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Stephania epigaea tissue culture reproduction method

A tissue culture and proliferation culture technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of intolerant small leaves, long germination cycle, large variability, etc. The effect of low production cost

Inactive Publication Date: 2019-01-04
SOUTHWEST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the development of medicinal value and the promotion of ornamental value, the demand for land tolerance has increased by leaps and bounds, and the limited wild resources cannot meet the market demand, and its price has also risen rapidly. Many scientific research institutions and enterprises have Carry out planting technology research on incompatible land, but because the land does not allow dioecious plants, and the distribution of female plants in the population is small, the number of seeds is small, and at the same time, due to the low seed germination rate and long germination cycle
At the same time, because it is a dioecious plant, the hybridization of seeds is strong, and as an ornamental plant, its variability is large, and the genetic traits of the female parent are unstable.
In recent years, some units have carried out research on tissue culture techniques such as Guangxi ground tolerance and lobular ground tolerance, but the results are not satisfactory in terms of proliferation rate and rooting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Plant the strong tubers without stems and leaves indoors. After the new stems and leaves are sent out, spray 2 times with 75% carbendazim 800 times solution every 4 days, and select the young stems with buds;

[0020] (2) Cut the selected explants into small sections with buds, rinse with saturated soapy water for 10 minutes, rinse with tap water for 3-4 times until there is no soapy water residue, and rinse with tap water for 60-70 minutes;

[0021] (3) Transfer the cleaned explants to the ultra-clean workbench, disinfect with 75% alcohol for 10 seconds, rinse with sterile water for 2-3 times, disinfect with saturated bleach solution for 20 minutes, rinse with sterile water for 3-4 times , 0.05% mercuric solution + 2-3 drops of Tween-80 for 10 minutes, rinse with sterile water 6-8 times;

[0022] (4) Use sterile filter paper to absorb the surface moisture of the sterilized explants, remove both ends and cut them into 0.5-1.0cm bud stem segments, insert them oblique...

Embodiment 2

[0026] (1) Plant the strong tubers without stems and leaves indoors. After the new stems and leaves are sent out, spray 3 times with 75% carbendazim 800 times solution every 5 days, and select the young stems with buds;

[0027] (2) Cut the selected explants into small sections with buds, rinse with saturated soapy water for 10 minutes, rinse with tap water for 3-4 times until there is no soapy water residue, and rinse with tap water for 60 minutes;

[0028] (3) Transfer the cleaned explants to the ultra-clean workbench, disinfect with 75% alcohol for 10 seconds, rinse with sterile water for 2-3 times, disinfect with saturated bleach solution for 20 minutes, rinse with sterile water for 3-4 times , 0.05% mercuric solution + 2-3 drops of Tween-80 for 15 minutes, rinse with sterile water 6-8 times;

[0029] (4) Use sterile filter paper to absorb the surface moisture of the sterilized explants, remove both ends and cut them into 0.5-1.0cm bud stem segments, insert them obliquely ...

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PUM

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Abstract

The invention discloses a stephania epigaea tissue culture reproduction method. The stephania epigaea tissue culture reproduction method comprises the following steps: (1) explant selection, (2) explant sterilization, (3) cluster bud induction, (4) multiplication culture, (5) rooting culture and the like. A stephania epigaea tissue culture rapid propagation system is established successfully, andstephania epigaea cluster bud induction rate, multiplication factors and the rooting rate are improved remarkably. The stephania epigaea tissue culture reproduction method has the advantages of high tissue culture efficiency and low cost, and the problems that wild stephania epigaea resources are developed excessively, medicinal material supply is less, a planting scale is limited by the number and quality of seedlings and the like are solved.

Description

technical field [0001] The invention relates to the field of tissue culture of Chinese herbal medicines, in particular to a method for tissue culture and propagation of groundwater. Background technique [0002] Dirong (Stephania epigaea H.S.Lo) is a herbaceous vine of the genus Stephaniaceae. Its roots are used as medicine. It has the effects of expelling wind and dampness, clearing away heat and detoxification, and relieving pain. It is mainly used for stomach pain, rheumatic arthralgia, unfavorable throat, and carbuncle sores. It contains Luotongding, which has antipyretic and analgesic effects, and is mainly used to treat gastric ulcer, duodenal ulcer pain, menstrual pain, postpartum uterine contraction pain, tension insomnia, and spasmodic cough in clinical practice; Containing sinomenine has analgesic and anti-inflammatory effects, and is mainly used to treat rheumatoid arthritis and neuralgia. At the same time, due to the strange and peculiar properties of its tuber...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 邓国宾张媛赵露琴向建英杨燕曾祥飞曾琼
Owner SOUTHWEST FORESTRY UNIVERSITY
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