A kind of screening method of the SNP marker of Liriodendron tulipifera genome

Abstract

The invention discloses a method for screening the SNP markers of Liriodendron tulipifera genome. The F1 generation individuals obtained by crossing Liriodendron tulipifera 'NK' and Liriodendron tulipifera 'LS' are used as materials, and the reference genome of Liriodendron tulipifera obtained by sequencing is used. Based on this, use Primer5.0 software to design PCR amplification primers and test the amplification efficiency and specificity of the primers, select 27 candidate SNP sites for primer design, and use agarose gel electrophoresis to detect the screening results after PCR amplification , and through the verification of the provenance materials, the SNP markers of the Liriodendron tulipifera genome were finally obtained. The present invention selects 27 SNP sites from the genome database of Liriodendron tulipifera, synthesizes primers, and sends out sequencing after PCR amplification detection. The results show that all sites are real SNP sites, indicating the quality of SNP sites. Reliable, the standard used is good, and the high-quality SNP markers obtained can be used for follow-up work such as genetic linkage maps.

Description

technical field [0001] The invention belongs to the technical field of genomes, and in particular relates to a screening method for SNP markers of Liriodendron tulipifera genome. Background technique [0002] Liriodendron belongs to the genus Liriodendron in the family Magnoliaceae. Today there are only two species, namely Chinese tulipwood (L.chinese Sarg.) (also known as mandarin tree) and North American tulipifera (L.tulipifera L.). Liriodendron has grown in northern Europe, Greenland, and Alaska at higher latitudes in the northern hemisphere in the late Jurassic of the Mesozoic; it was widely distributed in Eurasia and North America in the Tertiary of the Cenozoic; after the glaciers of the Quaternary It is only distributed in the south of my country and the southeast of North America, becoming a typical relict plant. Liriodendron tulipifera and Liriodendron tulipifera have a certain geographical isolation, but their interspecific compatibility is high (Wang Zhangrong, ...

AI Technical Summary

Problems solved by technology

[0003] Since the 1940s, the origin, distribution, phylogenetic status, asexual reproduction, flowering and fruiting habit, and hybrid breeding of Liriodendron have been studied at home and abroad, and molecular markers such as RAPD and SSR have been developed. There are still problems such as the lack of molecular markers suitable for the genetics research of Liriodendron, so the development of SNP markers with high polymorphism will help the investigation and evaluation of genetic resources of Liriodendron plants and the research of hybrid breeding

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