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Characteristic markers of different coffee extraction methods and their screening methods and applications

A screening method and marker technology, applied in the field of liquid chromatography-mass spectrometry detection, can solve difficult and unscientific problems, and achieve the effect of accurate identification

Active Publication Date: 2020-09-18
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is very difficult and unscientific to find a reliable marker to identify its brewing method based on a class of compounds originally assumed subjectively.

Method used

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  • Characteristic markers of different coffee extraction methods and their screening methods and applications
  • Characteristic markers of different coffee extraction methods and their screening methods and applications
  • Characteristic markers of different coffee extraction methods and their screening methods and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] A method for discriminating hand-brewed coffee and cold-brewed coffee based on metabolomics, said method comprising the steps of:

[0093] 1. Coffee Sample Preparation

[0094] 1.1 Washed and deep-roasted Arabica coffee bean samples were collected from Ethiopia, Colombia, and Yunnan production areas. After being ground by a grinder, the coffee powder samples were stored in a well-ventilated and light-proof sample storage room at 4°C;

[0095] 1.2 Weigh 2.0±0.001g of each coffee powder sample and prepare coffee samples with different brewing methods according to the solid-liquid ratio of 1:30 by mass. Hand pour coffee: take 2g coffee powder sample in the coffee filter paper and put it on the funnel, take 60ml of boiled pure water and pour it in, take the filtrate within 2min to be the hand brew coffee sample; cold extraction coffee: take 2g coffee powder sample and Mix 60ml of pure water at room temperature, put it in a bottle, and keep it warm at 4°C for 12 hours to ob...

Embodiment 2

[0108] A screening method for identifying characteristic markers of brewed coffee and cold brewed coffee, said method comprising the steps of:

[0109] 1. Coffee Sample Preparation

[0110] 1.1 Washed and deep-roasted Arabica coffee bean samples were collected from Ethiopia, Colombia, and Yunnan production areas. After being ground by a grinder, the coffee powder samples were stored in a well-ventilated and light-proof sample storage room at 4°C;

[0111] 1.2 Weigh 2.0±0.001g of each coffee powder sample and prepare coffee samples with different brewing methods according to the solid-liquid ratio of 1:30 by mass. Brewed coffee: Take 2g of coffee powder sample and 60ml of boiled pure water, mix them in a beaker, put them in boiling water, keep warm for 5min, that is the brewed coffee sample; cold extraction coffee: take 2g of coffee powder sample and 60ml of normal temperature pure water , mixed and placed in a bottle, and kept at 4°C for 12 hours to obtain a cold-brewed coffe...

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Abstract

The invention relates to the field of liquid chromatography / mass spectrometry detection and particularly relates to different coffee extraction method characteristic markers and a screening method andapplication thereof. The method comprises: 1) carrying out grouping according to an extraction method of a coffee sample to obtain standard samples, 2) performing liquid chromatography-mass spectrometry analysis on each group of the standard samples, 3) performing statistical analysis on the obtained data to obtain different substances in the standard samples in each group, and 4) characterizingthe structures of the different substances. The screening method is simple, fast, accurate and stable. The ultra-high performance liquid chromatography tandem quadrupole / time-of-flight high-resolutionmass spectrometry-based non-targeted metabolomics technology is used for identification of coffee. The method has great significance for strengthening the research on the identification of coffee brewing methods, exploring the change of coffee components in the brewing process, improving the quality of coffee and ensuring the health of consumers.

Description

technical field [0001] The invention relates to the field of liquid chromatography-mass spectrometry detection, in particular to characteristic markers of different coffee extraction methods and their screening methods and applications. Background technique [0002] The word "coffee" is derived from the Greek word "Kaweh", which means "strength and enthusiasm". Coffee, tea, and cocoa are known as the three major beverage plants in the world. The global population drinks more than 2 billion cups of coffee every day. It is the second largest commodity in the world, second only to crude oil. The coffee tree is an evergreen small tree belonging to Rubiaceae. Daily coffee is made from coffee beans with various cooking utensils, and coffee beans refer to the nuts in the fruit of the coffee tree that are roasted in an appropriate way. The product. [0003] Global coffee production in 2016 is expected to reach 155,697 thousand packs, with a year-on-year growth rate of 1.5%. In te...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 徐贞贞徐雷王雪陈爱亮杨曙明
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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