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A Standard Antigen Diluent for Superparamagnetic Immunochromatography Chip

A technology of immunochromatography and standard antigen, which is applied in the direction of analytical materials, instruments, measuring devices, etc., can solve the problems of reduced stability, achieve the effects of promoting specific binding, increasing sensitivity, and easy preparation

Active Publication Date: 2021-06-08
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In published patents (such as patents CN102628863A, CN103487313A), the blood samples or antigen-antibody diluents are mostly aimed at the ELISA (enzyme-linked immunosorbent assay) detection method, and metal ions or metal ion chelating agents (such as Ca 2+ , Mg 2+ , sodium citrate, EDTA, etc.) have little effect on the color background, but they can cause aggregation between superparamagnetic immunomagnetic beads and reduce the stability
After searching, there are currently no relevant literature or patents on the standard antigen diluent formula for superparamagnetic immunochromatography chips

Method used

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  • A Standard Antigen Diluent for Superparamagnetic Immunochromatography Chip
  • A Standard Antigen Diluent for Superparamagnetic Immunochromatography Chip
  • A Standard Antigen Diluent for Superparamagnetic Immunochromatography Chip

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Experimental program
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Embodiment

[0059] Step 1: Preparation of carboxyl-modified superparamagnetic nanomagnetic beads

[0060] Take 35gFeCl 3 ·6H 2 O and 13gFeCl 2 4H 2 O was placed in a 1L beaker, 600ml of water was added to it, nitrogen was ventilated continuously, and then stirred at 500rpm to dissolve it. Quickly pour 50ml of ammonia water into the beaker, and react in a 70°C water bath for 1 hour. Then slowly dropwise add 45g oleic acid therein, continue water-bath reaction 2 hours. After the reaction, the ammonia water was evaporated to obtain a black oily precipitate, which was washed with acetone and ethanol three times under an external magnetic field, and dried under nitrogen protection to obtain magnetic nanoparticle powder. 0.9 g of the obtained magnetic nanoparticle powder was dissolved in 2.5 ml of a mixture of styrene and n-octane and ultrasonically treated to form a stable oil phase. Then 0.25 g of sodium bicarbonate and 0.5 g of sodium lauryl sulfate were dissolved in 100 ml of water to...

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Abstract

The invention discloses a standard antigen dilution solution for a superparamagnetic immunochromatographic chip, which contains: a phosphate buffer solution with a concentration of 10mmol / L and a tris buffer solution with a concentration of 25-50mmol / L One or two of them, bovine serum albumin at a concentration of 0.1% to 2%, glycine at a concentration of 0.1 to 0.2%, and sucrose at a concentration of 0.5 to 2%, and the pH of the standard antigen dilution solution prepared with the above ingredients is 7.4 ~8.0. This standard antigen diluent is designed for superparamagnetic immune chromatography chips, which can maintain the stability of superparamagnetic nano-magnetic beads, achieve the purpose of increasing the sensitivity and specificity of superparamagnetic immune chromatography chips, and are suitable for superparamagnetic immunochromatography chips. Chromatographic technology application and promotion.

Description

technical field [0001] The invention relates to the technical field of immunochromatography detection, in particular to a standard antigen diluent for a superparamagnetic immunochromatography chip. Background technique [0002] Immunochromatography chip technology is widely used in biological detection due to its advantages of rapidity, simplicity, and low price. Common ones include colloidal gold chips and fluorescent immunochromatography chips. These chips obtain quantitative data by detecting the optical signal on the surface of the detection line. However, the superparamagnetic immunochromatographic chip that has appeared in recent years can detect magnetic signals on the surface and inside of the entire detection line, and its sensitivity can be increased by more than 10 times. In addition, superparamagnetic nano-magnetic beads have high biocompatibility, magnetic enrichment and other functions, so they have attracted more and more attention from scientific researchers...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531G01N33/543
CPCG01N33/531G01N33/54333
Inventor 王侃洪立新颜文强杨金川何井华
Owner SHANGHAI JIAOTONG UNIV
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