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Detection kit for cytomegalovirus antibody IgM

A detection kit and cytomegalovirus technology, applied in the field of cytomegalovirus antibody IgM detection kits, can solve the problems of increasing detection time and reducing detection efficiency, and achieve the effect of improving detection efficiency and shortening detection time

Pending Publication Date: 2018-12-14
宙斯生命科技(常州)有限公司
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Problems solved by technology

[0002] Toxoplasma gondii, rubella, cytomegalovirus, herpes simplex virus type 1, and herpes simplex virus type 2 are common viruses in today's society. Herpes simplex virus type 2 can be detected by existing detection reagents before further treatment, however when detecting toxoplasma gondii, rubella, cytomegalovirus and herpes simplex virus type 1 and herpes simplex virus type 2 in a sample At the same time, it is necessary to use four separate detection reagents to detect Toxoplasma gondii, rubella, cytomegalovirus, herpes simplex virus type 1 and herpes simplex virus type 2 respectively, which increases the detection time and reduces the detection efficiency

Method used

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Embodiment Construction

[0017] In order to understand the technical means, creative features, objectives and effects of the present invention clearly, the present invention will be further described below in conjunction with specific embodiments.

[0018] A detection kit for antibody IgM such as cytomegalovirus, the detection kit includes composite microbead suspension, fluorescent display agent, sample diluent and washing liquid,

[0019] (1) Composite microbead suspension: the suspension contains identifiable polystyrene microbeads, and the polystyrene microbeads are respectively coated with the following antigens: Toxoplasma gondii antigen, rubella antigen, cytomegalocyte antigen, herpes simplex virus- 1 antigen and herpes simplex virus-2 antigen;

[0020] (2) Fluorescence display agent: Use phycoerythrin-labeled goat anti-human IgM (specific γ chain) with an initial concentration of 1.0 ug / mL, dilute and calibrate to a working solution concentration of 0.6 ug / mL, store at 2-8°C ;

[0021] (3) S...

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Abstract

The invention discloses a detection kit for a cytomegalovirus antibody IgM, and belongs to the technical field of detection reagents. The detection kit comprises a composite microsphere suspension, afluorescent display agent, a sample diluent and a cleaning solution. The production process comprises the preparation of a microsphere preservation solution, activation and coating of microspheres, preparation of a fluorescent label and preparation of a concentrated cleaning solution; in combination with specific antigens of a calibrator 1, a calibrator 2, a calibrator 3, a reference microsphere and an NSC microsphere reference sample, the problem that four detection reagents need to be used independently to detect toxoplasma gondii, rubella, cytomegalovirus, Herpes simplex virus type 1 and Herpes simplex virus type 2 when detecting the toxoplasma gondii, rubella, cytomegalovirus, Herpes simplex virus type 1 and Herpes simplex virus type 2 in one sample is solved, and the detection time isshortened, thereby improving the detection efficiency.

Description

technical field [0001] The invention relates to a detection kit for antibody IgM such as cytomegalovirus, which belongs to the technical field of detection reagents. Herpes simplex virus type 1, herpes simplex virus type 2 IgM antibody detection kit. Background technique [0002] Toxoplasma gondii, rubella, cytomegalovirus, herpes simplex virus type 1, and herpes simplex virus type 2 are common viruses in today's society. Herpes simplex virus type 2 can be detected by existing detection reagents before further treatment, however when detecting toxoplasma gondii, rubella, cytomegalovirus and herpes simplex virus type 1 and herpes simplex virus type 2 in a sample At the same time, it is necessary to separately use four detection reagents to detect Toxoplasma gondii, rubella, cytomegalovirus, herpes simplex virus type 1 and herpes simplex virus type 2, which increases the detection time and reduces the detection efficiency. Contents of the invention [0003] The technical p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/543G01N33/58
CPCG01N33/54313G01N33/56983G01N33/56994G01N33/582G01N33/6854Y02A50/30
Inventor 宣生良
Owner 宙斯生命科技(常州)有限公司
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