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Saline-alkaline tolerance phosphorus solublizing bacteria strain and application thereof

A technology of phosphorus-dissolving bacteria and salt-alkali tolerance, applied in the field of microorganisms, can solve problems such as difficult colonization, restrictions on the application of phosphorus-dissolving fungal preparations, poor colonization and phosphorus-dissolving effects, etc., to achieve strong adaptability, improve fertility, and grow good effect

Active Publication Date: 2018-11-20
BINZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The living environment of fungi is acidic. Although its phosphorus-dissolving effect is remarkable, it is not easy to colonize after being applied to saline-alkali soil, which restricts the application of phosphorus-dissolving fungal preparations in saline-alkali soil.
There are very few phosphorus-dissolving bacteria with saline-alkali tolerance, and the existing phosphorus-dissolving bacteria have poor colonization and phosphorus-dissolving effects in the saline-alkaline environment

Method used

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  • Saline-alkaline tolerance phosphorus solublizing bacteria strain and application thereof
  • Saline-alkaline tolerance phosphorus solublizing bacteria strain and application thereof
  • Saline-alkaline tolerance phosphorus solublizing bacteria strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0058] Example 1 Acquisition of Phosphorus-Solubilizing Bacteria Strain RPB03

[0059] The acquisition process of phosphate-soluble bacterial strain RPB03 is as follows:

[0060] 1) Enrichment culture: collect the rhizosphere soil in the saline-alkali land of the Yellow River Delta, and carry out enrichment culture in the alkaline PKO medium with a pH value of 8.2 until the Ca in the medium 3 (PO 4 ) 2 all degraded;

[0061] 2) Qualitative screening of phosphate-dissolving strains: Dilute the culture solution obtained in step 1) with 8% normal saline, and dilute it with 10-fold concentration gradient dilution method, and select a dilution concentration of 10 8 -10 12 For coating, draw 100 μl of the above dilution with a 200 μl sterile pipette gun, and spread it on an alkaline PKO agar plate with a pH value of 8.2; Continuous 20 generations on the alkaline PKO medium; 17 primary screened strains that still have phosphorus-dissolving effect were obtained, and the primary st...

Embodiment 2

[0066] The further research of embodiment 2 phosphate-dissolving bacterial strain RPB03

[0067] 1. Effects of pH on phosphorus conversion of phosphate-soluble bacterial strain RPB03

[0068] The preserved strain RPB03 was activated, and cultured in liquid medium for 24 hours as a liquid strain. 1ml (10 8 cfu / ml) seed solution was transferred into PKO medium, and the initial pH values ​​of PKO medium were 6, 7, 8, 9 and 10, respectively, and each treatment was repeated three times. The initial pH was adjusted with hydrochloric acid or NaOH solution. After cultivating on a shaking table for 48 hours, centrifuge at 10,000 rpm / min for 15 minutes, and take the sterile filtrate to measure the available phosphorus content. The results are as follows: figure 2 shown.

[0069] Depend on figure 2 It can be seen that with the increase of the initial pH value of the solution, the phosphorus-dissolving ability of the RPB03 strain continued to decrease, and there was a significant d...

Embodiment 3

[0112] Influence of Indigenous Microorganisms on Phosphorus-Solubilizing Bacteria Strain RPB03

[0113] The RPB03 strain was activated on beef extract-peptone medium (beef extract 3.0g, peptone 10.0g, NaCl 5.0g, agar 15-25g, water 1000ml, pH7.4-8.0), cultured at 28-30°C for 36-48h, Then put it into a 1000mL Erlenmeyer flask, and incubate for 36h at 30°C and 170rpm / min. Inoculate 5% by volume into a 50L seed tank. The culture conditions are 170rpm / min, pH8.0, ventilation 0.6vvm, and cultured for 2 days. After the fermentation is completed, centrifuge, precipitate and dry to obtain bacterial powder. Suspended with tap water to make a concentration of 4.1×10 8 cfu / ml bacterial solution.

[0114] Take two equal amounts of saline-alkali soil in the Yellow River Delta, and set up an experimental group and a control group. The control group was sterilized by dry heat sterilization at a temperature of 170°C for 2 hours, and the soil samples in the experimental group were not treat...

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Abstract

The invention discloses a saline-alkaline tolerance phosphorus solublizing bacteria strain and application thereof, and belongs to the technical field of microorganisms. The saline-alkaline tolerancephosphorus solublizing bacteria strain is named phosphorus solublizing bacteria strain RPB03, which is preserved in the China General Microbiological Culture Collection Center, with a preservation number of CGMCC No:15928, on June 11, 2018. The strain is high in adaptability to a high-salt and high-alkali environment, can increase the effective phosphorus content in calcium phosphate fermented liquid to 430 mg / L and grows well in a saline-alkaline soil environment, and indigenous microorganisms have little impact on the phosphorus solublizing effect of the saline-alkaline tolerance phosphorussolublizing bacteria strain; the effective utilization rate of phosphorus in saline-alkaline soil in a saline-alkaline farmland, a forest and the like can be effectively increased, and the plant biomass is increased. Due to the application of the strain, soil hardening caused by use of chemical fertilizer can be relieved; the utilization rate of soil phosphorus is increased; the environmental pollution is reduced.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a saline-alkali-tolerant phosphorus-dissolving bacterial strain and application thereof. Background technique [0002] China is rich in saline soil resources and widely distributed. According to the second national soil census data, the total area of ​​saline soil in China is about 3600hm 2 , accounting for 4.88% of the country's usable land area. The total area of ​​saline-alkali wasteland and saline-alkali barrier cultivated land in the northwest, northeast and coastal areas of my country exceeds 33.33 million hm 2 (500 million mu). The efficient agricultural utilization of saline-alkali land is of great significance to the improvement of agricultural production capacity in my country, and the shortage and shortage of phosphorus is one of the important factors restricting the low agricultural output value of saline-alkali land. [0003] Phosphorus is a non-renewable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C09K17/14C12R1/01C09K101/00
CPCC09K17/14C09K2101/00C12N1/02C12N1/20C12N1/205C12R2001/01
Inventor 刘萍夏江宝
Owner BINZHOU UNIV
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