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A kind of highly water-soluble porcine small intestine digestive enzyme powder and its preparation method and application

A technology of digestive enzyme powder and digestive enzyme powder, applied in the field of highly water-soluble porcine small intestine digestive enzyme powder and its preparation, can solve the problems of high loss of digestive enzyme activity, low enzyme specific activity, low water solubility, etc. , Promote dissolution and improve recovery

Active Publication Date: 2021-01-15
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of the invention can solve the disadvantages of high loss of digestive enzyme activity and low specific activity of the enzyme in the traditional preparation process, and overcome the problem of low water solubility of the enzyme powder when used

Method used

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  • A kind of highly water-soluble porcine small intestine digestive enzyme powder and its preparation method and application
  • A kind of highly water-soluble porcine small intestine digestive enzyme powder and its preparation method and application
  • A kind of highly water-soluble porcine small intestine digestive enzyme powder and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, prepare highly water-soluble small intestine digestive enzyme powder with porcine small intestine fluid

[0050] 1.1 Collection of porcine small intestinal fluid

[0051] Twelve castrated boars with similar genetic background, similar parity, and body weight of 18.05±1.57kg (mean±standard deviation) were selected and raised in single cages in metabolic cages. After 1 week of adaptation, a T-shaped fistula was installed at the front of the small intestine, and the pigs were reared in metabolic cages for 3 weeks after the operation. Centrifuge the millet (centrifugation conditions: 4-8°C, 9000-11000g, centrifuge for 10-15 minutes), collect the supernatant 1 (that is, the small intestinal fluid); mix 40L of the small intestinal fluid evenly, and then pack it into a 1L plastic bottle Stored in medium for later use, and samples were taken to measure the activities of trypsin, chymotrypsin and amylase in intestinal fluid after mixing. During the period of dige...

Embodiment 2

[0074] Example 2, the effect of preparing simulated small intestine fluid based on pig small intestine fluid purification powder

[0075] 2.1 Preparation of highly water-soluble porcine small intestine digestive enzyme purified powder

[0076] The preparation method of the small intestine digestive enzyme purified powder is the same as that in Example 1.

[0077] 2.2 Preparation of small intestinal fluid and simulated small intestinal fluid in pigs

[0078] The preparation of the small intestinal fluid in pigs is the same as the supernatant 1 in Example 1, and the activity determination of the main digestive enzymes in the small intestinal fluid is shown in Example 1. The preparation of simulated small intestine fluid is as follows: according to the digestive enzyme with the largest specific activity ratio of amylase, trypsin and chymotrypsin in the purified powder of small intestine digestive enzymes relative to the corresponding digestive enzyme activity in supernatant 1, a...

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Abstract

The invention discloses a highly water-soluble porcine small intestine digestive enzyme powder and a preparation method thereof. The pig small intestine digestive enzyme powder is prepared according to a method comprising the following steps: 1) collecting intestinal chyme from the small intestines of several pigs and mixing them uniformly, and then centrifuging the mixed intestinal chyme 1. Collect the supernatant 1; 2) Evaporate and concentrate the supernatant 1 to obtain concentrated intestinal juice; centrifuge the concentrated intestinal juice 2 to collect the supernatant 2; 3) Transfer the supernatant 2 Put it in a container placed in an ice-water bath, and add mannitol therein, after stirring and dissolving, then add ethanol solution, stir evenly and let it stand; then carry out centrifugation 3, collect the precipitate; 4) add deionized water to the precipitate , and stirred and ultrasonically dissolved, and then centrifuged 4 to collect the supernatant 3; the supernatant 3 was freeze-dried to obtain. The intestinal digestive enzyme powder prepared by the invention improves the specific activity of the digestive enzyme, reduces the impurity content and improves the solubility of the digestive enzyme.

Description

technical field [0001] The invention relates to a highly water-soluble porcine small intestine digestive enzyme powder and its preparation method and application. Background technique [0002] In the field of feed production, the procurement of feed raw materials and the optimization of feed formulations are very dependent on the method of objectively evaluating the potency of feed nutrients (such as energy, amino acids) or the effective content of functional components under laboratory conditions. For this reason, scholars from various countries try to establish a technology for quickly evaluating the effective nutrients of feed by simulating the digestion of feed in animals. In this technology, the digestive enzyme reagent (or preparation) is the most critical material, which determines the simulation effect of the digestive environment in the body, thereby affecting the objective evaluation of the nutritional value of the feed. At the same time, in the simulated digestio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00
CPCG01N33/00
Inventor 赵峰党方昆张虎于耀杜中原
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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