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Method for creating ornamental rice germplasm by using two rice zinc finger protein genes

A zinc finger protein and gene technology, applied in the field of plant biology, can solve problems such as non-deterministic causes, and achieve the effect of unique structure, high economic benefit, and simple structure

Inactive Publication Date: 2018-11-06
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although floral organ trait genes play key roles in plant floral organ formation, they are not the only determinants

Method used

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  • Method for creating ornamental rice germplasm by using two rice zinc finger protein genes
  • Method for creating ornamental rice germplasm by using two rice zinc finger protein genes
  • Method for creating ornamental rice germplasm by using two rice zinc finger protein genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Osjag and dl identification and preservation

[0024] Osjag Identification and preservation: such as figure 1 Rice mutants shown in A and 1C Osjag It was obtained by the inventors from the offspring cultured from immature embryos of the indica rice variety Minghui 86 (Duan et al, 2010). like figure 1 Mutants with obvious abnormal development of floral organs shown in A and 1C Osjag , the glumes of the first round of spikelets become narrow, thin, hard and cannot be closed, and the glumes are curved and crescent-shaped; the white, fleshy lodicules of the second round become thinner and larger; the six stamens of the third round are completely Transformation into pistil or fleshy pistil-stamen mosaic; 4th round pistil abnormally developed, ovary with increased stigma and highly sterile. Genetic analysis revealed that the mutant trait is controlled by a recessive single-gene mutation. Sequencing results showed that the mutant OsJAG The gene detect...

Embodiment 2

[0032] Example 2: Double mutants Osjag dl Creation, Rapid Identification and Preservation of

[0033] (1) to carry Osjag The plant of the allele mutant gene is used as the female parent (obtained in Example 1 Osjag mutant heterozygous) with carrying dl Allelic mutant gene and pollen development normal plant (obtained in embodiment 2 dl mutant heterozygote) as the male parent for hybridization to obtain hybrid F1;

[0034] (2) For the F1 obtained in the planting step (1), F2 seeds are harvested from a single plant at the mature stage;

[0035] (3) The F2 obtained in the planting step (2) can be simultaneously isolated from Osjag and dl In the strains of the two mutants, the molecular marker technology was used to quickly identify the normal fruiting and carrying Osjag and dl The heterozygous genotype plants are harvested at the mature stage to obtain the double mutation heterozygous genotype germplasm that can be stably inherited;

[0036] (4) Seeds harvested fr...

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Abstract

The invention relates to the field of biotechnology, and relates to a method for creating an ornamental rice germplasm by using two rice zinc finger protein genes. Mutant alleles of two rice zinc finger-containing domain protein genes OsJAG and DL used as raw materials are introduced into a same indica rice germplasm through hybridization and molecular marker selection to create a rice germplasm that can stably inherit and has high ornamental value. The flower pattern structure of the germplasm is specific, the number of primordia in tetracyclic flower organs is unchanged, but development direction changes obviously, and each spikelet develops into a ''spica-in-flower'' comprising numerous new-abnormally developed spikelets. The outer edges of the spikelets are covered with numerous smallgreen scorpion-like organs, and intermediately filled with pale yellow and fleshy organs. Therefore, the ornamental rice germplasm created by the method has the characteristics such as large and peculiar flower shape, long flowering period, high ornamental value, easy conservation and reproduction, can be used for cultivating ornamental rice, and has good application value and prospects.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for creating ornamental rice germplasm with two zinc finger protein genes of rice. Background technique [0002] Floral organ development in plants is a complex biological process regulated by multiple genes. Over the past 20 years, a series of mutants in Arabidopsis, petunia and snapdragon have been used to carry out research, and the molecular genetic mechanism of flower organ development in dicotyledonous plants has made great progress. , C, D, E" five types of functional genes determine the plant floral organ development model. These five types of genes are dominated by MADS-box, which play a key role in the morphological establishment of flower-planting organs, so they are also called "flower organ characteristics" genes. As the most basic and critical determinants of floral organ formation, most of the characteristic genes of floral organs have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H6/46C12Q1/6895
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 段远霖吴为人
Owner FUJIAN AGRI & FORESTRY UNIV
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