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Preparation method of tuna bone collagen peptide chelated calcium

A technology of collagen peptide and tuna, applied in the field of biomedicine, can solve problems such as ineffective application, and achieve the effects of simple operation, strong controllability and high yield

Inactive Publication Date: 2018-11-06
XINGYE ZHOUSHAN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods are all methods of using tuna bone to obtain collagen peptides, and have not been effectively applied to the subsequent chelation of calcium by collagen peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Weigh 40 g of tuna bone powder, the mass ratio of fish bone powder to water is 1:10, cook in distilled water at 100 °C for 5 h under the pressure of 0.10 MPa, and after cooling, add trypsin for enzymatic hydrolysis, the amount of enzyme added is 20000 U / g, the enzymatic hydrolysis temperature is 40 ℃, pH 7.5, and the enzymatic hydrolysis time is 2 h; then pepsin is added for enzymatic hydrolysis, the amount of enzyme added is 15000 U / g substrate, the enzymatic hydrolysis temperature is 35 ℃, pH The enzymatic hydrolysis time was 3.0, and the enzymatic hydrolysis time was 2 h; after the two-step enzymatic hydrolysis was completed, the enzymatic activity was inactivated at 95 °C for 15 min; the enzymatic hydrolysis solution was obtained by suction filtration with three layers of filter paper; the enzymatic hydrolysis solution was dried with a spray dryer to obtain tuna peptide powder;

[0016] (2) The obtained tuna peptide powder was dissolved in distilled water, and ca...

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Abstract

The invention relates to a preparation method of collagen peptide chelated calcium, in particular to a preparation method of tuna bone collagen peptide chelated calcium. According to the method, tunabone collagen peptide and calcium chloride have a chelation reaction to prepare the peptide chelated calcium, the collagen in the tuna bone is effectively utilized, and a product with better calcium supplementation effect is obtained. The method is simple in operation and high in controllability, and the obtained collagen peptide chelated calcium achieves high yield.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a preparation method of collagen peptide chelated calcium, in particular to a preparation method based on tuna bone collagen peptide chelated calcium. Background technique [0002] Tuna is one of the most important pelagic fish species in the world. According to the statistics of the Food and Agriculture Organization of the United Nations, the global annual catch of tuna accounts for more than 70% of the total production of high seas fisheries. With the rise of my country's distant water fishery and the trend of people chasing its deep-sea natural food, the annual catch of tuna in my country has increased year by year, and the consumption of tuna has also increased year by year. In the process of tuna processing, more than 50% of the total weight of scraps are produced, among which tuna fish bones account for a large proportion of these scraps, accounting for more than 20%. These tuna fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/107C12P21/06
CPCC07K14/78C12P21/06
Inventor 劳敏军周小敏李澄胡世伟朱燕芳
Owner XINGYE ZHOUSHAN
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