Piglet intestinal health regulation and control bioactive peptide fodder additive and application thereof
A feed additive and intestinal health technology, which is applied in piglet intestinal health regulation active peptide feed additives and application fields, can solve problems such as insufficient digestive enzyme activity, low feed utilization rate, immature immune function, etc., and achieve significant economic benefits and social benefits, reducing the rate of weaning diarrhea, and improving the effect of average daily gain
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Embodiment 1
[0023] 1. Preparation of koji for strains
[0024] (1) Preparation of Bacillus subtilis seed liquid
[0025] Bacillus subtilis (Bacillus subtilis) ACCC01185 (purchased from the China Agricultural Microorganism Culture Collection Management Center) was inoculated into the slant medium, and cultured at 37°C for 24 hours to obtain the slant strain;
[0026] Each 300ml triangular bottle contains 50ml of seed culture medium, sterilized at 120°C for 30 minutes, after cooling down, connect a ring of bacterial lawn to the seed culture medium from the inclined surface, and cultivate it on a temperature-controlled shaker at 37°C with a speed of 220r / min for 24 hours. It is Bacillus subtilis seed solution.
[0027] The composition of the slant medium is: peptone 1g, beef extract 0.3g, NaCl 0.5g, agar 2.0g, distilled water 100ml, pH value 7.0.
[0028] The composition of the seed medium is: glucose 0.2g, NaCl 0.5g, yeast extract 0.5g, peptone 1g, distilled water 100mL, pH 7.0.
[0029]...
Embodiment 2
[0044] 1. Preparation of koji for strains
[0045] (1) Bacillus subtilis seed liquid:
[0046] Inoculate Bacillus subtilis (Bacillus subtilis) ACCC01185 (purchased from China Agricultural Microorganism Culture Collection and Management Center) into the slant medium, and cultivate it at 37°C for 24h to obtain the slant strain; Sterilize under the same conditions for 30 minutes, after cooling down, connect a ring of bacterial lawn from the inclined surface to the seed medium, place it on a temperature-controlled shaker at 35°C, and cultivate it at a speed of 220r / min for 26 hours to obtain the Bacillus subtilis seed liquid.
[0047] The slant medium is: peptone 1g, beef extract 0.3g, NaCl 0.5g, agar 2.0g, distilled water 100ml, pH 7.0.
[0048] The seed medium is: glucose 0.2g, NaCl 0.5g, yeast extract 0.5g, peptone 1g, distilled water 100mL, pH7.0.
[0049] (2) Saccharomyces cerevisiae seed solution
[0050] Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20042 (purc...
Embodiment 3
[0063] 1. Preparation of koji for strains
[0064] (1) Bacillus subtilis seed liquid
[0065] Bacillus subtilis (Bacillus subtilis) ACCC01185 (purchased from the China Agricultural Microorganism Culture Collection Management Center) was inoculated into the slant medium, and cultured at 37°C for 24 hours to obtain the slant strain;
[0066] Each 300ml triangular bottle contains 50ml of seed medium, sterilized at 120°C for 30 minutes, and after cooling down, connect a ring of bacterial lawn to the seed medium from the slope, and cultivate it on a temperature-controlled shaker at 36°C with a speed of 220r / min for 25 hours. It is Bacillus subtilis seed solution.
[0067] The slant medium is: peptone 1g, beef extract 0.3g, NaCl 0.5g, agar 2.0g, distilled water 100ml, pH 7.0.
[0068] The seed medium is: glucose 0.2g, NaCl 0.5g, yeast extract 0.5g, peptone 1g, distilled water 100mL, pH7.0.
[0069] (2) Yeast seed liquid
[0070] Saccharomyces cerevisiae (Saccharomyces cerevisiae...
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