Preparation method of livestock feed premix as well as product and application thereof
A premix and feed technology, applied in the field of livestock and poultry feed, can solve problems such as growth obstacles, and achieve the effects of promoting recovery, promoting protein digestion, and improving daily weight gain.
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Embodiment 1
[0030] Embodiment 1, the influence of calcium glycinate on piglet weaning diarrhea and production performance
[0031] A total of 120 weaned piglets weighing close to 5kg and 21 days old were selected and randomly divided into 4 groups according to the test requirements. Each group had 3 replicates, and each replicate had 10 piglets. Drink water freely, calculate the daily feed intake every day, observe and record the diarrhea situation every morning, weigh once every 10 days, and record the feed intake of each repetition. Then the diarrhea rate, daily weight gain and feed-to-weight ratio were counted.
[0032] Among them, diarrhea rate = number of pigs with diarrhea / total number of pigs × 100%, the result is as follows figure 1 shown.
[0033] It can be seen from the figure that there is a relatively high proportion of diarrhea in each group at the age of 7-9 days after weaning, and the diarrhea rate of each test group decreased significantly after the 9th day, and the diar...
Embodiment 2
[0039] Embodiment 2, Calcium Glycinate Effects on Weaning Piglet Mesenteric Lymph Node Lymphocyte Proliferation
[0040] Determination of lymphocyte proliferation in mesenteric lymph nodes requires slaughtering of animals. The specific method is as follows: slaughtering is divided into 3 times, and 3 21-day-old suckling piglets are taken for the first time, and serum is collected from slaughtering. Samples of duodenum, jejunum, and ileum are taken as unweaned previous comparison. Then 10 days after weaning and 20 days after weaning, 6 heads were taken from each group, slaughtered and sampled. The experimental pigs were fasted for 24 hours under free access to water before slaughter.
[0041] (1) Tissue sampling and processing
[0042] Carefully dissect the abdominal cavity, cut out the mesenteric lymph nodes in the middle of the jejunum with sterile scissors, and put them into a sterile bottle dish, which is first added with an appropriate amount of sterile PBS. Transfer to...
Embodiment 3
[0053] Example 3, Effects of calcium glycinate on villi height and lamina propria thickness of each intestinal segment
[0054] Making slices and measuring villous height (VH) and lamina propria deph (LPD):
[0055] (1) Take 1 cm from the middle section of duodenum, jejunum and ileum respectively 2 The intestinal wall was picked up carefully with tweezers and rinsed with saline, pasted on filter paper, flattened and trimmed, and fixed in 4% formalin.
[0056] (2) Rinse: Take out the fixed tissue block and trim it, put it into a large beaker and fill it with water, tie the mouth with gauze, and rinse with running water for 24 hours;
[0057] (3) Dehydration, transparency and wax dipping: the rinsed tissues were dehydrated on a Leica TP1020 dehydrator, transparent and wax dipped.
[0058] (4) Embedding, slicing, patching, displaying, and baking slices were embedded in the Leica EG1160 tissue embedding instrument; the embedding box with the tissue block was fixed on the stage o...
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