Cell culture equipment

Abstract

The invention discloses cell culture equipment, and belongs to the technical field of biomedical cell culture. The cell culture equipment mainly comprises a sample injection chamber, a culture chamberand a motor chamber, and specifically comprises a culture dish conveying device, a culture dish-cover separation and closing device, a liquid changing device, an air filtering system, a disinfectingheating device, an automatic control system and a culture dish. According to the present invention, with the cell culture equipment, the isolation between the culture chamber and the outside world isachieved, the culture condition of the cells in the culture dish can be real-timely observed or shot and recorded, the automatic replacement of the culture liquid is achieved, and the gas in the culture dish and the gas in the culture chamber can be freely and smoothly exchanged while the water in the culture dish cannot be removed from the culture dish by evaporation. In the prior art, the pollution in the incubator is easily caused during the taking and placing of the culture dish, the manual adding or replacing of the culture liquid is cumbersome in the operation and easily causes the liquid changing error or the culture liquid pollution, and the growth condition of the cells in the culture dish is inconveniently observed. With the cell culture equipment of the present invention, the problems in the prior art can be solved.

Description

technical field [0001] The invention relates to the technical field of biomedical cell culture, in particular to a cell culture device. Background technique [0002] The technique of simulating the physiological environment in the body in vitro, cultivating cells taken out of the body, and making them survive and grow is called cell culture. In 1885, Roux W attempted to culture tissue in vitro, which is considered to be the germination of tissue cell culture technology. Harrison in 1907 and Carrel in 1912 began to use tissue culture as a method to study the culture of isolated animal cells, marking the birth of cell culture technology. Since then, with the continuous improvement of antibiotics, culture medium, culture equipment and process methods, the research and application of cell culture has gradually increased and deepened, and it has gradually become a research hotspot for researchers in the field of biology and medicine. and techniques widely used in applications. ...

AI Technical Summary

Problems solved by technology

This kind of incubator can meet the needs of cell culture, but there are some disadvantages. The first disadvantage is that the external hatch and inner glass door of the incubator need to be opened when taking and placing the culture dish. At this time, the inside of the incubator is directly exposed to the external environment, and the outside Direct communication between the ambient air and the air in the incubator is likely to cause pollution in the incubator, and the hands (wearing gloves) that take and place the culture dish directly into the incubator are likely to bring in pollutants; the second disadvantage is that the addition or replacement of the culture medium requires Taking out the culture dish from the incubator by manual means also has the hidden danger of pollution in the incubator, and also makes the experimental operation cumbersome, which is easy to cause errors in changing the liquid or pollute the culture medium; the third disadvantage is the common cell culture in the market. Both have the disadvantage of being inconvenient to observe the growth of cells in the culture dish. The culture dish is placed in the incubator, and the door of the incubator is closed, so it is not convenient to observe the cells in the culture dish at any time and in real time.

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