A kind of ph-sensitive Bacillus subtilis bacterial agent
A technology of Bacillus subtilis and spores, applied in the directions of bacteria, biocides, animal repellents, etc., can solve problems such as unfavorable crop growth, fertilizer loss, reducing fertilizer utilization efficiency, etc., to improve nutrient conditions and nutrient supply conditions, inhibit activities, the effect of improving soil salinization problems
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Embodiment 1
[0037] Embodiment 1, the preparation of pH-sensitive bacillus subtilis inoculum
[0038] (1) Bacillus subtilis surface coated with dopamine
[0039] Weigh 1.5g Tris and place it in a beaker, add about 900mL of deionized water, and adjust the pH of the Tris-HCl solution to 8-8.5 with 6M HCl; -HCl solution, and dilute the solution to 1 L; add 5 g of dopamine hydrochloride, stir at room temperature for more than 2 hours, and suction filter to obtain Bacillus subtilis coated with dopamine on the surface.
[0040] (2) Coating of pH-sensitive polymer (Pdop-g-PAA-co-HMAm)
[0041]Weigh 2.0 g of NaOH solid into 20 mL of ultrapure water, and stir magnetically at room temperature until NaOH is completely dissolved. After the solution was cooled to room temperature, 3.6 g of acrylic acid (AA) was added dropwise thereto, and magnetically stirred at room temperature for 1 h (covered with tin foil to avoid light), to obtain a 2.5 mol / L sodium acrylate solution (SA).
[0042] Put 5-10 g o...
Embodiment 2
[0043] Embodiment 2, live bacteria release experiment of pH-sensitive Bacillus subtilis bacterial agent
[0044] Get pH-sensitive Bacillus subtilis inoculum 6g prepared according to the method described in Example 1, divide it into 3 parts on average, place it in sterile water with a pH value of 5, 7, and 9 for 1 day, and then detect the different pH values. In some cases, the release amount of live bacteria of the pH-sensitive bacterial agent.
[0045] Plate detection method to detect the release of viable bacteria detection method:
[0046] 1) Prepare a solution according to the mass volume ratio of the placed sample: sterile water at a ratio of 1:10, and filter the solution through a 3-micron filter membrane to filter out the dopamine attached to the surface of the unreleased live bacteria Bacteria particles and bacteria particles coated with polymer molecules;
[0047] 2) Collect the filtrate, add glass beads, let it stand for 20 minutes, and shake it on a rotary shaker ...
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