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Diclazuril enantiomer chiral chromatographic separation analysis method

A technology for separation and analysis of diclazuril, applied in the field of chiral chromatographic separation, to achieve the effect of fast analysis speed and good separation effect

Active Publication Date: 2018-12-14
广州研创生物技术发展有限公司
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Problems solved by technology

However, there is no report on the liquid chromatography chiral separation technology of diclazuril enantiomers on a chiral chromatographic column

Method used

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  • Diclazuril enantiomer chiral chromatographic separation analysis method
  • Diclazuril enantiomer chiral chromatographic separation analysis method
  • Diclazuril enantiomer chiral chromatographic separation analysis method

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Embodiment 1～14

[0034] The chiral chromatographic separation and analysis method of diclazuril enantiomers in Examples 1-14

[0035] The specific steps of the chiral chromatographic separation and analysis method of diclazuril enantiomers provided in each example are as follows: dissolve diclazuril in N,N-dimethylformamide and prepare 5.0 mg·mL -1 solution, and then diluted with ethanol to 0.5 mg·mL -1 , after controlling the conditions such as mobile phase composition, mobile phase flow rate and chromatographic column temperature, the chromatographic separation of diclazuril was carried out.

[0036] In the chiral chromatographic separation and analysis methods of Examples 1 to 14, the chromatograph used is Agilent 1200 series liquid chromatograph, the detector is Agilent 1260 type ultraviolet detector and German CHIRRALYSER-MP optical detector, and the chromatographic column is Guangzhou Research Institute. Produced by Chuang Biotechnology Development Co., Ltd. SCDP, OD and AD chroma...

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Abstract

The invention discloses a diclazuril enantiomer chiral chromatographic separation analysis method. The separation analysis method comprises the following steps of carrying out pretreatment on a chiralstationary phase, taking normal hexane, trifluoroacetic acid and lower alcohol as a mobile phase, and carrying out liquid phase chromatography separation analysis, wherein the pretreatment refers towashing of the chiral stationary phase by using the mobile phase, the washing time is not less than 2 hours, and the washing flow rate is not greater than 0.5mL.min-1; the chiral stationary phase is bonding type to parachloro-phenylcarbamoyl beta-cyclodextrin, coating type cellulose tri(3, 5-dimethyl phenylcarbamate) or coating type straight-chain starch tri(3, 5-dimethyl phenylcarbamate); the lower alcohol is ethanol, n-propyl alcohol, isopropanol or n-butyl alcohol; and the volume ratio of normal hexane to the lower alcohol is 50-80: 50-20. According to the chiral chromatographic separationanalysis method provided by the invention, the chiral stationary phase is pretreated, and the normal hexane, the trifluoroacetic acid and the lower alcohol are used as the mobile phase, so that the chiral chromatographic separation of the diclazuril enantiomer is realized.

Description

technical field [0001] The invention belongs to the technical field of chiral chromatographic separation, and more specifically relates to a chiral chromatographic separation and analysis method of diclazuril enantiomers. Background technique [0002] With the development of stereochemistry, the study of stereoselectivity of chiral drugs has attracted much attention. Chiral isomers with the same physical and chemical properties may exhibit completely different absorption, metabolism, pharmacological effects, teratogenic, carcinogenic, mutagenic and endocrine disrupting activities in vivo. The four stereoisomers of difenoconazole had significant differences in their antibacterial activities against tomato early blight, Botrytis cinerea, leaf mold, rice sheath blight and watermelon anthracnose, namely (R,S )-difenoconazole is better than (R,R)-difenoconazole, (S,R)-difenoconazole, (S,S)-difenoconazole [EnvironmentalScience&Technology, 2013,47 (7):3386-3394]. In the broad-sp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 章伟光钟宇静范军黄湛赖烨才
Owner 广州研创生物技术发展有限公司
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