCI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Residual host cell proteins may stimulate the body to generate an immune response to produce corresponding antibodies, which will also cause harm to the human body

Method used

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Embodiment Construction

[0020] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention , but not all examples. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0021] Influenza virus is an important seasonal infectious disease. Several major outbreaks in history have caused huge damage and loss to human health and economy. Since the discovery of influenza virus, people have devoted themselves to the diagnosis, prevention and control of influenza virus. Influenza A virus has the characteristics of high variability and numerous subt...

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Abstract

An embodiment of the invention provides an antibody purifying method. Animals immunized by using M1 recombinant protein have higher reactivity to recombinant protein than natural protein, and a monoclonal antibody stable in performance and high in antigen valence can be generated; a CA-AS method is adopted for antibody purifying, so that great advantages exist in the aspects of antibody recovery rate, antibody activity and antibody purity. The antibody purifying method is simple and convenient in operation, reusable and low in production cost.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of biopharmaceuticals, and more specifically, to an antibody purification method. Background technique [0002] The process research and development of contemporary antibody drugs is based on genetic engineering, cell engineering and fermentation engineering. Chinese hamster ovary (CHO) cells, as the main mammalian cell expression host cells, have the characteristics of clear genetic background and post-translational processing capabilities. It has become a standard industrialized cell line for protein production and is also suitable for the expression of antibody proteins. With the development of cell suspension culture technology, large-scale culture is possible, and the application of serum-free medium makes the protein purification process more convenient, all of which greatly accelerate the industrialization process of antibody drugs, and provide a new way for the preparation o...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K1/36C07K1/30C07K1/18
CPCC07K1/18C07K1/30C07K1/36C07K16/1018
Inventor 冷毅斌王长乐
Owner ELABSCI BIOTECH
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