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Microbial organic fertilizer and preparation method thereof

A microbial organic fertilizer, plant technology, applied in the preparation of organic fertilizers, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as wasting energy, achieve good effects, do not contain hormones, and enhance the effect of disease and insect pest resistance

Active Publication Date: 2018-12-07
江西线元生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The ramie degumming waste liquid is also treated by means of microorganisms, chemical treatment, enzyme treatment, etc. to reduce the pollutant content in the wastewater, but it is discharged after disposal, which wastes energy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] A preparation method of microbial organic fertilizer, comprising the following steps:

[0069] 1) culture medium

[0070] Glucose medium: 1.5g glucose, 1g peptone, 0.5g yeast extract, K 2 HPO 4 0.2g, MgSO 4 ·7H 2 O0.05g, water 150ml, adjust pH to 9.5, cool after sterilization.

[0071] Ramie glue medium: ramie 10g, (NH 4 ) 2 SO 4 1.5g, K 2 HPO 4 0.2g, MgSO 4 ·7H 2 O0.25g, water 150ml, with Na 2 CO 3 Adjust the pH to 10.5, sterilize and cool to below 45°C for inoculation.

[0072] EM strain activation medium: brown sugar 1kg, salt 10g, urea 10g, 10kg water, after mixing, sterilize and cool to below 40°C.

[0073] 2) Treatment of strains

[0074] 2.1 Basophilic Bacillus subtilis

[0075] Suspend 1 g of Bacillus subtilis (commercially available) in 10 ml of sterile water, mix evenly, take 1 ml and insert it into glucose medium (150 ml), and culture at 28° C. for 36 hours to obtain activated Bacillus subtilis.

[0076] Insert 2ml of activated Bacillus su...

Embodiment 2

[0084] A preparation method of microbial organic fertilizer, comprising the following steps:

[0085] 1) culture medium

[0086] Glucose medium: 1.2g glucose, 0.75g peptone, 0.3g yeast extract, K 2 HPO 4 0.15g, MgSO 4 ·7H 2 O0.03g, water 150ml, adjust pH to 9, cool after sterilization.

[0087] Ramie glue medium: ramie 7.5g, (NH 4 ) 2 SO 4 1.2g, K 2 HPO 4 0.15g, MgSO 4 ·7H 2 O0.15g, water 150ml, with Na 2 CO 3 Adjust the pH to 10, sterilize and cool to below 45°C for inoculation.

[0088]EM strain activation medium: brown sugar 0.8kg, salt 8g, urea 8g, 10kg water, after mixing, sterilize and cool to below 40°C.

[0089] 2) Treatment of strains

[0090] 2.1 Basophilic Bacillus subtilis

[0091] Suspend 0.5 g of Bacillus subtilis in 10 ml of sterile water, mix evenly, take 0.5 ml and insert it into glucose medium (150 ml), and culture at 28±2° C. for 48 hours to obtain activated Bacillus subtilis.

[0092] Insert 4.5ml of activated Bacillus subtilis into ramie...

Embodiment 3

[0100] A preparation method of microbial organic fertilizer, comprising the following steps:

[0101] 1) culture medium

[0102] Glucose medium: 2.25g glucose, 1.2g peptone, 0.75g yeast extract, K 2 HPO 4 0.3g, MgSO 4 ·7H 2 O0.075g, water 150ml, adjust pH to 10, cool after sterilization.

[0103] Ramie glue medium: ramie 12g, (NH 4 ) 2 SO 4 2.25g, K 2 HPO 4 0.3g, MgSO 4 ·7H 2 O0.3g, water 150ml, with Na 2 CO 3 Adjust the pH to 11, sterilize and cool to below 45°C for inoculation.

[0104] EM strain activation medium: brown sugar 1.5kg, salt 15g, urea 15g, water 10kg, mix and sterilize and cool to below 40°C.

[0105] 2) Treatment of strains

[0106] 2.1 Basophilic Bacillus subtilis

[0107] Suspend 2 g of Bacillus subtilis in 10 ml of sterile water, mix evenly, take 1.2 ml and insert it into glucose medium (150 ml), and culture at 28±2° C. for 24 hours to obtain activated Bacillus subtilis.

[0108] Insert 1.5ml of activated Bacillus subtilis into ramie glue...

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Abstract

The invention relates to the field of bacterial manure, in particular to a microbial organic fertilizer and a preparation method thereof. The preparation method comprises the following steps: adding bacillus subtilis into flax plant degumming waste liquor for aerobic fermentation; and stopping oxygen supply and inoculating an EM culture for anaerobic fermentation to obtain the microbial organic fertilizer. By taking the flax plant degumming waste liquor as a raw material and adding bacillus subtilis for aerobic fermentation, the bacillus subtilis can form an advantageous population quickly soas to inhibit growth of pathogenic bacteria or other infectious microbes; in the thallus growing process, active substances such as subtilin, polymyxin, nystatin and gramicidin are generated to play an obvious inhibiting role to pathogenic bacteria or endogenously infected conditioned pathogens. By adding the ME culture for anaerobic fermentation, the anaerobic EM bacteria are fermented to generate organic acids, so that the pH is reduced to form a slight acidic environment. In addition, the EM bacteria are compound cultures. Multiple probiotics are fermented, and the organic fertilizer applied to soil can form a symbiotic and stable bacterial colony environment, so that the effect is more lasting.

Description

technical field [0001] The invention relates to the field of bacterial fertilizer, in particular to a microbial organic fertilizer and a preparation method thereof. Background technique [0002] Existing microorganisms are often used in the preparation of fertilizers or as wastewater treatment. [0003] Common microbial organic fertilizers are made of straw and / or animal manure as raw materials, which are fermented by adding bacteria. For example, the application number 201410262147.8 discloses a microbial organic fertilizer and its preparation method, using Bacillus subtilis to ferment agricultural organic solid waste It is used to produce microbial organic fertilizers to prevent and eliminate obstacles to continuous cropping of Artemisia annua. The number of functional microorganisms in the microbial organic fertilizer is ≥2×10 8 cfu·g -1 , the water content is less than 30%, and the organic matter content is greater than 30%. The microbial organic fertilizer can accel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/04C05G3/02C12N1/20C12R1/125C05G3/60C05G3/80
CPCC05B7/00C05G3/00C12N1/20C05G3/60C05G3/80C05C9/00C05F7/00C05F11/08C05F17/00Y02W30/40
Inventor 褚特野黄成锋赵志慧邱新海张凯锋黄建新邱枚英
Owner 江西线元生物科技有限公司
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