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Standardized high-throughput three-dimensional cell preparation device and application thereof

A technology of three-dimensional cells and preparation devices, which is applied in the direction of tissue cell/virus culture devices, measurement devices, biochemical equipment and methods, etc., which can solve the problem of combining patterned microwells with commercial well plates and limiting the promotion and use of microwell methods , unable to carry out standardized operations and other problems, to achieve easy-to-achieve effects

Inactive Publication Date: 2018-12-28
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires special equipment and can only be carried out in a small number of qualified laboratories. In addition, the patterned microwells prepared by photolithography and other techniques cannot be combined with commercial well plates, and standardized operations cannot be performed.
These problems greatly limit the promotion and use of the microporous method

Method used

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  • Standardized high-throughput three-dimensional cell preparation device and application thereof
  • Standardized high-throughput three-dimensional cell preparation device and application thereof
  • Standardized high-throughput three-dimensional cell preparation device and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Such as figure 1 The standardized high-throughput three-dimensional cell preparation device shown in a, in a commercial 96-well plate, has an array of concave structures at the bottom. One of the fabrication methods is as follows. Firstly, a PDMS chip with an array of recessed structures is fabricated into a circular chip with a diameter of 6 mm. The concave structure on the chip is an inverted trapezoidal structure, the diameter of the upper part is 600 μm, and the diameter of the lower part is 300 μm. The chip was fixed at the bottom of a commercial 96-well plate, and the effect was as follows: figure 1 as shown in b.

[0036] The bottom of the well plate was treated with 0.2% PF-127 for 4 hours and rinsed three times with PBS. Caco-2, U87, HepG2 cells were digested and resuspended in cell culture medium at 3×10 5 The density of cells / well was inoculated into each well respectively, and cultured in a cell culture incubator. After 24 hours, three-dimensional cell ...

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Abstract

The invention provides a standardized high-throughput three-dimensional cell preparation device and application thereof, especially for the construction of a three-dimensional cell model. According tothe provided standardized high-throughput three-dimensional cell preparation device, a concave structure is arranged on the bottom of a commercialized orifice plate, after cells are inoculated, due to the bottom concave structure, the cells are aggregated into a three-dimensional cell cluster, and the device can be used for the cultivation of three-dimensional cells, drug evaluation and other research. The three-dimensional cells prepared by the device are controllable in size and convenient to use, and can be standardized for subsequent operation. The standardized high-throughput three-dimensional cell preparation device and the application thereof solve the problem that the preparation of the three-dimensional cells cannot be combined with the orifice plate capable of being operated conveniently and quickly, the standardization and high throughput are not easy, and the detection cannot be carried out rapidly, and facilitate the follow-up study.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a standardized high-throughput three-dimensional cell preparation device and its application. Background technique [0002] As we all know, the vast majority of cells in the human body live in a three-dimensional environment. Cells must obtain various biochemical and mechanical signals through cell-cell and cell-extracellular matrix interactions in order to maintain their normal physiological activities. A large number of studies have shown that the interaction between cells and extracellular matrix and between cells plays an important role in regulating cell migration, proliferation and differentiation. The existing two-dimensional monolayer cell culture cannot truly reflect the living environment of cells in vivo, lacks such signal transmission, and cannot well simulate the microenvironment of cells in vivo, which is very different from that in vivo. An insurmountable gap. ...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12Q1/02
CPCC12M23/12G01N33/5008
Inventor 秦建华李中玉魏文博
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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