Method for producing high maltose syrup by utilizing mpeg-mal5000-β-amylase
A technology of high maltose syrup and amylase, applied in the direction of fermentation, etc., can solve the problems such as β-amylase rarely reported, and achieve the effect of shortening the enzymatic hydrolysis time, lowering the enzymatic hydrolysis temperature, and reducing the production cost
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[0024] 1. Preparation of mPEG-Mal5000-β-amylase
[0025] 1 Materials and methods
[0026] 1.1 Materials and Instruments
[0027] 1.1.1 Reagents
[0028] β-amylase: American Sigma-Aldrich Company, molecular weight 55.7kDa, specific enzyme activity 17,000 U / mg; mPEG-NHS5000, mPEG-NHS20000, mPEG-Ts5000, mPEG-Ts10000, mPEG-Ts20000 and mPEG-Mal5000, Nanocs, USA company, purity ≥95%. All other reagents were of domestic analytical grade.
[0029] 1.1.2 Instruments and equipment
[0030] UV-Vis spectrophotometer: WFJ7200 (Unocal Shanghai Instrument Co., Ltd.);
[0031] Constant temperature water bath: DZKW-4 (Beijing Zhongxing Weiye Instrument Co., Ltd.);
[0032] Oscillating constant temperature metal bath: TUS-200P (Shanghai Yiheng Technology Co., Ltd.).
[0033] 1.2.2 mPEG modified β-amylase reaction
[0034] Add 8.0 mL of disodium hydrogen phosphate-citrate buffer solution of a certain pH to the test tube, then add 1.0 mL of 125 μmol / L β-amylase enzyme solution and 1.0 mL ...
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