Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Electrochemical biosensor for detecting zeatin and preparation method thereof

A biosensor and electrochemical technology, applied in the field of electrochemical analysis, can solve the problems of high price, long antibody preparation time, and the need for professional operators to improve specificity and specificity, realize instrument miniaturization, and improve detection sensitivity Effect

Inactive Publication Date: 2018-10-30
SHANDONG AGRICULTURAL UNIVERSITY
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bioassays require high sample purity, complex sample pretreatment, and poor specificity and repeatability
Immunoassay methods are prone to interference from cross-reactivity, and antibodies are time-consuming and expensive to prepare
Chromatographic instruments are expensive and complicated to operate, requiring professional operators and other disadvantages

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electrochemical biosensor for detecting zeatin and preparation method thereof
  • Electrochemical biosensor for detecting zeatin and preparation method thereof
  • Electrochemical biosensor for detecting zeatin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1: glassy carbon electrode pretreatment

[0067] The glassy carbon electrode is polished with 30nm aluminum oxide slurry, and then polished with 200-300 mesh aluminum oxide slurry. Then, the glassy carbon electrode was ultrasonically cleaned in deionized water and ethanol for 15 min, respectively. Blow dry with nitrogen.

Embodiment 2

[0068] Embodiment 2: the modification of nano gold

[0069] Immerse the pretreated glassy carbon electrode in 8 mL of 3 mM HAuCl 4 and 0.15M KNO 3 In the mixed solution, use the constant potential deposition technique (potential is -0.3V), and electrodeposit for 250 seconds under magnetic stirring. Then, the electrodes were washed three times with a washing solution. Blow dry with nitrogen. The prepared electrode is labeled as AuNPs / GCE.

Embodiment 3

[0070] Embodiment 3: the modification of probe DNA

[0071] Add 8 μL of probe immobilization buffer (10 mM Tris-HCl, 1.0 mM EDTA, 1.0 M NaCl, 1.0 mM TCEP, pH 7.4) containing 1.0 μM probe DNA to the surface of the AuNPs / GCE electrode, and react at room temperature and under humid conditions After 16 hours, wash 3 times with cleaning solution. The electrodes are labeled: ssDNA / AuNPs / GCE.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses an electrochemical biosensor for detecting zeatin and a preparation method thereof. The electrochemical biosensor comprises an electrode, and nanogold, probe DNA, aptamer DNA,accessory DNA and avidin-modified alkaline phosphatase which are used for modifying on the surface of the electrode in sequence, wherein sequences of the probe DNA, aptamer DNA and accessory DNA are respectively shown as SEQ ID NO. 1-SEQ ID NO. 3. According to the electrochemical biosensor disclosed by the invention, by utilizing excellent biocompatibility and conductivity of the nanogold and catalytic property of specificity of the alkaline phosphatase, the amplification of electrical signals is realized, and the detection sensitivity of the zeatin is improved. By utilizing specific binding and recognizing effects of the aptamer on the zeatin, the zeatin detection specificity is improved. The electrochemical biosensor disclosed by the invention is simple in detection method, capable of realizing instrument miniaturization and easy to operate, and the zeatin can be detected by performing simple treatment on the surface of a glassy carbon electrode only.

Description

technical field [0001] The invention relates to the technical field of electrochemical analysis, in particular to an electrochemical biosensor for detecting zeatin and a preparation method thereof. Background technique [0002] Plant hormones are also known as plant natural hormones or plant endogenous hormones. Refers to some trace organic compounds produced in plants that can regulate (promote, inhibit) their own physiological processes. It is known that there are six types of hormones produced in plants, namely auxin, gibberellin, cytokinin, abscisic acid, ethylene and brassinosterol. They are all simple organic compounds with small molecules, but their physiological effects are very complex and diverse. From affecting cell division, elongation, and differentiation to affecting plant germination, rooting, flowering, fruiting, sex determination, dormancy, and shedding. Therefore, phytohormones play an important role in regulating the growth and development of plants. A...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3278G01N27/48
Inventor 殷焕顺周云雷艾仕云隋程吉王月王明慧
Owner SHANDONG AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com