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Liquid fertilizer for promoting differentiation of Cuiguan pear flower buds and preparation method of liquid fertilizer

A technology of liquid fertilizer and bud differentiation, applied in the field of liquid fertilizer, can solve the problems of Cuiguan pear root growth and other problems, achieve the effects of improving root growth, promoting flower bud differentiation, and benefiting quality

Inactive Publication Date: 2018-12-07
郴州市通源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the problem of the root growth of the existing Cuiguan pear, and to provide a liquid fertilizer for promoting the flower bud differentiation of Cuiguan pear and its preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The isolation and identification of embodiment 1 Streptomyces bacterial strain (Streptomyces)

[0044] 1. Experimental Materials and Conditions

[0045] (1) Soil samples collected in a certain place in Chenzhou City, Hunan Province.

[0046] (2) The components per liter of the liquid medium are as follows: 3.0 g of beef extract, 10.0 g of peptone, 5.0 g of sodium chloride, dilute to 1 liter of water, and the pH is 7.4-7.6.

[0047] The components per liter of solid medium are as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, agar 15-25g, water to 1 liter, pH 7.4-7.6.

[0048] The culture medium was divided into aliquots and sterilized in an autoclave (121°C, 30min).

[0049](3) Sterile operating conditions: All utensils and utensils must be sterilized in an autoclave (121°C, 30min), and operations such as inoculation are carried out in an ultra-clean workbench.

[0050] (4) Conditions for culturing strains: place them in a light incubator (14L: 10D)...

Embodiment 2

[0065] Embodiment 2 Preparation and application of a liquid fertilizer that promotes Cuiguan pear flower bud differentiation

[0066] A kind of preparation method of the liquid fertilizer that promotes Cuiguan pear flower bud differentiation comprises the following steps:

[0067] S1. Preparation of culture medium: soak 1000g of soybeans for 10 hours, put them into a refiner and grind them into soybean milk, filter out the bean dregs, put the filtrate in a clean fermenter, add white sugar to the filtrate, add water to The concentration of sugar in the medium is 100g / L, and the pH is 7.4-7.6;

[0068] S2. inoculation: get the liquid spawn of Streptomyces bacterial strain " TY-001# ", inoculate in the culture medium, stir evenly; There are 200,000,000 viable bacteria per milliliter in the liquid spawn; The ratio of liquid spawn and culture medium 165g: 250L

[0069] S3. Fermentation: Turn on the oxygen pump, continuously feed oxygen into the fermenter during the fermentation p...

Embodiment 3

[0075] Embodiment 3 Preparation and application of a liquid fertilizer that promotes Cuiguan pear flower bud differentiation

[0076] A kind of preparation method of the liquid fertilizer that promotes Cuiguan pear flower bud differentiation comprises the following steps:

[0077] S1. Preparation of medium: take 1000g of soybean meal for pre-fermentation, put it into a refiner for beating, filter, put the filtrate in a clean fermentation barrel, then add white sugar to the filtrate, add water to the medium The concentration of medium sugar is 100g / L, and the pH is 7.4-7.6;

[0078] The steps of pre-fermentation are:

[0079] S11. Add water to the soybean meal, and stir well and evenly until the soybean meal is squeezed tightly by hand to reach the state of dripping water;

[0080] S12. Cover the surface of the soybean meal with a film, leaving no gaps, and start the pre-fermentation process by timing;

[0081] S13. The pre-fermentation time is 4 days; 2 days after the start...

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Abstract

The invention discloses a liquid fertilizer for promoting the differentiation of Cuiguan pear flower buds and a preparation method of the liquid fertilizer. The liquid fertilizer comprises 1000 partsof an active liquid fertilizer substrate preparation, 10-20 parts of nitrogen, 10-15 parts of potassium, 3-6 parts of phosphorus, 6-10 parts of boron and 8-14 parts of zinc. The liquid fertilizer contains 10-15 parts of proline, 16-20 parts of phenylalanine, 6-9 parts of glutamic acid, 15-18 parts of serine, 4-6 parts of alanine, 10-15 parts of leucine and 10-15 parts of aspartic acid. The contentof amino nitrogen is 0.5-1.4 mg / mL, and the content of organic matter is 60-70 g / L. After the liquid fertilizer is applied, the length and the thickness of new shoots of the Cuiguan pear are obviously smaller than the length and the thickness of a control group, the length is reduced by 23.6%, and the thickness is reduced by 15.5%. According to the liquid fertilizer, the flower bud rate of the Cuiguan pear is increased by 10.5%, the number of flowers is increased by 15.0%, and an unexpected technical effect is achieved.

Description

technical field [0001] The invention belongs to the technical field of liquid fertilizers, and more specifically relates to a liquid fertilizer for promoting flower bud differentiation of Cuiguan pear and a preparation method thereof. Background technique [0002] Cuiguan pear is a honey pear, also known as "June Snow", with thin skin, crisp and juicy meat, rich and tender taste, rich and sweet juice. Cuiguan pear belongs to the sand pear family, the fruit is nearly round, the fruit shape index is 0.96, the soluble solid content is 12% to 14%, yellow-green, snow-white flesh, and tender. The weight of a single fruit is 200 grams, the maximum is about 500 grams, and the edible rate of the fruit is 96%. It will be launched in the first and middle days of July. It has a honey fragrance and a unique taste, and has the reputation of being the ancestor of all kinds of fruits. Generally, it germinates in mid-March, blooms in late March, and matures in late July. [0003] Streptom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05F17/00C12P1/04C12R1/465
CPCC05C11/00C05F17/00C05G3/00C12P1/04C05F17/50C05G5/23C12N1/205C12R2001/465C05F5/002C05F11/00C05F11/08Y02W30/40
Inventor 盛建平张利军李国恒欧琴华张燕梅
Owner 郴州市通源生物科技有限公司
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