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A preparation method and application of wild grass mushroom mycelium with efficient dye removal function

A technology of mycelium and thatch mushroom, applied in the direction of biochemical equipment and methods, microorganism-based methods, chemical instruments and methods, etc., to achieve the effects of high-efficiency dye removal ability, high dye decolorization rate, and high-efficiency removal ability

Active Publication Date: 2021-06-15
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the decolorization of dyes by fermenting and cultivating wild mycelium of wild edible fungus. On the one hand, this research can expand the functional application range of wild edible fungus mycelium, especially for the biological treatment of dye wastewater; In addition, it can effectively protect the germplasm resources of wild edible fungi that cannot be artificially domesticated and cultivated at present.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. The solid and liquid fermentation culture of the mycelium of the wild grass mushroom: adopt 65% (v / v) ethanol solution to carry out surface disinfection and sterilization on the fruit body of the wild grass mushroom picked freshly, and cut the surface of the fruit body along the surface of the fruit body with a sterilized blade. Remove the epidermis, cut off the bacterial flesh with a length and width of about 1cm, inoculate it on a solid PDA medium, place it in a constant temperature incubator at 25°C, and culture it continuously for 5 days to obtain activated mycelium colonies; use sterilized Divide the mycelium colonies activated three times into uniform flakes with a hole puncher with a diameter of 10 mm, inoculate them into liquid PDA medium in a sterile environment, place them in a constant temperature shaker at 25°C, and vibrate at 85 rpm / min Cultivate and obtain the liquid fermented product of the mycelium of the straw mushroom in the exponential growth phase,...

Embodiment 2

[0038] 1. Solid and liquid fermented culture of wild grass mushroom mycelium: use 2-3% (v / v) hydrogen peroxide solution to disinfect and sterilize the surface of freshly picked wild grass mushroom fruiting bodies, and use a sterilized blade to edge the Peel off the epidermis on the solid surface, cut off the bacterial flesh with a length and width of about 2cm, inoculate it on a solid PDA medium, place it in a constant temperature incubator at 28°C, and culture it continuously for 7 days to obtain activated mycelium colonies; Use a sterilized hole puncher with a diameter of 10mm to divide the activated mycelium colonies into uniform flakes, inoculate them into liquid PDA medium in a sterile environment, place them in a constant temperature shaker at 28°C, and set the temperature at 100rpm / Shake culture under 1 min to obtain the liquid fermented product of the mycelium of the grass mushroom in the exponential growth phase, take an appropriate amount of the liquid fermented prod...

Embodiment 3

[0047] 1. Solid and liquid fermented culture of the mycelium of wild chrysanthemum straw mushrooms: use 30-250 mg / L chlorine dioxide solution to sterilize the surface of freshly picked fruiting bodies of wild chrysanthemum straw mushrooms, and cut off the surface of the fruiting bodies with a sterilized blade For the epidermis, cut off the bacterial flesh with a length and width of about 2 cm, inoculate it on a solid LB medium, place it in a constant temperature incubator at 31°C, and culture it continuously for 9 days to obtain activated mycelium colonies; use sterilized Divide the mycelial colonies activated three times into uniform flakes with a hole puncher with a diameter of 10 mm, inoculate them into liquid LB medium in a sterile environment, place them in a constant temperature shaker at 31°C, and shake them at 115 rpm / min Obtain the liquid fermentation product of the mycelium of the grass mushroom in the exponential growth phase, take an appropriate amount of the liquid...

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Abstract

The invention discloses a preparation method and application of wild straw mushroom mycelium with the function of efficiently removing dyestuffs. The wild straw mushroom mycelium which cannot be artificially domesticated and cultivated at present is subjected to solid and liquid fermentation and cultivation. The culture conditions were optimized and controlled, and the mycelia obtained from the liquid fermentation culture were used in the study of dye removal, which showed that it had efficient dye removal ability, and the dye decolorization rate was close to 100% after two days. The straw mushroom mycelium of the invention has wide application research value and potential development value for dye removal in industrial production wastewater such as textile wastewater and papermaking wastewater.

Description

technical field [0001] The invention relates to the field of dye decolorization of wastewater, in particular to a preparation method and application of wild grass mushroom mycelium with the function of efficiently removing dye. Background technique [0002] Dyes are widely used active substances in modern printing and dyeing, textile and paper industries, and are also widely used in modern chemical and daily necessities production. However, while dyes create huge economic value, they also bring a certain degree of environmental load, namely pollution. problems, especially the pollution of water resources. Every year in our country, a large amount of dyes or dye intermediates are directly or indirectly discharged into water bodies, and most of the dyes are chemically stable and have carcinogenic, teratogenic and mutagenic properties, causing serious harm to human health and the safety of the biological chain. , so how to deal with the huge volume of industrial dye wastewater...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C02F3/34C12R1/645C02F101/30
CPCC02F3/347C02F2101/308C12N1/14
Inventor 吴庆喜程小度金忆文赵锦陈彦
Owner ANHUI UNIVERSITY
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