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Novel blue-light electrophoresis nucleic acid dye, preparation method and use thereof

A nucleic acid dye and electrophoresis technology, applied in the field of fluorescent dyes, can solve the problem of small overall molecules, achieve high specific binding ability, easy penetration, and improve the effect of high toxicity

Active Publication Date: 2018-12-04
珠海黑马医学仪器有限公司 +1
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  • Claims
  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to address the problems of prior art defects, to provide a novel blue photophoretic nucleic acid dye and its preparation method and application, this novel blue photophoretic nucleic acid dye has a high specific binding ability to nucleic acid, and the dye has a high specific binding ability to nucleic acid It has high sensitivity and is not easy to penetrate into living cells, thereby solving the safety problem of the dye, and the overall molecule is smaller, so it is easier to penetrate into polyacrylamide gel, thereby improving the staining effect of nucleic acid in polyacrylamide gel

Method used

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  • Novel blue-light electrophoresis nucleic acid dye, preparation method and use thereof
  • Novel blue-light electrophoresis nucleic acid dye, preparation method and use thereof
  • Novel blue-light electrophoresis nucleic acid dye, preparation method and use thereof

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Embodiment Construction

[0057] In order to enable those skilled in the art to better understand the technical solution of the present invention, its specific implementation will be described in detail below in conjunction with the accompanying drawings:

[0058] A novel blue photophoretic nucleic acid dye, its structure is as shown in formula (I):

[0059]

[0060] In formula (I): R is a C2-C4 alkyl group.

[0061] Preferably, R is a n-propyl group, and the novel blue light electrophoretic nucleic acid dye structure is as shown in formula (II):

[0062]

[0063] The preparation method of the novel blue electrophoresis nucleic acid dye of formula (II) is divided into following four-step reactions altogether:

[0064] The reaction equation of step 1) is:

[0065]

[0066] in: is 4-methylquinoline; Iodopropane; is intermediate I;

[0067] The reaction operation of step 1) is as follows:

[0068] In a 100ml single-necked flask, add 10g (69.8mmol) 4-methylquinoline and 100ml acetonitril...

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Abstract

The invention discloses a novel blue-light electrophoresis nucleic acid dye, a structural formula is shown in the specification, wherein R is C2-C4 alkyl groups. The novel blue-light electrophoresis nucleic acid dye has higher sensitivity and stability by comparing with common nucleic acid dyes, effectively improves the highly toxic characteristics of the traditional nucleic acid dyes, and can beused for agarose or polyacrylamide gel dyeing as the non-toxic nucleic acid dye.

Description

technical field [0001] The invention relates to the field of fluorescent dyes, in particular to a novel blue light electrophoresis nucleic acid dye and its preparation method and application. Background technique [0002] Fluorescent dyes are useful for detection and analysis of biological samples. Due to their high sensitivity, fluorescent dyes can be used to detect very small quantities of samples. Fluorescent dyes can also be used to image living organelles or tissue samples, which are more sensitive, less toxic, and easier to handle than radioactive probes. [0003] Nucleic acids (including DNA and RNA) and biological samples containing nucleic acids can be detected with fluorescent dyes. Nucleic acid polymers, such as DNA and RNA, are involved in the transmission of genetic information from one generation to the next, as well as in the general functions of living organisms. Nucleic acids are therefore very interesting research targets. Fluorescent nucleic acids that...

Claims

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Application Information

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IPC IPC(8): C09B57/00C07D417/06C09K11/06G01N1/30G01N27/447
CPCC07D417/06C09B57/00C09K11/06C09K2211/1029C09K2211/1037G01N1/30G01N27/447
Inventor 吴学如张辉邱沛韩亮
Owner 珠海黑马医学仪器有限公司
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