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Method used for Lonicera fragrantissima in vitro pollen short period storage

A pollen and in vitro technology, applied in the field of plant propagation, can solve the problems of reducing the operability of pollen storage, high requirements for storage conditions, complicated operation process, etc., and achieve the effect of easy to achieve, strong operability and simple conditions

Inactive Publication Date: 2018-11-06
XIAN BOTANICAL GARDEN SHAANXI PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can achieve ultra-low temperature preservation of pollen, it has high requirements for preservation conditions, which are generally difficult to achieve, and the operation process is complicated, which greatly reduces the operability of pollen preservation

Method used

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  • Method used for Lonicera fragrantissima in vitro pollen short period storage
  • Method used for Lonicera fragrantissima in vitro pollen short period storage

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Embodiment 1

[0024] The present invention will be further described in detail by taking the honeysuckle in Xi'an Botanical Garden in Shaanxi Province as an example. The method for short-term storage of isolated pollen of Lonicera japonica described in this embodiment, the isolated pollen used is fresh pollen of Lonicera japonica.

[0025] 1) Harvesting pollen

[0026] Harvesting pollen—on March 10th, select robust and pest-free Eucalyptus japonica plants in the Xi’an Botanical Garden, collect the flowers that loosed the pollen from 9:00 to 10:00 in the morning, put them on sulfuric acid paper, and bring them back quickly In the laboratory, lightly brush the pollen sacs that have loosened powder with a brush to collect pollen.

[0027] 2) Determination of pollen water content

[0028] The water content of the collected fresh pollen is measured by the prior art, and the measurement result shows that the water content of the fresh pollen is 90%.

[0029] 3) Detection of pollen viability

...

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Abstract

The invention belongs to the technical field of plant sexual reproduction, and discloses a method used for Lonicera fragrantissima in vitro pollen short period storage. The method comprises followingsteps: Lonicera fragrantissima fresh pollens released on that very day are collected, pollen germination rate is detected, when it is detected that the pollen germination rate is 80% or higher, the collected fresh pollens are subjected to subpackging with centrifuge tubes, the centrifuge tubes are sealed, and are stored at -20 DEG C, after about 40d of storage, the pollens are taken out from the centrifuge tubes, and the germination rate is detected, wherein the germination rate detected at the second time is usually capable of reaching 40%; at the same time, other parents are right at the full-blossom period, the pollens can be used for artificial supplementary pollination. The method is capable of solving a problem in the prior art that flowering asynchronism is caused in Lonicera fragrantissima cross breeding; operation is simple and convenient; storing cost is low; pollen vitality lasting time is prolonged greatly; it is confirmed by in vitro pollen low temperature storage study that the method possesses important meaning in Lonicera fragrantissima artificial cross breeding.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and relates to a method for storing plant pollen, in particular to a short-term storage method for isolated pollen of Lonicera japonica. Background technique [0002] Tulip Honeysuckle ( Lonicera fragrantissima Lindl. et Paxt.), is a semi-evergreen shrub of Lonicera genus Lonicera, up to 2m high, with a strong fragrance of flowers. It is an important aromatic flower tree in the early spring in the north. In recent years, it is often used in vertical greening, and it is a very good tree species for viewing flowers and fruit gardens. [0003] It has excellent characteristics such as strong floral fragrance and early spring flowering. It can be used as an excellent parent material in the cultivation of new varieties of Lonicera, but usually enters the full bloom stage from mid-February to early March. Other plants of the closely related Lonicera genus (such as honeysuckle ( Lonicera ma...

Claims

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Application Information

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IPC IPC(8): A01N3/00
CPCA01N3/00
Inventor 刘安成尉倩李淑娟王庆柏国清
Owner XIAN BOTANICAL GARDEN SHAANXI PROV
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