Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

The SNP Molecular Marker Related to Sheep Multiple Lambs and Its Application

A molecular marker and sheep technology, applied in the field of molecular biology, can solve the problems of difficult high-throughput automatic determination, low throughput, and numerous procedures

Active Publication Date: 2021-07-27
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the traditional genotype detection methods use PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) detection methods. These methods have low throughput, numerous procedures, and are difficult to implement High-throughput automated assays

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • The SNP Molecular Marker Related to Sheep Multiple Lambs and Its Application
  • The SNP Molecular Marker Related to Sheep Multiple Lambs and Its Application
  • The SNP Molecular Marker Related to Sheep Multiple Lambs and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Identification of SNP molecular markers associated with sheep lambing

[0050] Through whole-genome resequencing of 99 sheep individuals from 10 sheep breeds, including three breeds of Tibetan sheep (valley-type Tibetan sheep, grassland-type Tibetan sheep, and Euler sheep), and 6 Mongolian sheep (small-tailed Han sheep, Wu Everest, Tan sheep, Hu sheep, Cele black sheep, and Bayinbulak) and 1 European sheep (Australian Merino), obtained the largest number of genetic polymorphic sites in the sheep genome so far, and analyzed these sheep The genetic diversity and population genetic structure of the population were screened for genes related to high altitude adaptability, fat metabolism, multiple lambs and seasonal estrus. A large number of effective SNP sites were obtained through Fst value calculation and some genes were screened, including the SNP sites of the Pappa2 gene related to sheep lambing. It was found that the Pappa2 gene located at the 55865867bp sit...

Embodiment 2

[0051] Example 2 utilizes Sequenom A method for detecting sheep Pappa2 genotype and predicting the average litter size of multiparous ewes by SNP technology

[0052] 1. Experimental materials

[0053] 378 small-tailed Han sheep were selected as the test objects.

[0054] Typing sample selection

[0055] 2. Reagents and instruments

[0056] Reagent: Complete Genotyping Reagent Kit for Compact 384;

[0057] Gene Amplification: ABI 9700 384Dual;

[0058] Mass spectrometry spotting: MassARRAY NanodispenserRS1000;

[0059] Mass spectrometry: MassARRAY Compact System;

[0060] All reagents and instruments were purchased from Beijing Genenode Biotech Co., Ltd.

[0061] 3. Genomic DNA extraction

[0062] 1 mL of blood was collected from the jugular vein of the sheep and treated with EDTA anticoagulant. First, the red blood cell lysate is lysed to remove DNA-free red blood cells. The nucleus lysate lyses the cells to release genomic DNA. Then the protein precipitation sol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a SNP molecular marker related to sheep lambing and an application thereof. The molecular marker is located at position 55865867 (NW_014639021.1) of sheep chromosome 12 and is a Pappa2 gene SNP marker. The invention provides a specific primer combination for detecting the SNP marker, the sequence of the specific primer combination is shown in SEQ ID NO.1-3, based on SequenomSNP technology, the detection of the SNP molecular marker can be realized, and according to the detection The results determined that the sheep Pappa2 gene was homozygous or heterozygous. The invention provides an auxiliary method for sheep breeding. By selecting the Pappa2 genotype of sheep, CG heterozygous individuals and CC homozygous individuals with high lamb size traits can be selected and retained, thereby improving the fecundity of sheep and improving large-scale sheep breeding. Molecular breeding has potential applications.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a SNP molecular marker related to the trait of multiple lambs in sheep and a method for detecting the trait of multiple lambs in sheep. Background technique [0002] The litter size trait is one of the most important economic traits of sheep. Because it is controlled by micro-effect polygenes and has low heritability, it is difficult to improve rapidly by conventional breeding methods. Molecular technology can effectively and rapidly improve its genetic progress, so the identification Major genes or molecular genetic markers related to litter size are the key to modern molecular breeding. [0003] In 2001, Michael T.Overgaard et al. published an article in JBC saying that they expressed the recombinant PAPP-A2 protein in mammalian cells and found that the 1558 amino acid residue polypeptide of the recombinant PAPP-A2 protein was composed of 1791 amino acid residues It ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 储明星胡文萍梁奔梦董新龙刘秋月狄冉王翔宇汤继顺郭晓飞魏彩虹
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com