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A kind of diclazuril enantiomer chiral chromatographic separation analysis method

A technology of separation analysis and chiral chromatography, which is applied in the field of chiral chromatography separation and analysis of diclazuril enantiomers to achieve good separation effect and fast analysis speed

Active Publication Date: 2021-11-26
广州研创生物技术发展有限公司
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Problems solved by technology

However, there is no report on the liquid chromatography chiral separation technology of diclazuril enantiomers on a chiral chromatographic column

Method used

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  • A kind of diclazuril enantiomer chiral chromatographic separation analysis method
  • A kind of diclazuril enantiomer chiral chromatographic separation analysis method
  • A kind of diclazuril enantiomer chiral chromatographic separation analysis method

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Embodiment 1～14

[0034] The chiral chromatographic separation and analysis method of diclazuril enantiomers in Examples 1-14

[0035] The specific steps of the chiral chromatographic separation and analysis method of diclazuril enantiomers provided in each example are as follows: dissolve diclazuril in N,N-dimethylformamide and prepare 5.0 mg·mL -1 solution, and then diluted with ethanol to 0.5 mg·mL -1 , after controlling the conditions such as mobile phase composition, mobile phase flow rate and chromatographic column temperature, the chromatographic separation of diclazuril was carried out.

[0036] In the chiral chromatographic separation and analysis methods of Examples 1 to 14, the chromatograph used is Agilent 1200 series liquid chromatograph, the detector is Agilent 1260 type ultraviolet detector and German CHIRRALYSER-MP optical detector, and the chromatographic column is Guangzhou Research Institute. Produced by Chuang Biotechnology Development Co., Ltd. SCDP, OD and AD chroma...

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Abstract

The invention discloses a chiral chromatographic separation and analysis method of diclazuril enantiomers. The separation and analysis method comprises the following steps: pretreating the chiral stationary phase, using n-hexane, trifluoroacetic acid and lower alcohols as mobile phases , carry out liquid chromatography separation and analysis; the pretreatment is to wash the chiral stationary phase with the mobile phase, the washing time is not less than 2 hours, and the washing flow rate is not more than 0.5 mL·min ‑1 ; The chiral stationary phase is bonded p-chloro-phenylcarbamoylated β-cyclodextrin, coated cellulose three (3,5-dimethylphenylcarbamate) or coated direct Amylopectin tris (3,5-dimethylphenylcarbamate); the lower alcohol is ethanol, n-propanol, isopropanol or n-butanol; the volume ratio of the n-hexane and the lower alcohol is 50~ 80:50~20. In the chiral chromatographic separation and analysis method provided by the invention, the chiral stationary phase is pretreated, and n-hexane, trifluoroacetic acid and lower alcohols are used as mobile phases to realize the chiral chromatographic separation of diclazuril enantiomers.

Description

technical field [0001] The invention belongs to the technical field of chiral chromatographic separation, and more specifically relates to a chiral chromatographic separation and analysis method of diclazuril enantiomers. Background technique [0002] With the development of stereochemistry, the study of stereoselectivity of chiral drugs has attracted much attention. Chiral isomers with the same physical and chemical properties may exhibit completely different absorption, metabolism, pharmacological effects, teratogenic, carcinogenic, mutagenic and endocrine disrupting activities in vivo. The four stereoisomers of difenoconazole had significant differences in their antibacterial activities against tomato early blight, Botrytis cinerea, leaf mold, rice sheath blight and watermelon anthracnose, namely (R,S )-difenoconazole is better than (R,R)-difenoconazole, (S,R)-difenoconazole, (S,S)-difenoconazole [EnvironmentalScience&Technology, 2013,47 (7):3386-3394]. In the broad-sp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 章伟光钟宇静范军黄湛赖烨才
Owner 广州研创生物技术发展有限公司
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