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Multi-photon exited near-infrared II fluorescence scanning micro-imaging system

A fluorescence scanning and microscopic imaging technology, which is applied in the fields of fluorescence/phosphorescence, material excitation analysis, and material analysis through optical means, can solve the problems of large loss of fluorescence signals and inability to effectively detect fluorescence signals in the second near-infrared region. Achieve the effect of large biological tissue imaging depth, easy operation, and small biological tissue damage

Inactive Publication Date: 2018-12-11
ZHEJIANG UNIV
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Problems solved by technology

If it is directly used for multi-photon excitation near-infrared two-region fluorescence imaging, the loss of fluorescence signal will be great
In addition, the photomultiplier tube (PMT) of a commercial multiphoton fluorescence scanning microscope can only respond to the visible light band, so it cannot effectively detect the fluorescence signal in the second near-infrared region.
At present, there is no near-infrared two-zone fluorescence scanning microscopy system suitable for multiphoton excitation on the market

Method used

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Embodiment Construction

[0016] The present invention will be further described below in conjunction with accompanying drawing.

[0017] The present invention utilizes functionally independent hardware equipment to build a set of multi-photon excited near-infrared second region (1000-1700nm) fluorescent scanning microscopic imaging system. It is based on a scanning microscope, using a femtosecond pulsed laser with tunable wavelength as a light source (here we take a 1220nm femtosecond laser as an example to test the effectiveness of the system), using a near-infrared anti-reflection large-diameter fiber collimator and The near-infrared anti-reflection large-caliber optical fiber introduces the near-infrared second-range fluorescence of the sample into the photomultiplier tube (PMT) that responds to the near-infrared second region for detection. After the optical signal is converted into an electrical signal, it is amplified by a high-gain, large-bandwidth signal amplifier, transmitted to a high-speed ...

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Abstract

The invention discloses a multi-photon exited near-infrared II fluorescence scanning micro-imaging system. In the invention, a 1220 nm femtosecond laser in the invention is introduced into an Olympusupright scanning microscope, and focused on a sample through a near-infrared anti-reflection objective lens; a near-infrared II fluorescence signal is collected by a large-diameter fiber collimator and coupled into a near-infrared anti-reflection large-diameter optical fiber, and finally detected and converted into an electrical signal by a photomultiplier tube responded by near-infrared II; and the electrical signal is amplified by a signal amplifier and input to a high speed data acquisition card for acquisition, and processed by a computer to obtain a near-infrared II fluorescence intensityimage of the sample. The invention has the characteristics of simplicity and practicality, good stability, tunable excitation wavelength and flexible use mode.

Description

technical field [0001] The invention belongs to the field of micro-imaging of applied optics, and relates to a multi-photon-excited near-infrared two-zone fluorescent scanning micro-imaging system. Background technique [0002] The second near-infrared region generally refers to the 1000-1700nm band. The light with a wavelength in the second near-infrared region has less scattering in biological tissues. Therefore, fluorescence biological imaging based on the second near-infrared region has the advantages of large imaging depth and high spatial resolution. . In addition, the autofluorescence of biological tissue in the second near-infrared band is relatively small, so the fluorescence imaging of the second near-infrared region also has a good signal-to-noise ratio. [0003] In recent years, near-infrared two-region fluorescence bioimaging technology has developed rapidly and played an important role in biomedical research. At present, the fluorescence macroscopic in vivo i...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/01G01N21/6486
Inventor 钱骏虞文斌张鹤群
Owner ZHEJIANG UNIV
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