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Application of bacillus in promoting growth of plants

A technology for promoting plant growth and Bacillus, applied in the field of microorganisms, can solve problems such as yellowing of dwarf plants, low yield, and poor quality, and achieve the effects of reducing the use of chemical fertilizers, promoting plant growth, and reducing fermentation costs.

Pending Publication Date: 2018-12-28
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lack of any element will cause the metabolism and photosynthesis of plants to be disturbed and inhibited, resulting in short plants, yellow leaves, low yield and poor quality

Method used

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  • Application of bacillus in promoting growth of plants
  • Application of bacillus in promoting growth of plants
  • Application of bacillus in promoting growth of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Classification and identification of bacillus strains

[0026] Bacillus HTTA-X0189 strain, in solid peptone medium: peptone 4g, yeast powder 2g, NaCl, 15-25g, KCl 0.2-0.4g, MgCl 2 ·7H 2 O 1.5-2.5g, water 1000ml, pH 7.0, agar 16-19g, culture at 25°C-27°C for 2-3 days, observe and record changes in colony shape, color, etc., observe the shape of bacteria with a microscope, and test their physiology by conventional methods for bacterial identification biochemical reaction. Bacterial strains were cultivated by the above-mentioned method, using the general primers of bacterial 16SrDNA gene, sequencing of PCR amplification products by conventional method, and comparing by NCBI BLAST program, analyzing sequence homology and making phylogenetic branch tree (see figure 1 ) are relatively closely related species.

[0027] The results proved that the surface of Bacillus tequila HTTA-X0189 colony with normal growth was rough and opaque, the colony was white at the ini...

Embodiment 2

[0032] Example 2: Determination of the relevant genes and effects of nitrogen fixation and phosphorus and potassium degradation of bacterial strains

[0033] According to the principle that various functions of microorganisms are controlled by genes, first find the general primers of nitrogen fixation reductase nifH gene and phytase phy gene through NCBI, use the genomic DNA of the strain as a template, and perform PCR amplification by conventional methods to detect the strain Whether it has the ability to fix nitrogen and degrade macromolecular organic phosphorus, and at the same time pass the reported test microbial nitrogen fixation medium (ACC medium), potassium degradation medium (MYK medium), inorganic phosphorus degradation medium (PKO culture medium) Base) and other culture strains and observe their growth conditions to determine their function with genetic testing.

[0034] The result proves that the size of the nifH gene fragment amplified to the bacterial strain by ...

Embodiment 3

[0036] Example 3: Production of siderophores, hormones and related genes by strains

[0037] By the same specific primer amplification method as in Example 1, first check the ysnE and yhcX genes of the HTTA-X0189 strain to synthesize the plant hormone indole acetic acid IAA and the alsS, alsD, and alsR genes associated with promoting plant stress resistance, and then pass Biological methods such as culture tests are verified, and the reported test microorganisms produce the R of L-tryptophan of indole acetic acid IAA 2 A culture medium, CAS culture medium whether to produce siderophore, observe the growth state, measure the absorbance value (630nm) As (0.2<As<0.8) of bacterial liquid treatment, and calculate siderophore unit activity, siderophore activity %=[(Ar -As) / Ar] x 100%. 3-1

[0038] The results proved that the ysnE and yhcX gene fragments were amplified from the genome of the Bacillus tequila HTTA-X0189 strain, and the sizes were 1260bp and 500bp respectively to con...

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Abstract

The invention discloses application of bacillus in promoting growth of plants. The bacillus is Bacillus tequilensis, the bacterial strain number of which is HTTA-X0189, and the preservation number ofwhich is CGMCC No.15770. The HTTA-X0189 bacterial strain is stable in biological characteristic and fertilizer action, simple and feasible in culture method, low in production cost and easy to realizeindustrialization. The HTTA-X0189 bacterial strain can promote growth of plants from multiple aspects, further can induce salt resistance of the plants, and is the bacterial strain which plays a multifunctional promoting role to the plants, and use of chemical fertilizers can be reduced.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to the application of a bacillus in promoting plant growth. Background technique [0002] Cultivation of plants is the guarantee for the survival of human beings. In our country, there are few lands and many people. The use of fertilizers is an important measure to increase the yield per unit area of ​​crops. The physical and chemical properties of the crops are gradually deteriorating, the soil is acidified and hardened, the normal structure is destroyed, and the soil fertility declines. Cellulose is the most widely distributed resource on the earth, but other organisms, including plants, cannot directly use resources. In my country, only agricultural waste can be as high as 0.7×10 per year. 9 tons, most of these raw materials are incinerated, which not only has a low utilization rate, but also brings enormous pressure to environmental protection. Microorganisms can degrad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C05G3/00C05F11/08C12R1/07
CPCA01N63/00C05B13/02C12N1/205C12R2001/07Y02P60/21
Inventor 陈杰袁静黄晓慧
Owner ZHEJIANG FORESTRY UNIVERSITY
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