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Piglet intestinal health regulation and control bioactive peptide fodder additive and application thereof

A feed additive and intestinal health technology, which is applied in piglet intestinal health regulation active peptide feed additives and application fields, can solve problems such as insufficient digestive enzyme activity, low feed utilization rate, immature immune function, etc., and achieve significant economic benefits and social benefits, reducing the rate of weaning diarrhea, and improving the effect of average daily gain

Pending Publication Date: 2020-03-17
BRIGHT (HANGZHOU) FEED SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, an important problem brought about by early weaning is how to improve the growth performance of weaned piglets. After weaning, the nature of the diet of piglets has undergone major changes, and the digestive tract of piglets has not yet fully developed, the activity of digestive enzymes is insufficient, and the immune function has not yet matured. Moreover, weaning is not only related to physiological changes, but also changes in psychology, environment and nutritional status, so it often manifests as poor appetite, digestive disorders, diarrhea, growth retardation, and low feed utilization. The so-called "piglet early weaning syndrome"

Method used

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  • Piglet intestinal health regulation and control bioactive peptide fodder additive and application thereof
  • Piglet intestinal health regulation and control bioactive peptide fodder additive and application thereof
  • Piglet intestinal health regulation and control bioactive peptide fodder additive and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of koji for strains

[0024] (1) Preparation of Bacillus subtilis seed liquid

[0025] Bacillus subtilis (Bacillus subtilis) ACCC01185 (purchased from the China Agricultural Microorganism Culture Collection Management Center) was inoculated into the slant medium, and cultured at 37°C for 24 hours to obtain the slant strain;

[0026] Each 300ml triangular bottle contains 50ml of seed culture medium, sterilized at 120°C for 30 minutes, after cooling down, connect a ring of bacterial lawn to the seed culture medium from the inclined surface, and cultivate it on a temperature-controlled shaker at 37°C with a speed of 220r / min for 24 hours. It is Bacillus subtilis seed solution.

[0027] The composition of the slant medium is: peptone 1g, beef extract 0.3g, NaCl 0.5g, agar 2.0g, distilled water 100ml, pH value 7.0.

[0028] The composition of the seed medium is: glucose 0.2g, NaCl 0.5g, yeast extract 0.5g, peptone 1g, distilled water 100mL, pH 7.0.

[0029]...

Embodiment 2

[0044] 1. Preparation of koji for strains

[0045] (1) Bacillus subtilis seed liquid:

[0046] Inoculate Bacillus subtilis (Bacillus subtilis) ACCC01185 (purchased from China Agricultural Microorganism Culture Collection and Management Center) into the slant medium, and cultivate it at 37°C for 24h to obtain the slant strain; Sterilize under the same conditions for 30 minutes, after cooling down, connect a ring of bacterial lawn from the inclined surface to the seed medium, place it on a temperature-controlled shaker at 35°C, and cultivate it at a speed of 220r / min for 26 hours to obtain the Bacillus subtilis seed liquid.

[0047] The slant medium is: peptone 1g, beef extract 0.3g, NaCl 0.5g, agar 2.0g, distilled water 100ml, pH 7.0.

[0048] The seed medium is: glucose 0.2g, NaCl 0.5g, yeast extract 0.5g, peptone 1g, distilled water 100mL, pH7.0.

[0049] (2) Saccharomyces cerevisiae seed solution

[0050] Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20042 (purc...

Embodiment 3

[0063] 1. Preparation of koji for strains

[0064] (1) Bacillus subtilis seed liquid

[0065] Bacillus subtilis (Bacillus subtilis) ACCC01185 (purchased from the China Agricultural Microorganism Culture Collection Management Center) was inoculated into the slant medium, and cultured at 37°C for 24 hours to obtain the slant strain;

[0066] Each 300ml triangular bottle contains 50ml of seed medium, sterilized at 120°C for 30 minutes, and after cooling down, connect a ring of bacterial lawn to the seed medium from the slope, and cultivate it on a temperature-controlled shaker at 36°C with a speed of 220r / min for 25 hours. It is Bacillus subtilis seed solution.

[0067] The slant medium is: peptone 1g, beef extract 0.3g, NaCl 0.5g, agar 2.0g, distilled water 100ml, pH 7.0.

[0068] The seed medium is: glucose 0.2g, NaCl 0.5g, yeast extract 0.5g, peptone 1g, distilled water 100mL, pH7.0.

[0069] (2) Yeast seed liquid

[0070] Saccharomyces cerevisiae (Saccharomyces cerevisiae...

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PUM

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Abstract

The invention discloses piglet intestinal health regulation and control bioactive peptide fodder additive and application thereof. The active peptide fodder additive is prepared by the following steps: mixing vital gluten and silkworm chrysalis meal, adding water, and carrying out uniformly stirring; adding papain and trypsin, adjusting the pH value to 7.0 to 8.0, and carrying out enzymolysis at atemperature of 50 to 55 DEG C for 30 to 60 minutes to obtain an zymolyte; inoculating bacillus subtilis and saccharomyces cerevisiae into a solid-state fermentation culture medium prepared by the zymolyte, carrying out blending fermentation culturing for 30 to 45 hours at a temperature of 33 to 37 DEG C, and drying the fermentation mixture to obtain the bioactive peptide fodder additive. With theactive peptide, intestinal mucosa villi can be prevented from excessively shrinking and recessing deepening can be prevented 1-7 days after weaning of piglets and the intestinal mucosa villi height can be remarkably increased 8 to 14 days after weaning.

Description

[0001] (1) Technical field [0002] The invention relates to an active peptide feed additive for regulating intestinal health of piglets, a preparation method and application thereof. [0003] (2) Background technology [0004] Early weaning can improve the fertility of sows, increase the annual litter size of sows, reduce the transmission of diseases from mothers to piglets, and improve the production performance and carcass quality of growing pigs, and at the same time increase the utilization rate of equipment such as delivery rooms . However, an important problem brought about by early weaning is how to improve the growth performance of weaned piglets. After weaning, the nature of the diet of piglets has undergone major changes, and the digestive tract of piglets has not yet fully developed, the activity of digestive enzymes is insufficient, and the immune function has not yet matured. Moreover, weaning is not only related to physiological changes, but also changes in psyc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/14A23K10/12A23K20/147A23K50/30A23K50/60
CPCA23K10/14A23K10/12A23K20/147A23K50/30A23K50/60
Inventor 徐求文金妙仁楼伟杰于晓莹洪奇华
Owner BRIGHT (HANGZHOU) FEED SCI TECH CO LTD
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