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Immunodeficiency nude mouse model constructed by lymphoma cell line

A technology of lymphoma cells and immunodeficiency, which is applied in the field of patent application for immunodeficiency nude mouse models and their construction methods, can solve problems such as the construction of immunodeficiency mouse models that have not been seen, and cannot be stably passed down, and achieve good practical value and the significance of popularization and application, the effect of fast tumor formation and high tumor formation rate

Pending Publication Date: 2018-11-20
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the practical application shows that it is relatively easy to use this construction method to construct animal models of solid xenograft tumors, but when constructing animal models of lymphoma, there are obvious technical defects that cannot be stably passaged
Therefore, there have been no good reports on the construction of immunodeficiency mouse models that can be stably passaged using specific lymphoma cell lines.

Method used

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  • Immunodeficiency nude mouse model constructed by lymphoma cell line
  • Immunodeficiency nude mouse model constructed by lymphoma cell line
  • Immunodeficiency nude mouse model constructed by lymphoma cell line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Take B-cell lymphoma DOHH2, LY8, and Raji cell lines as examples (DOHH2 is follicular cell lymphoma, Ly8 is diffuse large B-cell lymphoma, and Raji is Burkitt cell lymphoma. medium-high B-cell lymphoma type), an immunodeficiency nude mouse model was constructed, and the specific construction process can be found in figure 1 As shown, the details are as follows.

[0053] (1) Pretreat the inoculated subjects and lymphoma cell lines respectively,

[0054] The Balb / c nude mice were pretreated, specifically: before the inoculation of lymphoma cells, the immunosuppressive drug cyclophosphamide was injected; the specific method was that the nude mice were continuously injected intraperitoneally with cyclophosphamide for two days, and the injection volume was calculated as 100 mg / kg ( The injection volume is calculated as 0.2mL), once a day;

[0055] On the day of inoculation, B-cell lymphoma cell lines should be processed as follows:

[0056] Centrifuge the well-growing cel...

Embodiment 2

[0072] Similar to the operation process in Example 1, the inventors also constructed a tumor model that can be stably passaged using NK cell lymphoma cell line (YT cell line). The specific construction process will not be described in detail, but only some results are briefly explained as follows.

[0073] The morphological observation of the formed tumor tissue was carried out, and the results were as follows: Image 6 shown. It can be seen from the figure that diffusely distributed atypical lymphocytes can be seen under the microscope. The cells are large in size, rich in cytoplasm, irregular in karyotype, and thick in chromatin. During the period, there are varying numbers of apoptotic and necrotic cells. Overall, the morphology of the tumor cells was relatively consistent during the passaging process, indicating that the passaging effect was relatively stable.

[0074] Immunohistochemical detection of the expression of some characteristic molecules (CD3ε, CD56, GranzymeB...

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Abstract

The invention belongs to the technical field of animal tumor model construction and specifically relates to an immunodeficiency nude mouse model constructed by a lymphoma cell line and capable of being stably passed and a constructing method thereof. The immunodeficiency nude mouse model constructed by the lymphoma cell line and capable of being stably passed is constructed by using an NK cell lymphoma cell line, a T cell lymphoma cell line or a B cell lymphoma cell line; and the immunodeficiency nude mouse model constructed by the lymphoma cell line and capable of being stably passed comprises processes such as corresponding pre-treatment of an object of inoculation and the lymphoma cell line, preparation of a tumor suspension and injection of a nude mouse and tumor cell passage. The constructing method for constructing a lymphoma animal model by using the NK cell lymphoma cell line, the T cell lymphoma cell line or the B cell lymphoma cell line, provided by the invention, has the advantages that a lymphoma cell can be fixed under the skin by using a fixing effect of matrigel so as to guarantee the tumor formation rate of the nude mouse; technical advantages of fast tumor formation and high tumor formation rate are shown; and on the other hand, in the passage process, the lymphoma animal model for passage can be resuscitated at any time and can be directly constructed under the coordination of freezing operation of relevant tumor tissue culture solution.

Description

technical field [0001] This application belongs to the technical field of animal tumor model construction, and specifically relates to a patent application for an immunodeficiency nude mouse model constructed by using lymphoma cell lines, which can be stably passed down and its construction method. Background technique [0002] The construction of animal tumor models is one of the commonly used research specimen materials in related diseases, especially in tumor research. Among them, the tumor xenograft model has great application value in the screening of new anti-tumor drugs and the evaluation of curative effect, so it is one of the main goals and construction work of animal tumor models. [0003] Immunodeficient animals are implanted with established cell lines or patient tumor tissues to construct tumor xenograft models, which is a commonly used method for constructing tumor models. However, the practical application shows that it is relatively easy to use this construc...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/0271A01K2207/12A01K2217/03A01K2227/105A01K2267/035A01K2267/0393
Inventor 张明智薛伟丽
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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