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Synergistic compound for preventing and treating gray mold of strawberries and application of synergistic compound

A technology of strawberry botrytis cinerea and compound, which is applied in the direction of chemical, application and biocide for biological control, can solve the problems of chemical resistance and other problems, so as to delay the resistance of bacteria and reduce the residue of medicine. , the effect of expanding the antibacterial spectrum

Inactive Publication Date: 2018-11-27
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a synergistic compound for preventing and treating strawberry botrytis and its application in view of the problem that strawberry botrytis is easy to produce resistance to chemical agents, using phenazine-1- The combination of phenazine-1-amide and fluazinam can not only be used for the synergistic control of strawberry botrytis, but also effectively prolong the service life of phenazine-1-amide and fluazinam

Method used

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  • Synergistic compound for preventing and treating gray mold of strawberries and application of synergistic compound
  • Synergistic compound for preventing and treating gray mold of strawberries and application of synergistic compound
  • Synergistic compound for preventing and treating gray mold of strawberries and application of synergistic compound

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Indoor Toxicity Assay

[0016] (1) Drug quality setting

[0017] In an aseptic operating bench, dissolve phenazine-1-carboxamide and fluazinam with a small amount of acetone organic solvent, and prepare a mother solution with a mass concentration of 100 μg / mL with sterile water. Since different pathogenic bacteria are sensitive to different agents, The mycelial growth rate is different, and each agent needs to be pre-tested. Three mass concentrations of 100, 10, and 1 μg / mL are set, and sterile water is used as a control, so that the inhibition rate is in the mass concentration range of 10%-85%. 5 mass concentration gradients were set for the test, and the corresponding mass concentrations of phenazine-1-carboxamide and fluazinam were: 10, 1, 0.1, 0.01, 0.001 μg / mL for Botrytis cinerea.

[0018] (2) The EC of the mixture of phenazine-1-carboxamide and fluazinam against Botrytis cinerea 50 value determination

[0019] The mycelial growth rate method was used. Take...

Embodiment 2

[0028] Example 2 In vitro control effect of phenazine-1-carboxamide and fluazinam on strawberry botrytis

[0029] Weigh phenazine-1-carboxamide and fluazinam original drugs with a balance, dissolve them with an appropriate amount of acetone, dilute with sterile water, and prepare a 500 μg / mL mother solution for future use. In this experiment, there were 4 treatments and 1 water control, namely phenazine-1-carboxamide treatment (250 μg / mL), fluazinam treatment (0.18 μg / mL), phenazine-1-carboxamide and fluazinam Compound treatment (0.23 μg / mL), control drug procymidone treatment (conventional recommended drug concentration) and water control treatment. Apply the prepared medicinal solution evenly on the strawberry fruit, dry it and inoculate it with Botrytis cinerea, repeat 3 times for each treatment, use sterile water as a control, and place it in a dark incubator at 20-23°C for continuous cultivation after drying 72h, adopt cross method to measure bacterium colony diameter, c...

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Abstract

The invention discloses a synergistic compound for preventing and treating gray mold of strawberries and application of the synergistic compound. A mycelial growth rate method is used for determiningphenazine-1-formamide and fluazinam, and the synergistic potential of a mixture of phenazine-1-formamide and fluazinam for preventing and treating the gray mold of the strawberries is evaluated through a Wadley method. Results show that both phenazine-1-formamide and fluazinam have an inhibition function on strawberry gray mold bacteria, wherein the inhibition effect of fluazinam is better than that of phenazine-1-formamide. The two chemicals have a synergistic effect on the strawberry gray mold bacteria in the weight ratio of 1:4 or 3:7 or 2:3 or 1:1 or 7:3 or 4:1 or 9:1, wherein the synergistic effect is optimal when the weight ratio is 9:1. In-vitro prevention and treatment results show that the prevention effect of phenazine-1-formamide and fluazinam on the gray mold of the strawberries reaches 81.63% after the two chemicals are compounded in the weight ratio of 9:1, and the two chemicals can serve as novel chemicals to prevent and treat the gray mold of the strawberries.

Description

technical field [0001] The invention belongs to the field of pesticide compounding, in particular to a synergistic compound compounded with phenazine-1-carboxamide and fluazinam, and at the same time relates to the effect of the compound compound in preventing and treating strawberry gray mold (Botrytis cinerea) application. Background technique [0002] Strawberry gray mold is an important disease that harms strawberry. The disease is mainly caused by the infection of Botrytis cinerea (Botrytis cinerea). In order to effectively control the occurrence of strawberry gray mold, many chemical agents are used in production, but the long-term use of chemical agents in large quantities not only pollutes the environment, but also easily causes the bacteria to develop drug resistance. At present, strawberry botrytis in many areas has developed resistance to procymidone, carbendazim, and diethofencarb, which adds a lot of difficulty to the prevention and control of strawberry botryt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/60A01N43/40A01P3/00
CPCA01N43/60A01N43/40
Inventor 张亚廖晓兰刘双清王翀姚莹
Owner HUNAN AGRICULTURAL UNIV
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