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Novel application of oleanolic acid

A technology of oleanolic acid and drugs, applied in the application field of oleanolic acid, to achieve the effects of inhibiting overexpression, reducing skin damage, and reducing overexpression

Inactive Publication Date: 2018-12-18
WUYI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And because the inflammatory response involves the participation of many inflammatory mediators and the activation of different signaling pathways, the current treatment of such diseases is still a very difficult problem
Although many anti-inflammatory drugs have been available and have good curative effects, the existence of adverse reactions and side effects is the main reason that limits the wide application of these drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Effect of oleanolic acid on TPA-induced ear swelling in mice.

[0028] Experimental animals were randomly divided into normal group, TPA group, positive drug group (TPA+prednisolone, 5 μmol / 100 μL), low-dose oleanolic acid group (LPS+oleanolic acid, OA5, 5 μmol / 100 μL), oleanolic acid Acid medium dose group (LPS + oleanolic acid, OA10, 10 μmol / 100 μL) and oleanolic acid high dose group (LPS + oleanolic acid, OA20, 20 μmol / 100 μL), 3 rats in each group. Each experimental group was administered before TPA smearing. Except the normal group, all other groups began to apply TPA 15 minutes after administration. After different times, the mice were killed by cervical dislocation, and then both ears of the mice were cut off in time, and round ears were punched at the same position with a puncher with a diameter of 6 mm; weighed, the blank group was negative, and the experimental group The difference between the ear piece weight and the blank group ear piece weight w...

Embodiment 2

[0036] Example 2 Effect of oleanolic acid on TPA-induced inflammatory cells in the mouse auricle

[0037] The experimental grouping and dosing regimen are the same as in Example 1. Six hours after administration, the mice were killed by cervical dislocation, and then both ears of the mice were cut off in time. Fix in 4% paraformaldehyde for 24 hours, rinse with tap water, and dehydrate with alcohol gradients of different concentrations. After dehydration, immerse in xylene for transparency. The transparent sample is immersed in molten paraffin for 4 hours, embedded, sectioned, dewaxed and rehydrated, then HE stained and observed under an optical microscope. The result is as figure 1 shown.

[0038] Depend on figure 1 From the results, it can be seen that the tissue of the blank group (acetone treatment group) did not change significantly, which showed the appearance of normal tissue, and the thickness of the auricle and the cuticle did not change. When the inflammatory ag...

Embodiment 3

[0039] Example 3 Effect of oleanolic acid on the content of iNOS and COX-2 in TPA-induced mouse auricle tissue

[0040] The experimental grouping and dosing regimen are the same as in Example 1. The mice were sacrificed by cervical dislocation, and then both ears of the mice were cut off in time. Fix in 4% paraformaldehyde for 24 hours, rinse with tap water, and dehydrate with alcohol gradients of different concentrations. The content of iNOS and COX-2 in the mouse auricle was determined by immunohistochemical experiment, and the experimental data were expressed as Mean±SD. The SPSS19.0 software was used for data processing, and the ANOVA test was used for statistical analysis. P<0.05 indicated a statistical The results are shown in Table 2.

[0041]Immunohistochemical test: take out the complete auricle tissue, fix it in 4% paraformaldehyde, and start paraffin section after washing with PBS: the main steps include gradient ethanol dehydration (70%, 95% and 100%) → gradient ...

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Abstract

The invention relates to the field of medicines, in particular relates to novel application of oleanolic acid and especially relates to application of preparation of a medicine for inhibiting skin inflammations. An experiment shows that the oleanolic acid can be used for effectively inhibiting the release of inflammatory factors, so that the inflammation diminishing aim is realized. The result shows that the oleanolic acid can be used for preparing a skin inflammation inhibitor.

Description

technical field [0001] The invention relates to the field of medicine, in particular to an application of oleanolic acid. technical background [0002] Inflammation refers to a complex self-defense response of living tissues with vascular system to damaging stimuli (such as harmful stimuli, germs or physical damage). The generation of inflammation is closely related to the onset of many chronic progressive inflammatory diseases (such as arthritis, ulcerative colitis, sepsis, atherosclerosis, etc.), and its existence seriously threatens human health. As my country's population increases and population aging becomes more prominent, the incidence of such inflammatory diseases is also increasing year by year, and it has become one of the public health problems that cannot be ignored. And because the inflammatory response involves the participation of many inflammatory mediators and the activation of different signaling pathways, the current treatment of such diseases is still a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/56A61P17/00A61P17/06A61P25/28
CPCA61K31/56A61P17/00A61P17/06A61P25/28
Inventor 刘文锋黎妍文李冬利张琨
Owner WUYI UNIV
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