A kind of cultivation method of Arabidopsis strong seedling

Abstract

The invention discloses a cultivating method of strong arabidopsis seedlings. The method includes the following steps that filter paper is put in a culture dish, after the filter paper is soaked in clean water, arabidopsis seeds are put on the filter paper, and the filter paper is put in a refrigerator to be vernalized for 2-4 days at the temperature of 3-4 DEG C; the vernalized arabidopsis seedsare sown in nutrition bowls with a seedling culture medium soaked in clean water with a toothpick; 5-6 arabidopsis seeds are sown in each nutrition bowl, the nutrition bowls are covered with preservative films, the nutrition bowls are put in a stainless steel iron pan, then the stainless steel iron pan is put in a culture container to be cultured for 15-20 days, then the preservative films are removed, culture continues for 5-7 days, 4 seedlings with good growth vigor are reserved, and the other seedlings are picked off. After sowing is completed, culture is carried out for 3-4 weeks under condition of the photoperiod of 10-hour illumination and 14-hour darkness, then culture continues under the condition of the photoperiod of 16-hour illumination and 8-hour darkness, and the strong arabidopsis seedlings are obtained. Common materials are adopted, the cultivating steps of arabidopsis are simplified, the cost is reduced, the time is shortened, and the strong seedling rate of the arabidopsis is increased.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for cultivating strong seedlings of Arabidopsis thaliana. Background technique [0002] Arabidopsis belongs to the phylum of angiosperms, dicotyledonous plants, and is a biennial herb. Arabidopsis has the advantages of small plants, short growth cycle, many seeds, small genome, and it is relatively easy to obtain transgenic Arabidopsis plants. Moreover, Arabidopsis is Self-pollination plants are highly homozygous for genes, and the mutation rate is increased by physical and chemical factors, and it is easy to obtain defective types of various metabolic functions. Due to the above advantages, Arabidopsis is a good material for genetics and molecular biology research, and is known as "the fruit fly in plants" by scientists. Arabidopsis thaliana is often used in scientific research. Studies have shown that in the process of transforming Arabidopsis th...

AI Technical Summary

Problems solved by technology

[0003] At present, Arabidopsis thaliana is usually cultivated under sterile conditions and then transplanted into the substrate to continue the cultivation. However, the cultivation under sterile conditions requires the production of plates and seed disinfection, which is cumbersome and requires a lot of time and energy, and there is a risk of contamination. ; and the root system may be damaged during the transplanting process, and there is an obvious delay in the seedling stage, thereby delaying the progress of the test; in addition, the substrates currently used are generally prepared from perlite, vermiculite, and peat, and additional nutrient solution is required. Not only does it increase the cost, but also due to evaporation, the concentration of the nutrient solution in the substrate will gradually increase, causing different degrees of salt stress to Arabidopsis, inhibiting the growth of Arabidopsis, and the proportion of growing robust Arabidopsis is small