Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Extracting purification method for rhizoma anemarrhenae polysaccharide

A purification method and technology of Anemarrhena, applied in biochemical equipment and methods, blood/immune system cells, animal cells, etc., can solve the problems of lack of methods for determining chemical indicators and monosaccharide composition, lack of purification steps, etc. Activation effect, effect with high activation ability

Inactive Publication Date: 2018-11-27
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above two patent applications are only for the extraction and research of Anemarrhena polysaccharides, lack of purification steps, only crude Anemarrhena polysaccharides can be obtained, and there is also a lack of methods for determining the corresponding chemical indicators and monosaccharide composition

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extracting purification method for rhizoma anemarrhenae polysaccharide
  • Extracting purification method for rhizoma anemarrhenae polysaccharide
  • Extracting purification method for rhizoma anemarrhenae polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The extraction and purification method of embodiment 1 Anemarrhena polysaccharide

[0037] a. Pretreatment: remove impurities from Anemarrhena, dry at 60°C, and grind to 40 mesh powder;

[0038] b. Degreasing:

[0039] (1) Ethanol degreasing:

[0040] Immerse the Anemarrhena powder processed in step a into ethanol whose weight is 10 times and the mass concentration is 85%, heat and reflux at 70°C and stir for 2h, continue stirring at room temperature for 12h after cooling, and remove the liquid; add 10% of its weight twice times, the mass concentration is 85% ethanol, stirred at room temperature for 5 hours, removed the liquid, and obtained Anemarrhena powder after ethanol degreasing;

[0041] (2) Acetone degreasing:

[0042] Adding the degreased ethanol Anemarrhena powder into an acetone solution with 5 times its weight and a mass concentration of 99%, stirring for 30 minutes, removing the liquid, and drying the powder naturally at room temperature to obtain degreas...

Embodiment 2

[0062] Example 2 Effect Test of Refined Components of Anemarrhena Polysaccharide

[0063] The crude Anemarrhena polysaccharide obtained in Example 1, refined component 1, and refined component 2 are processed and analyzed as follows:

[0064] 1) Determination of the proliferation of activated macrophages

[0065] Cell proliferation using WST-1 (2-(4-iodobenzene)-3-(4-nitrobenzene)-5-(2,4-disulfobenzene)-2H-tetrazolium sodium salt) cell proliferation and Cytotoxicity detection kit (EZ-cytox, Daeillab service CO., LTD, Korea), the specific method is as follows: add 100 μL RAW264.7 cells (1×106 cells / mL) (ATCC) into a 96-well plate, and incubate at 37° C. Pre-cultivate for 24 hours in a 5% CO2 incubator (Excella ECO-170, New Brunswick Scientific, Scotland), discard the supernatant, add 200 μL of sample solutions of different concentrations to the cells, and use the culture solution as a blank group. After culturing for 24 h, discard the supernatant, add 110 μL of 10% WST-1 solu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an extracting purification method for rhizoma anemarrhenae polysaccharide. The method comprises the steps that rhizoma anemarrhenae powder is subjected to degreasing, water extraction and alcohol precipitation extraction through an organic solvent to obtain crude rhizoma anemarrhenae polysaccharide; low-molecular-weight impurities are removed through dialysis of a dialysis membrane, a rhizoma anemarrhenae polysaccharide analysis solution is obtained through adsorption and separation of a DEAE-agarose gel column, and dialysis of the dialysis membrane is adopted for obtaining refined components of the rhizoma anemarrhenae polysaccharide. The invention provides the extracting method for the refined components of the rhizoma anemarrhenae polysaccharide for the first time, compared with a traditional method, and the refined components of the rhizoma anemarrhenae polysaccharide obtained through the method have the higher excitation capability than that of mouse macrophage RAW264.7, and the higher excitation effect for promoting expression of iNOS and related cell factors mRNA.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting and purifying anemarrhena polysaccharides. Background technique [0002] Polysaccharide is a kind of natural macromolecular compound, which is composed of aldose or ketose, a polymer formed by connecting more than 10 monosaccharides through glycosidic bonds, and is one of the four basic substances that constitute life. play an important role in the activity. People's early research on sugars only paid attention to its importance as a component of cells and energy substances. Later, it was found that some polysaccharides can be used as specific recognition signals on the cell surface to transmit information, and participate in the development of cells in life sciences. Various activities have a variety of biological functions, such as polysaccharides have anti-virus, anti-aging, anti-inflammatory, anti-complement, anti-ulcer, hypoglycemic, hypotens...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00C12N5/0786
CPCC08B37/0003C12N5/0645C12N2501/90
Inventor 曹荣安金成浩罗英花张宇李明博张彤王诗浓李良玉王长远
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com