Gingko detoxification tissue culture method

Abstract

The invention discloses a gingko detoxification tissue culture method which comprises the following steps: (1) selecting materials, namely selecting whole strong gingko twigs with full buds of 1-2 years without rotting or disease or insect; (2) carrying out germination acceleration, namely carrying out germination acceleration on the selected gingko twigs; (3) carrying out surface treatment; (4) carrying out sterilization; (5) cutting the twigs; (6) carrying out primary culture; (7) carrying out proliferation subculture, namely inoculating sterile seedlings into a corresponding culture medium,and carrying out proliferation subculture; (8) carrying out rooting culture; (9) carrying out domestication and seedling strengthening. The method has the beneficial effects that through tissue culture of gingko, function of detoxification, rapid propagation, germplasm resource preservation and quality improvement can be achieved, and the quality of gingko can be greatly improved; culture mediumcomponents for controlling differentiation, subculture growth, rooting and the like in tissue culture can be confirmed, types and concentrations of growth regulation substances and optimal optimization selection on culture conditions are studied and confirmed, technical barriers in tissue culture processes of gingko are broken through, and a very good culture effect can be achieved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a ginkgo detoxification tissue culture method. Background technique [0002] Ginkgo biloba, also known as ginkgo, gongsun tree, duck foot and duck palm tree, is a relic plant of the Quaternary glacial period in the Cenozoic Era. It is a rare and precious tree species that only survives in my country. It belongs to one family and is known as the "living fossil" of gymnosperms, and has important scientific value in botany. my country is the hometown of ginkgo, and large-scale planting areas include Shandong, Jiangsu, southern Anhui, Zhejiang, northern Jiangxi, Henan, Hunan, Hubei, Guangxi, and Sichuan. [0003] Ginkgo has the characteristics of material, fruit, medicinal and ornamental. Its material is excellent, not warped or cracked, and suitable for processing. It is a high-grade material for sculpture, construction, furniture, and decoration. The seeds...

AI Technical Summary

Problems solved by technology

Although the technical scheme of the present application directly induces rooting and shortens the seedling cultivation period; it can keep the excellent characters of the parents to the greatest extent. Adding ginkgo branch activated carbon or ginkgo shell activated carbon in the rooting medium can improve the browning phenomenon of tissue culture seedlings, and simultaneously Antibacterial and growth-promoting effects, but only one medium is used in the tissue culture process, which cannot meet the needs of the first generation and proliferation of Ginkgo explants

[0007] There are also some reports on tissue culture research with Ginkgo biloba, but there are problems such as high production cost, high contamination rate of the first generation inoculation, vitrification of test tube seedlings, self-decay of differentiation ability and low survival rate.