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Method for measuring multiple preservatives in food by adopting high performance liquid chromatography

A high-performance liquid chromatography and preservative technology, applied in the field of food safety analysis, can solve problems such as harming human health, and achieve the effect of ensuring accuracy and avoiding impact.

Inactive Publication Date: 2018-12-18
苏州汉德瑞生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Preservatives are widely used as food additives to prevent the spoilage of food caused by microorganisms and prolong the shelf life of food. However, the preservatives currently used are mainly artificially synthesized chemical reagents. Long-term excessive intake will endanger human health.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: test preservatives in liquid samples such as fruit juice, milk, soy sauce

[0022] 1. Use a pipette to remove 1-5mL of liquid samples into a 15mL centrifuge tube, add 5mL of methanol, centrifuge at a speed greater than 3000r / min for at least 1min, then add 3g of anhydrous magnesium sulfate, continue centrifuging for at least 2min, take the supernatant, After filtering through 0.4um filter paper, take the filter and carry out sample analysis. During the test, 10uL was injected into the instrument, the flow rate of the mobile phase was controlled at 1mL / min, and the pH value was adjusted to about 2. Measure at least three times, then optimize, compare the optimized results with the standard curve and use the peak area to calculate the concentration of each preservative in the sample to be tested.

Embodiment 2

[0023] Embodiment two: test preservatives in solid samples such as bread, biscuit, moon cake

[0024] Weigh 1-2g of solid sample into a 15mL centrifuge tube, add 5mL of n-hexane, centrifuge at a speed greater than 1000r / min for at least 1min, add 10mL of methanol after removing the upper layer of n-hexane, first at a speed of less than 500r / min After rotating for more than 15 minutes, centrifuge at a speed greater than 3000r / min for at least 2 minutes, then add 3g of anhydrous sodium sulfate, continue centrifuging for at least 2 minutes, take the supernatant, filter it through 0.4um filter paper, and filter it for further processing. sample analysis. During the test, 10uL was injected into the instrument, the flow rate of the mobile phase was controlled at 1mL / min, and the pH value was adjusted to about 2. Measure at least three times, then optimize, compare the optimized results with the standard curve and use the peak area to calculate the concentration of each preservative...

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Abstract

The invention discloses a method for measuring multiple preservatives in a food by adopting high performance liquid chromatography. The method comprises the following steps of pretreating a sample: dissolving the to-be-measured sample in an organic solvent according to a certain mass ratio, and taking supernate to perform filtering after centrifugation, wherein obtained filtrate is a to-be-measured solution; preparing experimental conditions: selecting a chromatographic column, performing startup preheating, and preparing a solution used as a mobile phase and a standard solution; and measuringand analyzing the preservatives: injecting a certain volume of the standard solution and the to-be-measured solution into a high performance liquid chromatograph, controlling a flow speed, obtaininga to-be-measured curve and a standard curve, and analyzing the content of the preservatives in the to-be-measured curve according to the standard curve. According to the method, by selecting a reasonable reagent as the mobile phase and a proper method, the sample is treated, and the preservatives in the food are effectively purified, so that the influence of the reagent on a detection result is avoided, and the accuracy of a final result is ensured.

Description

technical field [0001] The invention belongs to the field of food safety analysis, and in particular relates to a method for measuring various preservatives in food by using high-performance liquid chromatography. Background technique [0002] Preservatives are widely used as food additives to prevent the spoilage of food caused by microorganisms and prolong the shelf life of food. However, the preservatives currently used are mainly artificially synthesized chemical reagents. Long-term excessive intake will endanger human health. . Among them, benzoic acid, sorbic acid, dehydroacetic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, and propyl p-hydroxybenzoate are the seven most widely used preservatives. At present, in order to increase the guarantee period of food, some unscrupulous merchants add excessive additives to food in violation of regulations. Therefore, the present invention proposes a method for the determination of these seven main preservatives in fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 杨保强
Owner 苏州汉德瑞生物工程有限公司
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