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Application, kit and detection method for novel molecular marker non-coding RNA LINC00958 for lung cancer prognosis

A molecular marker, non-coding technology, applied in the field of tumor molecular biology, to achieve the effect of improving postoperative quality of life, improving survival rate, and profound clinical significance

Inactive Publication Date: 2018-11-06
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether LINC00958 can be used as a new tumor marker to predict the prognosis of lung cancer patients has not been reported

Method used

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  • Application, kit and detection method for novel molecular marker non-coding RNA LINC00958 for lung cancer prognosis
  • Application, kit and detection method for novel molecular marker non-coding RNA LINC00958 for lung cancer prognosis
  • Application, kit and detection method for novel molecular marker non-coding RNA LINC00958 for lung cancer prognosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: The application of non-coding RNA LINC00958, a novel molecular marker for lung cancer prognosis, in the preparation of preparations for predicting the prognosis of lung cancer patients. The marker non-coding RNA LINC00958 is used to prepare preparations for the prognosis of lung cancer patients, and predict the condition of lung cancer patients.

Embodiment 2

[0022] Example 2: Preparation of reagents for detecting the expression of non-coding RNA LINC00958 for the preparation of a kit for the prognosis of lung cancer patients (for 30 reactions) 1. Trizo 120ml

[0023] 2. Inhibit RNA degradation solvent 40ml;

[0024] 3. Chloroform 80ml;

[0025] 4. 80ml of isopropanol;

[0026] 5. DEPC water 10ml;

[0027] 6. 100 μl of a mixture of 10× random primers and Oligo dT primers;

[0028] 7. 150 μl of 5× reverse transcription reaction buffer;

[0029] 8. 100μl of 10mM deoxyribonucleotide triphosphate base dNTP containing Mg2+;

[0030] 9. 200U / μl M-MLV reverse transcriptase 50μl;

[0031] 10. SYBR Green qPCR Mix 500μl;

[0032] 11. 3 μM target gene LINC00958 specific primer (its sequence is shown in SEQ NO:2 and SEQ NO:3) 50 μl;

[0033] 12. 50 μl of 3 μM internal reference gene TUBA1A-specific primer (its sequence is shown in SEQ NO:4 and SEQ NO:5).

Embodiment 3

[0034] Example 3: Detection of non-coding RNA LINC00958 in tissue samples

[0035] 1. Collect the lung cancer or normal control tissues to be tested, wash them with normal saline, put them into a cryopreservation tube filled with a solvent that inhibits RNA degradation, and put them in a -80°C refrigerator for later use.

[0036] 2. Extraction of RNA in tissues:

[0037] (1) Add liquid nitrogen to the mortar first, then cut the tissue into small pieces and grind it into powder in liquid nitrogen, take 100mg of tissue powder with a liquid nitrogen pre-cooled spoon and add it to the EP tube filled with 1ml of Trizol solution middle. The total volume of tissue powder should not exceed 10% of the volume of Trizol used, and it should be mixed thoroughly;

[0038] (2) Leave at room temperature for 5 minutes, then add 200 μl of chloroform, tightly cap the EP tube and shake vigorously for 0.5 minutes. Centrifuge at 12000 rpm for 10 minutes;

[0039] (3) Take the upper aqueous phas...

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Abstract

The invention relates to application, a kit and a detection method for a novel molecular marker non-coding RNA LINC00958 for lung cancer prognosis. Through real-time fluorescent quantitative PCR and survival curve analysis, the non-coding RNA LINC00958 from lung cancer tissues is found to be associated with the survival rate of a patient; the more the content is, the more the survival rate is. Themethod provides a powerful technical support for analyzing prognosis lifetime of cancer patients, favorably improves the postoperative life quality and has profound clinical significance; by means ofthe method, a postoperative treatment scheme is formulated and the survival rate is improved.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and in particular relates to an application, a kit and a detection method of a novel molecular marker non-coding RNA LINC00958 for prognosis of lung cancer. Background technique [0002] Lung cancer, also known as primary bronchial lung cancer, is a malignant tumor originating from the bronchial mucosa and bronchioloalveoli. It is one of the most common malignant tumors in the world today; due to the huge smoking population and the intensification of air pollution such as smog, lung cancer has gradually become the number one cancer in my country. In the past 20 years, the incidence of lung cancer in my country has been increasing at an annual rate of 11%. , Among the first clinically diagnosed cases, 80% are already advanced; the treatment of lung cancer includes surgery, radiotherapy and / or systemic chemotherapy, although a large proportion of patients will still experience recurrence and m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/686C12N15/113
CPCC12Q1/686C12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178C12Q2561/113C12Q2563/107C12Q2545/114
Inventor 田田
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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