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Method for tissue culture of Euonymus bungeanus

A technique for culturing Euonymus peach leaves and tissue, applied in gardening methods, botanical equipment and methods, gardening, etc., can solve problems such as shedding, difficulty in transplanting, and breaking

Inactive Publication Date: 2018-12-21
内蒙古和盛生态科技研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this kind of small root is relatively fragile, usually it will be broken or the whole root will fall off under the action of external force, and it is difficult to survive transplanting

Method used

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  • Method for tissue culture of Euonymus bungeanus
  • Method for tissue culture of Euonymus bungeanus
  • Method for tissue culture of Euonymus bungeanus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Tissue culture of Euonymus persicae by rooting in a bottle:

[0055] Step 1. Collecting explants: In Inner Mongolia, Euonymus persicae began to germinate and shoot in April. At this time, the branches of the current year can be collected as explants. The mother plant is required to be free from diseases and insect pests and grow vigorously.

[0056] Step 2, explant sterilization: before inoculation, turn on the ultraviolet light of the ultra-clean workbench for 15-20 minutes, and then ventilate for 10 minutes. Cut off the leaves on the explants, lightly brush the surface of the explants with a brush dipped in detergent, put them on the ultra-clean workbench, sterilize with 75% alcohol solution for 30 seconds, and rinse with sterile water for 30 seconds. Once again, treat with a sodium hypochlorite solution with a mass concentration of 20% for 10 minutes. After the treatment is finished, it is washed 4-5 times with sterile water to clean up the residual disinfection re...

Embodiment 2

[0069] The plant growth of embodiment 2 is normal, due to the adding of activated carbon, the growth period is obviously shortened, and do not produce callus tissue in the rooting process, but its rooting bar number is compared with embodiment 1 and has no significant difference.

Embodiment 3

[0070] What embodiment 3 used is the most suitable rooting medium, wherein added the gac of 0.3g / L. The performance is that the growth period is 27 days, the number of rooted strips is 5, the rooting rate reaches 95%, and the plant grows vigorously.

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Abstract

The invention provides a method for tissue culture of Euonymus bungeanus, comprising the following steps: (1) performing sterilization treatment on an Euonymus bungeanus explant to obtain a sterile explant; (2) inoculating the aseptic explant in a primary culture medium for primary culture to obtain adventitious buds; (3) inoculating the adventitious buds in a subculture medium for subculture to obtain subcultured seedlings; and (4) inoculating the subcultured seedlings in a rooting culture medium for rooting culture to obtain rooted seedlings. The method solves the problems that it is difficult for Euonymus bungeanus to grow roots, the breeding efficiency is low, and industrialized production cannot be realized, and the survival rate of the obtained transplanted seedlings reaches 85-90 percent.

Description

technical field [0001] The invention relates to the technical field of tree breeding, in particular to a tissue culture method for Euonymus peach leaf seedlings. Background technique [0002] Euonymus peach leaf, also known as Simianmu, Baidu, Mingkai Yehe, and North China Euonymus, belongs to the genus Euonymus of the family Euonymus, with a height of 6 meters. It is a small deciduous tree or shrub. Crown round or ovate, bark taupe, branchlets green, almost quadrangular. The leaves are opposite, elliptic-ovate or broadly ovate, with fine serrations on the margin. Cymes axillary, with 3-7 flowers, yellow-green. Capsule 4-valved, light red or yellowish; seeds with orange-red arils. The flowering period is from May to June, and the fruit ripening period is from September to October. The current propagation method is mainly based on sowing and cutting propagation. Since my country has adopted the method of tissue culture to breed poplar and other tree species, that is, the...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 牛新月赵泉胜铁英田菊刘洋
Owner 内蒙古和盛生态科技研究院有限公司
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