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A kind of diatom fluorescent staining solution and diatom detection method for detecting visceral tissue

A fluorescent dyeing and inspection method technology, applied in the field of diatom inspection, can solve the problems that diatoms are not easy to be dyed, affect the judgment of corpses, and the diatom morphology is not completely displayed, so as to shorten the detection time, improve work efficiency, and avoid missed detection Effect

Active Publication Date: 2020-09-22
孙鑫 +7
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the cell wall of diatoms is composed of silica (SiO 2 ·nH 2 O) and pectin (pectin), and the silica wraps the colloidal layer on the outer layer. Due to the influence of silica, diatoms are generally not easy to be dyed in this method, resulting in incomplete morphological display of diatoms. , affecting the judgment of the corpse

Method used

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  • A kind of diatom fluorescent staining solution and diatom detection method for detecting visceral tissue
  • A kind of diatom fluorescent staining solution and diatom detection method for detecting visceral tissue
  • A kind of diatom fluorescent staining solution and diatom detection method for detecting visceral tissue

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Embodiment 1

[0032] The diatom fluorescent dyeing solution is composed of the following components in weight percent: 10% fluorescein, 5% mordant, 0.3% hydrofluoric acid, and the rest is deionized water.

[0033] A method for diatom testing using a diatom fluorescent staining solution to detect visceral tissues, comprising the following steps:

[0034] 1) Apply the digestive juice obtained after nitrification of visceral tissues (kidney tissues from drowned corpses) on a glass slide, dry to obtain a smear of the tissue to be tested, and add diatom fluorescence to the smear of the tissue to be tested Put the staining solution in a wet box and place it in a water bath, keep it warm for 20 minutes at 37°C, rinse it with distilled water, put it in a drying machine, and dry it for 4 minutes at 60°C;

[0035] 2) Add decolorization solution to the dried tissue smear in step 1), let it stand for 4 seconds, rinse it with distilled water, put it in a drying machine, dry it at 60°C, and seal the slid...

Embodiment 2

[0041] The diatom fluorescent staining solution is composed of the following components in weight percentage: 10% fluorescein, 5% mordant (aluminum salt), 0.3% hydrofluoric acid, and the rest is deionized water.

[0042] A method for diatom testing using a diatom fluorescent staining solution to detect visceral tissues, comprising the following steps:

[0043] 1) Smear the digestive juice obtained after nitrification of organ tissue (taken from lung tissue) on a glass slide, dry it to obtain a smear of the tissue to be tested, add diatom fluorescent staining solution dropwise to the smear of the tissue to be tested, Put it in a wet box and place it in a water bath, keep it warm for 20 minutes at 37°C, rinse it with distilled water, put it in a drying machine, and dry it for 4 minutes at 60°C;

[0044]2) Add decolorization solution dropwise to the dried tissue smear in step 1), let it stand for 4 seconds, rinse it with distilled water, and dry it at 60°C for 4 minutes. The amo...

Embodiment 3

[0051] A diatom fluorescent staining solution is composed of the following components by weight percentage: 8% fluorescein, 3% mordant (aluminum salt), 0.5% hydrofluoric acid, and the rest is deionized water.

[0052] A method for diatom testing using a diatom fluorescent staining solution to detect visceral tissues, comprising the following steps:

[0053] 1) Apply the digestive juice obtained after nitrification of visceral tissues (kidney tissues from drowned corpses) on a glass slide, dry to obtain a smear of the tissue to be tested, and add diatom fluorescence to the smear of the tissue to be tested Put the staining solution in a wet box and place it in a water bath, keep it warm for 20 minutes at 37°C, rinse it with distilled water, put it in a drying machine, and dry it for 4 minutes at 60°C;

[0054] 2) Add decolorization solution to the dried tissue smear in step 1), let it stand for 4 seconds, rinse it with distilled water, put it in a drying machine, dry it at 60°C,...

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Abstract

The invention discloses a diatom fluorescence staining solution. The diatom fluorescence staining solution is prepared from the following components in percentage by weight: 8 to 12 percent of fluorescein, 3 to 7 percent of mordant (aluminum salt), 0.1 to 0.5 percent of hydrofluoric acid and the balance of deionized water. Meanwhile, the invention provides a diatom test method for detecting visceral tissues. The diatom fluorescence staining solution disclosed by the invention helps to display the diatom morphology; demethicone in the diatom is dissolved by hydrofluoric acid, so that a colloidlayer is exposed; the mordant (aluminum salt) prompts the fluorescein to be marked on the colloid layer; by observing under a fluorescence microscope with the corresponding wavelength, the contour ofthe diatom is clearly manifested under the excitation of ultraviolet light.

Description

technical field [0001] The invention belongs to the technical field of diatom inspection, and in particular relates to a diatom fluorescent staining solution and a diatom inspection method for detecting visceral tissues. Background technique [0002] Drowning is a common case type in forensic practice, and it is also one of the difficult problems in forensic examination. In the practice of prosecuting cases, the laboratory examination of diatoms is particularly important when judging whether the corpse in the water drowned before death or was thrown into the water after death, as well as the place where it fell into the water. [0003] The relevant research on diatom detection of drowned corpses mainly focuses on forensic issues such as whether there are diatoms in the organs and tissues of drowned corpses, and the types of diatoms detected. However, the research progress of diatom detection methods has been slow for a long time. In recent years, with the With the rapid dev...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30G01N21/64
CPCG01N1/30G01N21/643G01N2001/302
Inventor 孙鑫李畅王基锋张广清蒋周莹胡彬彬韩芳芳黄亮
Owner 孙鑫
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