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Monascus strain capable of high yielding and enriching Monacolin K and application of monascus strain

A technology of Monascus and Monascus, which is applied in the field of microorganisms, can solve the problems of low cleanliness in the production process, different production processes, and different strains, and achieve excellent lipid-lowering effects and good application prospects

Inactive Publication Date: 2018-10-26
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are two main factors that lead to the significant difference in its active ingredients: first, the strains are different. The color koji is mainly produced by the Monascus strain with high pigment production, and the functional red koji is mainly produced by the Monascus strain that can produce lovastatin. Second, the production process is different. Seko is a traditional open-type fermentation. The cleanliness of the production process is not high, and the fermentation product hardly contains lovastatin.

Method used

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  • Monascus strain capable of high yielding and enriching Monacolin K and application of monascus strain
  • Monascus strain capable of high yielding and enriching Monacolin K and application of monascus strain
  • Monascus strain capable of high yielding and enriching Monacolin K and application of monascus strain

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Embodiment 1

[0026] The isolation and identification of embodiment 1 Aspergillus strain of the present invention

[0027] 1. Separation method

[0028] Add 1ml of pollen honey from different sources to 9ml sterile water, and dilute 10ml -1 to 10 -5 , draw different concentrations of dilutions to the pda plate medium, culture at 32°C for 5 days, streak the suspected Monascus colony observed with the naked eye to a new pda plate to form a single colony, and then pick a single colony to the slant medium superior. If the colony is impure, then isolate and purify according to the above method until pure Monascus colonies appear. figure 1 A and B represent the purified ASP-1 and HOP-1 strains, respectively.

[0029] 2. Identification

[0030] 2.1 Identification of colony characteristics

[0031] Configure standard media for Aspergillus identification: Czapek yeast exatractagar (CYA,), Malt extract agar (MEA), 25% glycerol nitrate agar (25% Glycerol nitrate agar , G25N); the culture temper...

Embodiment 2

[0053] Embodiment 2 adopts Monascus of the present invention to prepare red yeast rice

[0054] Using the strains HOP-1 and ASP-1 isolated in Example 1, prepare functional red yeast rice containing monacolin K:

[0055] First prepare the nutrient solution: add 3g of glycerin, 5g of glucose, 1g of yeast, 1g of soybean peptone, MgSO to 100g of rice 4 0.1g, KH 2 PO 4 0.2g, 0.3g of acetic acid, and then add 50-60g of water to adjust the pH of the nutrient solution to 6, put it into a tray, cook at 105°C for 8 minutes at high temperature. After the first cooking, the water content remains at around 35%. After cooking, the grains of rice are crushed and packed in conical flasks so that the grains of rice are 3-5cm thick in the conical flasks. Finally, seal the Erlenmeyer flask with eight layers of gauze, and sterilize at 121° C. for 20 minutes. After the sterilization, take out the Erlenmeyer flask, you can see that the rice grains are scattered among the rice grains, and afte...

Embodiment 3

[0058] Embodiment 3 adopts Monascus of the present invention to prepare red yeast rice

[0059] Prepare nutrient solution: add 10g bran, 5g glucose, 1g yeast, 1g soybean peptone, MgSO to 100g rice 4 0.1g, KH 2 PO 4 0.2 g, 0.3 g of acetic acid, and then add 50-60 g of water to adjust the pH of the nutrient solution to 6, and the rest of the steps are the same as in Example 2.

[0060] The detection is carried out under the condition of high performance liquid phase, and the detected content of monacolin K is stable at 0.8%-1.1%. The light industry standard (QB / T2847-2007) regards lovastatin as the symbolic functional component of functional red yeast rice, and requires that lovastatin must reach more than 0.4%.

[0061] Experimental results prove that the monacolin K content in the red yeast rice prepared by the method of the present invention is high and has strong application value.

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Abstract

The invention discloses a monascus strain capable of high yielding Monacolin K. The strain is a monascus strain, which has a collection number of CGMCC No. 14786 or CGMCC No. 14787 and is collected inGeneral Microorganisms Center of China Committee for Culture Collection of Microorganisms. The invention further discloses application of the strain. The strain disclosed by the invention is high inMonacolin K yield and has a good market application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes; more specifically, it relates to a monascus strain capable of producing high-yield monacolin K and its application. Background technique [0002] Monascus (Monascus) is an important edible and medicinal fungus in my country. It is the production strain of traditional Chinese medicine Monascus, which has a history of thousands of years of application in my country. As a saprophytic fungus, Monascus has an optimum growth temperature of 25°C to 30°C and an optimum pH of 3.5 to 5.0. It can use sugars, organic acids, and alcohols as carbon sources, and nitrate nitrogen, ammonia nitrogen Organic nitrogen is the nitrogen source. Its traditional use is mainly as a food starter, preservative, colorant, flavor enhancer and as a traditional Chinese medicine compatibility. Monascus can secrete a variety of physiologically active substances during the growth and metabolism process, such as: monacolin K (lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/06A23L7/104A23L33/00C12R1/66
CPCA23V2002/00A23L7/104A23L33/00C12P17/06C12N1/145C12R2001/66A23V2200/3262A23V2250/022A23V2250/61A23V2250/76
Inventor 阳泰广兵杨童李晶莹刘进曾艳赵丽许高洁黄胜钟月玲曾婷郭慧杰
Owner CHENGDU MEDICAL COLLEGE
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