Monkey encephalic zone virus injection method and applications thereof

Abstract

The invention relates to a monkey virus injection method and applications thereof, and more specifically relates to a monkey encephalic zone virus injection method, and applications thereof. The monkey encephalic zone virus injection method mainly comprises following steps: virus is coated so as to realize coupling of exposed surface residues on virus housings with magnetic induction proteins, motor proteins, and ATP, and obtaining of modified virus (nanometer robots), and spinal puncture technology is adopted for injection into monkey spinal fluid; and at last, under external magnetic field control, guiding of the modified virus in a target encephalic zone is carried out. Compared with the prior art, the advantages are that: no damage on monkey brain is caused, activation of immunity reaction of monkey brains is avoided, virus release is realized, and at the same time, virus expression efficiency in monkey brains is increased. Under cooperation of magnetic induction proteins and computer controlled magnetic force, the modified virus can be dispersed, expression in encephalic zones of relatively large area is realized, and virus expression efficiency is increased greatly.

Description

technical field [0001] The invention relates to the field of non-human primate model animal virus research, in particular to a monkey virus injection method and application, in particular to a monkey brain region virus injection method and application. Background technique [0002] As a non-human primate model animal, monkeys are often used in the study of human diseases, but there is still no breakthrough in the research on neural circuit diseases. The main reason is that the tools and viruses used to study neural circuits are difficult brain expression. The traditional method of virus injection in the monkey brain area is similar to that of rodents. First, the monkey is anesthetized with a mixed anesthetic; second, after routine disinfection, the monkey’s scalp is cut open, the skull is preserved, the monkey brain is fixed, and the zygomatic arch and positive The mid-sagittal suture was defined as a bony landmark, and the injection site was determined with reference to th...

AI Technical Summary

Problems solved by technology

[0003] However, using the above method, the effect of virus expression in the brain area of ​​monkeys often does not meet the experimental requirements

Among them, the primary technical difficulty is that the virus itself will cause the immune response of the monkey body to produce antibodies to neutralize the virus

Secondly, it is difficult for the virus to spread to a large area when it is only injected from one site; injecting a larger area than the brain requires a large amount of virus consumption, and the requirement for virus titer is also high

Thirdly, virus injection needs to open a window on the skull of monkeys, which will activate the immune system and produce antibodies to neutralize the virus; some studies have shown that the effect of modifying the surface antigen can be achieved by modifying the antigen gene of the virus, and finally reduce the immune response and improve the virus. Expression efficiency; however, the immune response induced by surgical trauma is often not alleviated by the above means