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Preparation method of platelet lysate rich in CD41<+> and CD81<+> micro-capsules

A technology of platelet lysate and CD81, which is applied to medical preparations containing active ingredients, blood/immune system cells, pharmaceutical formulas, etc., can solve the problems of unrealized platelet regeneration and repair function, low microcapsule content, etc., and achieve regeneration and repair The effect of strong function and simple method

Active Publication Date: 2018-10-30
天晴干细胞股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used clinical method is to use thrombin and calcium ions to activate, and this method releases less than 10 μg / mL of CD41+ and CD81+ microcapsules with regeneration and repair ability in platelet secretions, and microcapsules in platelet lysate The content of cysts is small, so the optimal state of platelet regeneration and repair function has not been realized

Method used

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  • Preparation method of platelet lysate rich in CD41&lt;+&gt; and CD81&lt;+&gt; micro-capsules
  • Preparation method of platelet lysate rich in CD41&lt;+&gt; and CD81&lt;+&gt; micro-capsules
  • Preparation method of platelet lysate rich in CD41&lt;+&gt; and CD81&lt;+&gt; micro-capsules

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specific Embodiment approach 1

[0019] Embodiment 1: In this embodiment, a method for preparing a platelet lysate rich in CD41+ and CD81+ microcapsules is carried out according to the following steps:

[0020] 1. Use a focused extracorporeal shock wave instrument with a frequency of 15Hz and an energy-flux density of 0.15-0.20mJ / mm 2 After shocking platelet-rich plasma or platelet-rich saline 200-500 times at room temperature, incubate at 37 degrees for 10-15 minutes;

[0021] 2. With a frequency of 15Hz and an energy flux density of 0.20-0.25mJ / mm 2 , impact 700-1000 times at room temperature, and incubate at 37 degrees for 20-30 minutes;

[0022] 3. Then, with a frequency of 15Hz and an energy flux density of 0.25-0.30mJ / mm 2 , impact 1500-2000 times at room temperature, and incubate at 37 degrees for 10 minutes to obtain a suspension;

[0023] 4. Put the suspension in step 1 into a cryopreservation tube, freeze it in liquid nitrogen for 20-40 minutes, take it out and shake it in a water bath to melt, r...

specific Embodiment approach 2

[0030] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 1, the platelet concentration in platelet-rich plasma or platelet-rich saline is (6-15)×10 5 individual / μL. Others are the same as those in Embodiment 1 or 2.

specific Embodiment approach 3

[0031] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 or 2 is that the incubation in steps 1, 2 and 3 is at 37° C. 2 performed in the incubator. It is the same as the specific embodiment 1 or 2.

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Abstract

The invention relates to a preparation method of platelet lysate rich in CD41<+> and CD81<+> micro-capsules, and aims to improve the CD41<+> and CD81<+> micro-capsules in the platelet lysate. By meansof impact wave grading impact, the micro-capsules for forming and releasing cell factors and CD41<+> and CD81<+> in platelets are increased. The concentration of the micro-capsules in the platelet lysate is 120 microgram / mL, total protein of the micro-capsules is 7-9 microgram / mL, the particle size of 69.33+ / -2.33% of the micro-capsules is centralized within the range of 30-150nm, the particle size of 15.10+ / -3.20% of the micro-capsules is centralized within the range of 150-300nm, a large number of platelet cell factors can be released, and the preparation method is applied to the technicalfield of biology.

Description

technical field [0001] The invention relates to a preparation method of a platelet lysate rich in CD41+ and CD81+ microcapsules. Background technique [0002] Platelets have been regarded as non-functional cell fragments in the blood for a long time. It was not until 1882 that the Italian physician Bizzozero discovered that they played an important role in the hemostasis process after vascular injury, and it was first proposed that the name of platelets began. Explore the process of platelet coagulation, hemostasis, and maintenance of capillary wall integrity. It is now recognized that platelets have important functions. [0003] Platelets are biologically active small pieces of cytoplasm that are lysed and shed from the cytoplasm of mature megakaryocytes in the bone marrow. They are small in size, 2-3 microns in diameter, and have no nucleus. Platelets have a specific morphological structure and biochemical composition, and have a relatively constant number in normal bloo...

Claims

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Application Information

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IPC IPC(8): C12N5/078A61K38/19A61K35/19A61P17/02
CPCA61K35/19A61K38/19A61P17/02C12N5/0644
Inventor 张怡牛春玮吕秀明刘艳青
Owner 天晴干细胞股份有限公司
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