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Method for efficiently extracting D-pantoic acid from enzymatic hydrolysate

A technology for pantoic acid and enzymatic hydrolyzate, which is applied in the field of efficient extraction of D-pantoic acid, can solve the waste of D-pantoic acid lactone raw materials, the trouble of refining D-pantoic acid, the low extraction rate of one-time extraction, etc. problems, to simplify the extraction process, eliminate interference, and improve the extraction rate.

Active Publication Date: 2018-11-30
成都本则生科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But, under normal circumstances, ethyl acetate has only about 70% to the primary extraction rate of D-pantolactone, therefore, often makes a small amount of D-pantolactone still remain in the aqueous phase
Of course, this has no effect on the process with D-pantoate lactone as the end product, but for the process with D-pantoate as the target end product, there is an extraction rate, especially once The problem of slightly low extraction rate requires additional refining treatment in the follow-up, and causes waste of raw materials for D-pantolactone
Although the extraction rate of ethyl acetate to D-pantoate lactone has been further improved by adding sodium salt in the prior art, but, because the added sodium salt can be present in the aqueous phase, the subsequent D-pantoate The refinement of
Furthermore, in the process of extracting with ethyl acetate as the extraction solvent, it must first go through the treatment process of heating and inactivating enzymes to eliminate the negative impact of D-pantolactone hydrolase on the hydrolysis of ethyl acetate, which also makes the extraction more complicated process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In this example, the enzymatic hydrolyzate for extracting the D-pantoic acid was obtained by fermenting enzyme production and enzymatic hydrolysis transformation according to the prior art method, that is, the enzyme-producing fermentation was carried out with wild-type Fusarium sp. Crude enzyme solution for ester hydrolases. The culture medium and culture conditions can refer to the enzyme production method and enzymolysis transformation method recorded in Tang Yixin of Jiangnan University "Separation and Preparation of D-pantolactone by Microbial Enzyme".

[0022] The formula of the fermentation medium is: 1% glycerin, 1% yeast extract, 0.8% peptone, 0.4% corn steep liquor, pH 8.0, and the crude enzyme liquid is collected after fermentation for enzyme production.

[0023] The DL-pantolactone prepared by the chemical method was formulated into an aqueous solution with a concentration of 200g / L, and 30mmol / L of Ca 2+ , under the conditions of 30°C and pH 7.0, carry out...

Embodiment 2

[0029] The method for efficiently extracting D-pantoic acid from the enzymatic hydrolysis solution described in this example is the same as that in Example 1.

[0030] The method for efficiently extracting D-pantoic acid from the enzymolysis solution described in this embodiment comprises the following steps:

[0031] (1) Take the D-pantoate-containing enzymatic hydrolysis solution prepared above, add 2% diatomaceous earth and mix for pretreatment for 10 minutes, then remove the bacteria and diatomite by centrifugation, collect the supernatant, and set aside;

[0032] (2) According to the volume ratio of 2:1, add ethyl acetate (volume ratio 5:100) containing proline methyl ester hexafluorophosphate to the obtained supernatant and mix for extraction, mix well and let stand to separate , and then collect the aqueous phase, which is the aqueous solution containing D-pantoic acid. After testing, in the obtained aqueous solution containing D-pantoate, the content of D-pantoolacton...

Embodiment 3

[0034] The method for efficiently extracting D-pantoic acid from the enzymatic hydrolysis solution described in this example is the same as that in Example 1.

[0035] The method for efficiently extracting D-pantoic acid from the enzymolysis solution described in this embodiment comprises the following steps:

[0036] (1) Take the D-pantoate-containing enzymatic hydrolysis solution prepared above, add 2% diatomaceous earth and mix for pretreatment for 10 minutes, then remove the bacteria and diatomite by centrifugation, collect the supernatant, and set aside;

[0037] (2) According to the volume ratio of 1.5:1, add ethyl acetate (volume ratio 4:100) containing proline methyl ester hexafluorophosphate to the obtained supernatant and mix for extraction, mix well and let stand to separate , and then collect the aqueous phase, which is the aqueous solution containing D-pantoic acid. After testing, in the obtained aqueous solution containing D-pantoate, the content of D-pantoolact...

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Abstract

The invention belongs to the technical field of enzyme engineering, and specifically relates to a method for efficiently extracting D-pantoic acid from an enzymatic hydrolysate. According to the method for efficiently extracting D-pantoic acid from the enzymatic hydrolysate, on the basis of the prior extraction method of D-pantoic acid, the extraction of D-pantoic acid from the enzymatic hydrolysate by using existing ethyl acetate as an extracting agent is more thorough through further optimization of the extracting agent. For the technical process of taking D-pantoic acid as a target extract,the interference of other substances in the water phase after the extraction is effectively eliminated, so that the subsequent refining treatment process is simpler.

Description

technical field [0001] The invention belongs to the technical field of enzyme engineering, and in particular relates to a method for efficiently extracting D-pantoic acid from enzymatic hydrolysis solution. Background technique [0002] Pantothenic acid, also known as pantothenic acid and vitamin B5, widely exists in the biological world and is the main raw material for the synthesis of coenzyme A in animals. Its main function is to participate in the metabolism of sugar, fat and protein. A deficiency in pantothenic acid in the body can cause muscle aches or cramps, adrenal insufficiency and hypofunction, poor concentration, numb hands and feet, constipation, lack of energy, exhaustion after light exercise, anxiety, nervousness, and teeth grinding. Pantothenic acid is widely used in the fields of medicine, feed and food because of its unique biochemical functions. D-pantothenic acid lactone (D-PL) is the most important chiral intermediate for the synthesis of D-pantothenic ...

Claims

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Application Information

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IPC IPC(8): C07C51/42C07C51/48C07C59/105
CPCC07C51/42C07C51/48C07C59/10C07C59/105
Inventor 吴红光
Owner 成都本则生科技有限公司
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