Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Building method of acute hyperuricemia renal damage mouse model

A technology of hyperuricemia and mouse model, which is applied in the direction of pharmaceutical formulations, organic active ingredients, medical preparations containing active ingredients, etc., and can solve the discomfort of hyperuricemia and kidney damage models Anti-hyperuricemia and kidney damage drugs to reduce uric acid excretion, shorten modeling time, and stabilize serum uric acid levels

Inactive Publication Date: 2018-11-13
WUHAN POLYTECHNIC UNIVERSITY
View PDF1 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to propose a method for constructing a mouse model of acute hyperuricemia renal damage, aiming at solving the problem that the existing hyperuricemia renal damage model is not suitable for use as a model for screening anti-hyperuricemia renal damage drugs The problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Building method of acute hyperuricemia renal damage mouse model
  • Building method of acute hyperuricemia renal damage mouse model
  • Building method of acute hyperuricemia renal damage mouse model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 Construction of acute hyperuricemia renal damage mouse model

[0048] 1. Selection of model animals: 30 male Kunming mice of SPF grade, weighing 25-30 g, provided by the Experimental Animal Center of Huazhong University of Science and Technology, the animal production license number is SCXK (E) 2016-0009, and the breeding environment temperature is 22 ~26°C, humidity 40~60%, mice free access to food and water.

[0049] 2. Drug solution preparation: Hypoxanthine (batch number G1725018, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.), ethambutol hydrochloride (0.25g per capsule, batch number T17A002, Hangzhou Minsheng Pharmaceutical Co., Ltd.), oxazine Potassium acid potassium salt (batch number P137112, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.) and adenine (batch number Y26J8C40561, purchased from Shanghai Yuanye Biotechnology Co., Ltd.), both were prepared into a suspension using 0.5% CMC-Na aqueous solution ,spare...

Embodiment 2

[0064] Example 2 Verification of Allopurinol on Mouse Model of Acute Hyperuricemia Renal Damage

[0065] 1. Selection of model animals: 30 male Kunming mice of SPF grade, weighing 18-22 g, provided by the Experimental Animal Center of Huazhong University of Science and Technology, the animal production license number is SCXK (E) 2016-0009, and the breeding environment temperature is 22 ~26°C, humidity 40~60%, mice free access to food and water.

[0066] 2. Drug solution preparation: Hypoxanthine (batch number G1725018, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.), ethambutol hydrochloride (0.25g per capsule, batch number T17A002, Hangzhou Minsheng Pharmaceutical Co., Ltd.), oxazine Allopurinol (batch number P137112, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.) and allopurinol (batch number Y27O8C46913, purchased from Shanghai Yuanye Biotechnology Co., Ltd.) were both prepared into a suspension with 0.5% CMC-Na aqueous solution. liquid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a building method of an acute hyperuricemia renal damage mouse model. The method comprises the following steps of experiment mice are subjected to molding experiment for many days by using xanthine, ethambutol and oteracil potassium as molding agents; obtaining the acute hyperuricemia renal damage mouse model; observing the body weight, the serum uric acid level and the renal function change of the experiment mice and the kidney pathological change; the renal damage effect of the molding agent on the experiment mice is analyzed. The mice are used as model animals; the serum uric acid level of the mice is equivalent to the uric acid level of a hyperuricemia patient; the serum uric acid level is stable and can easily reappear; meanwhile, the combined effect of uric acid precursors of hypoxanthine is used; the uric acid excreted substances of ethambutol and uricase inhibitors of oteracil potassium are reduced; the acute hyperuricemia renal damage mouse model is built; the molding time is short; the model conforms to the clinical characteristics of human body hyperuricemia renal damage, and is applicable to screening hyperuricemia renal damage resisting medicine.

Description

technical field [0001] The invention relates to the technical field of hyperuricemia animal models, in particular to a method for constructing an acute hyperuricemia renal damage mouse model. Background technique [0002] Hyperuricemia is a type of disease in which the metabolism of purine in the body is abnormal or the function of the kidneys to excrete uric acid is weakened, resulting in excessive production or decreased excretion of uric acid in the body, which in turn leads to a high level of serum uric acid. If the fasting blood uric acid level exceeds 420 μmol / L for men and 360 μmol / L for women on two different days, it is called hyperuricemia, and long-term hyperuricemia often induces kidney damage. The results of investigation and research show that with the improvement of people's living standards, the incidence of hyperuricemia is increasing day by day, and the cases of hyperuricemia renal damage are also increasing. The current drugs for the treatment of hyperuri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/522A61K31/53A61K31/133
CPCA61K31/133A61K31/522A61K31/53
Inventor 徐凌云刘永杰裴忆雪张笛刘德俊
Owner WUHAN POLYTECHNIC UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com